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Bacterial mRNA Isolation, Fast and Easy

NEW Cyanobacterial Module


MICROBExpress (patent pending) is currently the only commercially available bacterial mRNA purification kit. It uses a novel strategy that rapidly removes >95% of the 16S and 23S rRNA from total RNA of E. coli and other bacterial species (Figure 1). MICROBExpress can be used with total RNA obtained by a variety of methods, including glass fiber filter-based methods (such as Ambion's RNAqueous Kits), and phenol-based methods (such as Ambion's RiboPure-Bacteria Kit). The mRNA isolated with MICROBExpress is a superior template for synthesizing labeled cDNA for array analysis and is ideal for quantitative RT-PCR, Northern applications, and cDNA library construction.


Quick and Specific Removal of rRNA
In the first step of the MICROBExpress procedure, total RNA is mixed with an optimized set of capture oligonucleotides that bind to bacterial 16S and 23S rRNAs. Next, the rRNA hybrids are removed from the solution using derivatized magnetic microbeads. The mRNA remains in the supernatant and is recovered by ethanol precipitation. In addition to purifying bacterial mRNA that is virtually free of 16S and 23S rRNA, this method also delivers the benefits of quick processing (less than 2 hours), ease of handling, and scalability.

Figure 1. Depletion of Bacterial 16S and 23S rRNA with MICROBExpress. 16S and 23S rRNAs were selectively removed from a 10 g E. coli total RNA sample using the MICROBExpress Kit. A second 10 g sample of the same total RNA prep was subjected to a m
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