Navigation Links
Bacterial mRNA Isolation, Fast and Easy

NEW Cyanobacterial Module


MICROBExpress (patent pending) is currently the only commercially available bacterial mRNA purification kit. It uses a novel strategy that rapidly removes >95% of the 16S and 23S rRNA from total RNA of E. coli and other bacterial species (Figure 1). MICROBExpress can be used with total RNA obtained by a variety of methods, including glass fiber filter-based methods (such as Ambion's RNAqueous Kits), and phenol-based methods (such as Ambion's RiboPure-Bacteria Kit). The mRNA isolated with MICROBExpress is a superior template for synthesizing labeled cDNA for array analysis and is ideal for quantitative RT-PCR, Northern applications, and cDNA library construction.


Quick and Specific Removal of rRNA
In the first step of the MICROBExpress procedure, total RNA is mixed with an optimized set of capture oligonucleotides that bind to bacterial 16S and 23S rRNAs. Next, the rRNA hybrids are removed from the solution using derivatized magnetic microbeads. The mRNA remains in the supernatant and is recovered by ethanol precipitation. In addition to purifying bacterial mRNA that is virtually free of 16S and 23S rRNA, this method also delivers the benefits of quick processing (less than 2 hours), ease of handling, and scalability.

Figure 1. Depletion of Bacterial 16S and 23S rRNA with MICROBExpress. 16S and 23S rRNAs were selectively removed from a 10 g E. coli total RNA sample using the MICROBExpress Kit. A second 10 g sample of the same total RNA prep was subjected to a m ock MICROBExpress procedure without the Capture Oligo Mix (total RNA). The electropherograms above were generated by running each RNA sample on an Agilent 2100 bioanalyzer using the RNA LabChip. Aligning the traces reveals >95% removal of the 16S and 23S rRNA peaks in the mRNA purified with the MICROBExpress Kit. (Note that the peak corresponding to 5S rRNA and tRNAs is not affected by the MICROBExpress procedure.)


Using total RNA that lacks small RNA molecules (tRNA and 5S rRNA) as the starting material, the MICROBExpress procedure produces the highest possible level of mRNA enrichment. Small RNAs can be removed during total RNA isolation by using our RiboPure Bacteria Kit. Alternatively, small RNAs can be removed from existing total RNA samples using Ambion's MEGAclear Kit.

You can also view a list of organisms that are compatible with the MICROBExpress Kit.

To extend the kit's compatibility with other microbes, Ambion now offers a MICROBExpress Module for Cyanobacteria mRNA isolation as well as MICROBExpress Modules for Pseudomonas, Campylobacter, Helicobacter, and Rhodobacter mRNA isolation.


back to top


Ordering Information
Cat# Product Name Size 1905 MICROBExpress Bacterial mRNA Enrichment Kit 20 rxns 1925 RiboPure-Bacteria Kit 50 purifications 5750 MICROBExpress Module: Pseudomonas 20 purifications 5751 MICROBExpress Module: Campylobacter/Helicobacter 20 purifications 5752 MICROBExpress Module: Rhodobacter 20 purifications 5753 MICROBExpress Module: Cyanobacteria 20 purifications
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. High-Throughput Cultivation of Bacterial Clones
2. Mammalian and Bacterial Expression in One Vector
3. Bacterial RNA Isolation From Infected Eukaryotic Hosts
4. Bacterial RNA Isolation From Infected Eukaryotic Hosts
5. Bacterial Whole Genome Array Analysis
6. Perform Microarray Analysis with Limited Bacterial RNA
7. RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis
8. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
9. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
10. Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes
11. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/8/2016)... , February 8, 2016 ... Private Limited, an innovation-driven oncology company developing next ... less toxic, today announced that chairman emeritus of ... invested in the company as part of the ... joining existing investors Navam Capital and Aarin Capital. ...
(Date:2/8/2016)... , ... February 08, 2016 , ... Information Management Services ... product. The upgrade represents a completely new technical foundation and is so significant ... a responsive design interface, significantly increased speed for search results, a streamlined layout and ...
(Date:2/8/2016)... has an active R&D program for the development of future ... has a unique research and development center in ... Control products. Stockton has a variety ... guidelines, and is active in more than 35 counties worldwide. ... Stockton,s flagship product Timorex Gold ® is ...
(Date:2/6/2016)... ... February 06, 2016 , ... Contact:, Abby Mitchell, Communications ... in Education Sponsors Teacher Training Program , Bite of Science Dinner Event to ... for Excellence in Education (CEE) will sponsor a Bite of Science professional enrichment ...
Breaking Biology Technology:
(Date:2/2/2016)... Feb. 2, 2016  Based on its recent ... Sullivan recognizes US-based Intelligent Retinal Imaging Systems (IRIS) ... Award for New Product Innovation. IRIS, a prominent ... North America , is poised to set ... diabetic retinopathy market. The IRIS technology presents superior ...
(Date:2/1/2016)... -- Wocket® smart wallet ( www.wocketwallet.com ) announces the launch of a new ... Las Vegas , where Joey appeared at the ... Las Vegas , where Joey appeared at the Wocket booth to ... was filmed at the Consumer Electronics Show (CES2016) in Las ... and greet fans. --> --> ...
(Date:1/25/2016)... BLUE BELL, Pa. , Jan. 25, 2016   Unisys ... facial recognition system at John F. Kennedy (JFK) International Airport, ... and Border Protection (CBP) identify imposters attempting to enter ... or do not belong to them. pilot testing ... rolled out initially at three terminals at JFK during January 2016. ...
Breaking Biology News(10 mins):