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Bacterial mRNA Isolation, Fast and Easy

ock MICROBExpress procedure without the Capture Oligo Mix (total RNA). The electropherograms above were generated by running each RNA sample on an Agilent 2100 bioanalyzer using the RNA LabChip. Aligning the traces reveals >95% removal of the 16S and 23S rRNA peaks in the mRNA purified with the MICROBExpress Kit. (Note that the peak corresponding to 5S rRNA and tRNAs is not affected by the MICROBExpress procedure.)


Using total RNA that lacks small RNA molecules (tRNA and 5S rRNA) as the starting material, the MICROBExpress procedure produces the highest possible level of mRNA enrichment. Small RNAs can be removed during total RNA isolation by using our RiboPure Bacteria Kit. Alternatively, small RNAs can be removed from existing total RNA samples using Ambion's MEGAclear Kit.

You can also view a list of organisms that are compatible with the MICROBExpress Kit.

To extend the kit's compatibility with other microbes, Ambion now offers a MICROBExpress Module for Cyanobacteria mRNA isolation as well as MICROBExpress Modules for Pseudomonas, Campylobacter, Helicobacter, and Rhodobacter mRNA isolation.


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