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Bacterial Whole Genome Array Analysis

isolated total RNA; this sample was labeled and hybridized using the same protocol as in Panel A. The total RNA (average between duplicate GeneChips) gave 73% Present Calls (genes above background) versus MICROBExpress isolated mRNA which gave 80% Present Calls, thus demonstrating an increase in sensitivity. In addition, the number of Concordant Calls for duplicate GeneChips increased with MICROBExpress mRNA (94%) compared with total RNA (92%). Concordant Calls are those genes called identically on both arrays (Present/Present, Marginal/Marginal or Absent/Absent), and is a direct measure of reproducibility.


*Correlation coefficients were >0.99 for both replicate comparisons.

GeneChip is a registered trademark of Affymetrix, Inc.


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Ordering Information Cat# Product Name Size 1905 MICROBExpress Bacterial mRNA Enrichment Kit 20 rxns 1907 TURBO DNA-free 50 rxns 1925 RiboPure-Bacteria Kit 50 purifications 2238 TUR
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