Navigation Links
Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A

Theresa Redila-Flores, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA


The Bradford and Lowry assays are 2 colorimetric assays that are commonly used to quantitate protein concentration. Bio-Rads protein assay (catalog #500-0001), based on the Bradford dye-binding assay (Bradford 1976), measures total protein concentration. The standard assay is used for protein concentrations ranging from 50 g/ml to 1.0 mg/ml. This assay is based on the change of color of Coomassie Brilliant Blue G-250 dye with different protein concentrations, in which the dye binds to basic and aromatic amino acid residues. This assay is measured between 465 and 595 nm. Bio-Rads detergent compatible (DC) protein assay (catalog #500-0111) is similar to the Lowry assay (Lowry et al. 1951), but only requires a 15 min incubation. The reaction between protein and copper in the alkaline solution and the Folin reagent reduced by the copper-treated protein leads to color development. This protein assay is measured between 650 and 750 nm, with protein concentrations ranging from 200 g/ml to 1.0 mg/ml.


Procedure
Bradford Method (Bio-Rad Protein Assay) Using Clear 96- and 384-Well Plates
1. Dilute dye reagent with 1 part of dye reagent concentrate and 4 parts of distilled water. Filter through a Whatman #1 filter. The diluted solution is good for about 2 weeks when stored at room temperature.

2. Serially dilute standard bovine IgG protein 1:2, ranging from 31 g/ml to 1.0 mg/ml.

3. Aliquot 10 l of each diluted standard in triplicate into a clear 96-well microplate.

4. Add 200 l of diluted dye reagent. Mix thoroughly using a microplate mixer.
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Benchmark Plus Microplate Reader Scan Well Feature
2. Cell Proliferation Assay Using the Benchmark Plus Microplate Spectrophotometer
3. Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter
4. Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter
5. Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)
6. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
7. Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)
8. PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)
9. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
10. UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)
11. Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)
Post Your Comments:
(Date:7/30/2014)... PARK, Md. , July 30, 2014 /PRNewswire-USNewswire/ ... biological components to interrogate biology, but such devices ... biology. As such, researchers have ... for in vitro metabolic engineering to ... on a chip. New devices are envisioned to ...
(Date:7/30/2014)... July 30, 2014  RXi Pharmaceuticals Corporation ... company focused on discovering, developing and commercializing ... using RNA-targeted technologies, today announced that their ... for the reduction of recurrence of hypertrophic ... been initiated. Logo - ...
(Date:7/30/2014)... San Diego, CA (PRWEB) July 30, 2014 ... second contract from the U.S. Environmental Protection Agency ... company’s expertise in developmental toxicity models, including tests ... on neuron formation and the formation of connections ... function. Inhibition of neurodifferentiation and synapse formation by ...
(Date:7/30/2014)... 30, 2014 LayerBio, Inc., an MIT ... its first round of seed financing from friends and ... efforts to develop a novel sustained-release product for ophthalmology. ... investors and their commitment to this important work," said ... is one of many areas in which innovations in ...
Breaking Biology Technology:UMD Researchers Bridge Gap between Microelectronics, Biological Systems 2UMD Researchers Bridge Gap between Microelectronics, Biological Systems 3UMD Researchers Bridge Gap between Microelectronics, Biological Systems 4RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 2RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 3RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 4RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 5U.S. Environmental Protection Agency (EPA) Awards Vala Sciences a Second ToxCast Contract 2U.S. Environmental Protection Agency (EPA) Awards Vala Sciences a Second ToxCast Contract 3
... city of Madison is asking for bids on the construction of ... vendor would be able to charge user fees and would take ... city, county and state for the right to operate. The project ... Twigg said plans and costs are still not finalized. , ,Now, ...
... a success, the Wisconsin Technology Council said ... Wisconsin Governor's Business Plan Contest for state companies ... business proposals within one of four categories: advanced manufacturing, ... will be accepted until January 31st. They will be ...
... to open Thursday , Madison, Wis. The ... on advances in nanotechnology, run by the university and ... on December 16 during a campus open house, with parking ... fiction as the manufacture of tiny robots, has led to ...
Cached Biology Technology:Madison asks for wireless bids; airport, downtown would be first 2Governors business-plan competition starts another round 2Governors business-plan competition starts another round 3Tech Digest: Nanotechnology, GE Healthcare, NovaMed, PhysioGenix 2
(Date:7/30/2014)... effects of climate change on the world,s animal ... factors, according to a new paper by a ... of Queensland, and other organizations. The authors claim ... the point when it comes to climate change. ... scientists focus on the "direct" threats of changing ...
(Date:7/30/2014)... The Governing Board of the Entomological Society of America ... for 2014. The election as a Fellow acknowledges outstanding ... following: research, teaching, extension, or administration. The following Fellows ... Annual Meeting -- which will be held November 16-19, ... BOSQUE-PREZ is a professor with the Department of ...
(Date:7/30/2014)... Sciences Institute (SALSI), a joint venture between The University ... of Texas Health Science Center at San Antonio, has ... The winners, Emily Boice from UTSA and Lei Huang ... their project titled, "Novel engineered ferritins for tracking and ... and Huang,s research is focused on finding a treatment ...
Breaking Biology News(10 mins):Conservation scientists asking wrong questions on climate change impacts on wildlife 2Entomological Society of America recognizes 2014 fellows 2Entomological Society of America recognizes 2014 fellows 3Entomological Society of America recognizes 2014 fellows 4Entomological Society of America recognizes 2014 fellows 5Entomological Society of America recognizes 2014 fellows 6Entomological Society of America recognizes 2014 fellows 7Entomological Society of America recognizes 2014 fellows 8Entomological Society of America recognizes 2014 fellows 9Entomological Society of America recognizes 2014 fellows 10Entomological Society of America recognizes 2014 fellows 11Entomological Society of America recognizes 2014 fellows 12Entomological Society of America recognizes 2014 fellows 13Entomological Society of America recognizes 2014 fellows 14Entomological Society of America recognizes 2014 fellows 15Entomological Society of America recognizes 2014 fellows 16Entomological Society of America recognizes 2014 fellows 17
... CA--Scientists have discovered an unusual symbiosis between tiny single-celled ... important role in marine ecosystems, fertilizing the oceans by ... a form that other organisms can use. ... issue of Science , emerged from the investigation ...
... that traces its origins back to the 1930s, The ... volume of " Intermountain Flora ," which documents in ... Nevada and Rocky Mountains in the American West. Previous ... the preservation of natural resources and wilderness areas in ...
... VIB scientists had serious reservations about the Sralini publication, ... Chemical Toxicology . The conclusions drawn by Sralini could ... have to be subjected to a thorough analysis. ... "Sralini has published similar accounts before, but not one ...
Cached Biology News:Unusual symbiosis discovered in marine microorganisms 2Unusual symbiosis discovered in marine microorganisms 3Unusual symbiosis discovered in marine microorganisms 4NYBG press publishes final volume of landmark 'Intermountain Flora' series 2NYBG press publishes final volume of landmark 'Intermountain Flora' series 3NYBG press publishes final volume of landmark 'Intermountain Flora' series 4NYBG press publishes final volume of landmark 'Intermountain Flora' series 5
PFK-2 car (P-17)...
... product contains 5 ml Dynabeads Epoxy (4.5 ... ether (epoxy) reactive groups. Ideal for coupling ... absorbed hydrophobically on initial coupling with covalent ... and sulphydryll groups (SH)) occurring within ...
LTBP-2 (7B10)...
... monoclonal [198C679.2.1] to Presenilin 2 Alzheimer's ... of the disease carry mutations in the ... precursor protein (APP). These disease-linked mutations result ... of amyloid-beta (main component of amyloid deposits ...
Biology Products: