Navigation Links
Benchmark Plus Microplate Reader Scan Well Feature

Theresa Redila-Flores and Li Tian, PhD, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA


The Benchmark Plus microplate reader is a new monochromator- based absorbance reader from Bio-Rad that offers superior convenience, accuracy, and flexibility. The Benchmark Plus is controlled by Microplate Manager software. A new feature has been designed in Microplate Manager software (version 5.1 and higher) that utilizes the monochromator design of Benchmark Plus and allows the user to prescan a well in order to determine the optimal wavelength prior to running a full plate of samples. In this note, a prescan procedure is described to show how to implement such a function into real-life applications.

In the following procedure, standard protein (bovine IgG) is added to cell culture medium without serum and the Scan Well function in Benchmark Plus is used to determine the best wavelength for protein assay with cell culture medium. Bio-Rads protein assay kit (catalog # 500-0001) is used to check for measurement inconsistencies in culture medium.


Procedure
1. Serially dilute (1:2) standard bovine IgG protein ranging from 62.5 g/ml to 1 mg/ml in F-12 Hams medium without serum.

2. Aliquot 10 l (in triplicate) of each diluted standard protein into a 96-well clear bottom black microplate; i.e., A1, B1, and C1 for 1 mg/ml protein sample. Aliquot 10 l (in triplicate) of F-12 Hams medium into 3 wells (A7, B7, C7) to be used as blanks.

3. Add 200 l of diluted dye reagent (from Bio-Rad protein assay kit) to each well. Mix thoroughly using a microplate mixer for about 5 sec. Make sure that no bubbles are present. Bubbles may cause incons
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Cell Proliferation Assay Using the Benchmark Plus Microplate Spectrophotometer
2. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
3. Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter
4. Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter
5. Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)
6. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
7. Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)
8. PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)
9. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
10. UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)
11. Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Benchmark Plus Microplate Reader Scan Well Feature

(Date:9/19/2014)... , September 19, 2014 ... latest developments, patents and results of studies and trials. ... Lilly and Company (NYSE: LLY ), Gilead ... (NASDAQ: CELG ) and Arena Pharmaceuticals Inc. ... leader in developing prodrug therapeutics for the treatment of ...
(Date:9/19/2014)... September 19, 2014 , The ... Society for Paediatric Endocrinology (ESPE) Meeting  Investment ... medical research in the field of growth  , ... Germany , today announced the first ... 2014. The awards were announced during a Satellite Symposium ...
(Date:9/18/2014)... -- About POCT POCT, also ... laboratory. It helps in making fast clinical decisions, ... POCT is gaining popularity due to the increasing ... diabetes, heart disease, and obesity. It is generally ... minimize errors during the diagnosis of patients. POCT ...
(Date:9/18/2014)... , September 18, 2014 /PRNewswire/ ... Markets ( http://www.researchandmarkets.com/research/czvg3s/biocompatible ) has announced ... Materials - Global Strategic Business Report" ... (Logo: http://photos.prnewswire.com/prnh/20130307/600769) ,This report analyzes the ... US$ Million by the following Product ...
Breaking Biology Technology:Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 2Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 3Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 4Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 5Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 6Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 7EMD Serono Awards Grant for Growth Innovation (GGI) for the First Time 2EMD Serono Awards Grant for Growth Innovation (GGI) for the First Time 3POCT Market in China 2014-2018 2Biocompatible Materials - Global Strategic Business Report 2014 2Biocompatible Materials - Global Strategic Business Report 2014 3
... 2010 Regenicin , Inc. (OTC Bulletin Board: ... of regenerative cell therapies to restore the health of damaged ... placement. Regencin secured proceeds of $467,550.25. In addition the company ... net of placement agent fees and expenses, will be used ...
... Intarcia Therapeutics. Inc today announced that Kurt Graves, ... at the Lazard Capital Markets 7th Annual Healthcare ... November 16, 2010 at 1:40pm local time at ... (Logo:   http://photos.prnewswire.com/prnh/20050301/SFTU126LOGO ) (Logo: ...
... today announced the receipt of a two-year Small Business ... the National Institute on Aging, National Institutes of Health ... antibodies targeting tau protein oligomers in the development of ... be screened at the Michigan High Throughput Screening Center ...
Cached Biology Technology:Regenicin Announces Closing of Private Placement Transaction 2Intarcia Therapeutics Executive Chairman, Kurt Graves to Present at Lazard Capital Markets 7th Annual Healthcare Conference 2Oligomerix, Inc. Awarded Phase II NIH Grant to Discover Novel Drugs for Alzheimer's Disease 2
(Date:9/18/2014)... have built the first smartphone app that automatically ... trends. In other words, your smartphone knows your ... -- and how that affects you. , The ... and loneliness to their academic performance, also may ... example, to monitor mental health, trigger intervention and ...
(Date:9/18/2014)... Dogs generally seem to be cheerful, happy-go-lucky characters, so ... outlook on life. , In fact some dogs ... University of Sydney shows. , "This research is ... in dogs objectively and non-invasively. It offers researchers and ... and how that changes," said Dr Melissa Starling, from ...
(Date:9/18/2014)... nitrogen comprise all spheres of our planet. Manifold ... imbalances and perturbations that resulted in increases of ... in climate changes which influence the complex biogeochemical ... Understanding these feedback mechanisms has become an important ... the past three decades. , In parallel, a ...
Breaking Biology News(10 mins):New Dartmouth smartphone app reveals users' mental health, performance, behavior 2New Dartmouth smartphone app reveals users' mental health, performance, behavior 3New Dartmouth smartphone app reveals users' mental health, performance, behavior 4Dogs can be pessimists too 2Dogs can be pessimists too 3Greenhouse gas research enters a new era 2
... and muscles around the mouth plays an important role not only ... in the way they are heard according to a study by ... your own face is moving makes a difference in how you ... scientist at Haskins. When, Ito and his colleagues used a ...
... University political science professor is working with Southern ... trade agreements that could threaten the world,s food ... Thompson has been consulting on international agriculture trade ... living in Southern African countries studying agricultural expertise ...
... that face mankind and the environment, there is ... policy, business and education that new, more integrative ... interdisciplinary and multidisciplinary approaches to solving problems at ... at the institutional level. , Integrated Science − ...
Cached Biology News:Feeling your words: Hearing with your face 2New book plants seed for biodiverse food production 2New book plants seed for biodiverse food production 3
The product contains 10 ml Dynabeads Epoxy (4.5 m). Supplied at 4 x 10 8 beads/ml...
Immunogen: Synthetic peptide: M(1) C V P R S G L I L S(11) Storage: -20 C, Avoid Freeze/Thaw Cycles...
...
...
Biology Products: