Navigation Links
Bacterial mRNA Isolation, Fast and Easy

NEW Cyanobacterial Module


MICROBExpress (patent pending) is currently the only commercially available bacterial mRNA purification kit. It uses a novel strategy that rapidly removes >95% of the 16S and 23S rRNA from total RNA of E. coli and other bacterial species (Figure 1). MICROBExpress can be used with total RNA obtained by a variety of methods, including glass fiber filter-based methods (such as Ambion's RNAqueous Kits), and phenol-based methods (such as Ambion's RiboPure-Bacteria Kit). The mRNA isolated with MICROBExpress is a superior template for synthesizing labeled cDNA for array analysis and is ideal for quantitative RT-PCR, Northern applications, and cDNA library construction.


Quick and Specific Removal of rRNA
In the first step of the MICROBExpress procedure, total RNA is mixed with an optimized set of capture oligonucleotides that bind to bacterial 16S and 23S rRNAs. Next, the rRNA hybrids are removed from the solution using derivatized magnetic microbeads. The mRNA remains in the supernatant and is recovered by ethanol precipitation. In addition to purifying bacterial mRNA that is virtually free of 16S and 23S rRNA, this method also delivers the benefits of quick processing (less than 2 hours), ease of handling, and scalability.

Figure 1. Depletion of Bacterial 16S and 23S rRNA with MICROBExpress. 16S and 23S rRNAs were selectively removed from a 10 g E. coli total RNA sample using the MICROBExpress Kit. A second 10 g sample of the same total RNA prep was subjected to a mock MICROBExpress procedure without the Capture Oligo Mix (total RNA). The electropherograms above were generated by running each RNA sample on an Agilent 2100 bioanalyzer using the RNA LabChip. Aligning the traces reveals >95% removal of the 16S and 23S rRNA peaks in the mRNA purified with the MICROBExpress Kit. (Note that the peak corresponding to 5S rRNA and tRNAs is not affected by the MICROBExpress procedure.)


Using total RNA that lacks small RNA molecules (tRNA and 5S rRNA) as the starting material, the MICROBExpress procedure produces the highest possible level of mRNA enrichment. Small RNAs can be removed during total RNA isolation by using our RiboPure Bacteria Kit. Alternatively, small RNAs can be removed from existing total RNA samples using Ambion's MEGAclear Kit.

You can also view a list of organisms that are compatible with the MICROBExpress Kit.

To extend the kit's compatibility with other microbes, Ambion now offers a MICROBExpress Module for Cyanobacteria mRNA isolation as well as MICROBExpress Modules for Pseudomonas, Campylobacter, Helicobacter, and Rhodobacter mRNA isolation.


back to top


Ordering Information
Cat# Product Name Size 1905 MICROBExpress Bacterial mRNA Enrichment Kit 20 rxns 1925 RiboPure-Bacteria Kit 50 purifications 5750 MICROBExpress Module: Pseudomonas 20 purifications 5751 MICROBExpress Module: Campylobacter/Helicobacter 20 purifications 5752 MICROBExpress Module: Rhodobacter 20 purifications 5753 MICROBExpress Module: Cyanobacteria 20 purifications
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. High-Throughput Cultivation of Bacterial Clones
2. Mammalian and Bacterial Expression in One Vector
3. Bacterial RNA Isolation From Infected Eukaryotic Hosts
4. Bacterial RNA Isolation From Infected Eukaryotic Hosts
5. Bacterial Whole Genome Array Analysis
6. Perform Microarray Analysis with Limited Bacterial RNA
7. RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis
8. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
9. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
10. Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes
11. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Bacterial mRNA Isolation Fast and Easy

(Date:9/30/2014)... , Sept. 30, 2014 Report Details ... with highest potential revenues Do you want to ... gives you revenue predictions for those biological drugs ... therapies you find financial data, R&D trends, opportunities ... get sales forecasts to 2024 at overall world ...
(Date:9/30/2014)... (PRWEB) September 30, 2014 Over the ... primarily due to rising R&D budgets and increased acceptance ... fast growing pharmaceutical and biotechnology industries are also catalyzing ... laboratory filtration market is expected to grow at a ... is estimated to be worth $2,532.6 million by 2019. ...
(Date:9/30/2014)... Sept. 30, 2014  RegeneRx Biopharmaceuticals, Inc. (OTC: ... it has received a Canadian Patent for Inhibition ... This patent includes claims using thymosin beta 4, ... peptides for these purposes.  The patent will expire ... Inc. ( www.regenerx.com ) RegeneRx ...
(Date:9/30/2014)... September 30, 2014 Genedata, ... for drug discovery and related life science ... has chosen Genedata Selector as its host-pathogen ... data. FUNGITECT is a European consortium dedicated ... diseases via individualized anti-fungal drug therapies. Genedata ...
Breaking Biology Technology:Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 4Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 5Global Laboratory Filtration Market To See 7.0% CAGR to 2019 Says a Research Report Available at ReportsnReports.com 2Global Laboratory Filtration Market To See 7.0% CAGR to 2019 Says a Research Report Available at ReportsnReports.com 3Global Laboratory Filtration Market To See 7.0% CAGR to 2019 Says a Research Report Available at ReportsnReports.com 4Global Laboratory Filtration Market To See 7.0% CAGR to 2019 Says a Research Report Available at ReportsnReports.com 5RegeneRx Receives Canadian Patent for Inhibition or Reversal of Skin Aging 2FUNGITECT Consortium Adopts Genedata Selector as Data Analysis Platform for Anti-fungal R 2FUNGITECT Consortium Adopts Genedata Selector as Data Analysis Platform for Anti-fungal R 3
... AAFS Convention -- With,tomorrow,s release of the Forensics ... Forensic Science Organizations (CFSO) has announced that: , ... be available to the media for comment,on the Forensics ... of,Sciences on Wednesday, February 18th at 1:00 pm EST. ...
... Idenix Pharmaceuticals, Inc. (Nasdaq: IDIX ), a biopharmaceutical ... the treatment of human viral and other infectious diseases, today ... ended December 31, 2008. At December 31, 2008, Idenix,s cash, ... , , ...
... Even Among Companies Doing Well, Relationships Weaken in Many CountriesNEW ... physicians in four out of the five major European countries ... top marks for effectively delivering key sales and service activities. ... ties with Novartis in the UK and Germany. In ...
Cached Biology Technology:CFSO Calls for Roundtable to Develop National Strategy for Advancing Forensic Sciences in the United States 2Idenix Pharmaceuticals Reports Fourth Quarter and Year End Financial Results 2Idenix Pharmaceuticals Reports Fourth Quarter and Year End Financial Results 3Idenix Pharmaceuticals Reports Fourth Quarter and Year End Financial Results 4Idenix Pharmaceuticals Reports Fourth Quarter and Year End Financial Results 5Idenix Pharmaceuticals Reports Fourth Quarter and Year End Financial Results 6Idenix Pharmaceuticals Reports Fourth Quarter and Year End Financial Results 7Physicians in Europe Rate Novartis #1 on Service Delivery, While Merck Earns Top Honors in the US 2Physicians in Europe Rate Novartis #1 on Service Delivery, While Merck Earns Top Honors in the US 3Physicians in Europe Rate Novartis #1 on Service Delivery, While Merck Earns Top Honors in the US 4Physicians in Europe Rate Novartis #1 on Service Delivery, While Merck Earns Top Honors in the US 5Physicians in Europe Rate Novartis #1 on Service Delivery, While Merck Earns Top Honors in the US 6Physicians in Europe Rate Novartis #1 on Service Delivery, While Merck Earns Top Honors in the US 7
(Date:9/30/2014)... HEIGHTS, Ill. (September 30, 2014) People who suffer ... can do to control their asthma besides properly taking ... a new article in the Annals of Allergy, ... American College of Allergy, Asthma and Immunology (ACAAI), asthma ... examining their lifestyle. The woman described in the Annals ...
(Date:9/29/2014)... , coupled with a high-fat diet, may cause animals to ... mBio , the online open-access journal of the American Society ... Institute of Human Nutrition Potsdam-Rehbruecke in Nuthetal observed that mice ... weight when fed a high-fat diet. Mice that did not ... consuming a high-fat diet, and mice that had C. ...
(Date:9/29/2014)... -- Plants have a symbiotic relationship with certain ... nutrients and defend against invaders an important ... vegetables. Now, scientist have discovered that plants may ... ensuring that sprouting plants are colonized from the ... Dame, presented their findings today at the 5th ...
Breaking Biology News(10 mins):Asthma symptoms kicking up? Check your exposure to air pollution 2Gut bacteria promote obesity in mice 2Plants prepackage beneficial microbes in their seeds 2
... common knowledge that smoking is a health risk but why ... In an evaluation for Faculty of 1000 Biology, Neil Grunberg ... Grunberg describes the study, published by Natividad et al. ... "may have implications to help understand why adolescents are particularly ...
... of hake, lumpsucker and salmon is the best dietary source of ... the University of Almera (UAL). The scientists analysed the eggs, or ... high levels of these fatty acids, which are essential to the ... of the nutritional potential of the roe of marine animals, but ...
... nations are gathered at the two-week UN Climate ... distinguished guests attended an ESA-hosted side event entitled ... Variables,. During the event, speakers from various ... play in providing systematic global climate observations. These ...
Cached Biology News:Roe of marine animals is best natural source of omega-3 2Space agencies join forces to systematically observe climate variables 2Space agencies join forces to systematically observe climate variables 3
MOUSE ANTI SIMIAN VIRUS 5 Immunogen: Simian virus 5...
... Dog Serum-Mixed Breed • Dog serum is collected ... one year of age Available Anticoagulants: ... (Na) N-06: K2EDTA N-08: ... N-03: Alsevers N-05: ACD ...
Ready-to-use; 40mg/ml X-Gal and 32mg/ml IPTG...
Whole blood is collected from canines (any breed, either sex), refrigerated within 6 hours, processed within 24 hours and frozen at -20C....
Biology Products: