( Bio-Rad's protein assay kit gave lin...)Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A

Bio-Rad's protein assay kit gave linear results using the Benchmark Plus microplate reader to quantitate protein
concentration. The linear range for Bio-Rads protein assay was between 50 g/ml and 1.0 mg/ml in both 96- and 384-well plates (Figures 1 and 2). Readings were linear within their designated ranges with a correlation coefficient of ≥0.99.

Bio-Rad's detergent compatible (DC) protein assay kit gave linear results using the Benchmark Plus microplate reader to quantitate protein concentration. The linear range for both plates was between 125 g/ml and 2.0 mg/ml in both 96- and 384-well plates (Figures 3 and 4). Readings were linear within their designated ranges with a correlation coefficient of ≥0.99.

Conclusion
The Benchmark Plus microplate reader is an excellent instrument to quantitate concentrations of protein samples
using 2 of Bio-Rads protein assay kits. The correlation coefficients of all the graphs were ≥0.99, within the specified detection ranges of 31.25 g/ml to 1.0 mg/ml. Assays of both
96- and 384-well plate formats gave linear graphs, thus allowing a choice of using these 2 different plate formats with excellent results along with high throughput. The results
showed that the Benchmark Plus microplate reader exhibits excellent linearity, repeatability, and accuracy for use in 96- and 384-well plate formats.

References
Bradford MM, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal Biochem 72, 248254 (1976)

Lowry OH et al., Protein measurement with the Folin phenol reagent, J
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