Navigation Links
Bacterial Whole Genome Array Analysis

Figure 1. Agilent Bioanalyzer Image of E. coli mRNA Purified Using MICROBExpress. 16S and 23S rRNAs were selectively removed from five different 10 g samples of E. coli total RNA and the remaining enriched mRNA fraction (~70 ng each) were run in Lanes 2-6. A sixth 10 g sample of the same total RNA prep was subjected to a mock MICROBExpress procedure without using the kit's rRNA capture oligo mix. Approximately 0.7 g micrograms of the mock reaction was loaded in Lane 7. As seen in the gel image the rRNA bands are virtually undetectable in the mRNA obtained with the MICROBExpress procedure versus the control lane.

Figure 2. Agilent Bioanalyzer Electropherograms of mRNA from Figure 1. As seen in the (top) electropherogram, greater than 95% of the bacterial 16S and 23S rRNA bands have been removed, leading to a selective enrichment of mRNA species as compared to the (bottom) mock MICROBExpress procedure. (Relative fluorescence units have been rescaled between the two electropherograms to allow a closer examination of the MICROBExpress enriched RNA).

Figure 3. Affymetrix GeneChip E. coli Antisense Genome Array Analysis. Illustrated above are Log Plots (Signal vs. Signal) for technical replicate GeneChips (i.e. same RNA, different probe labeling used for replicates). Panel A compares replicate arrays using 10 g RiboPure-Bacteria isolated E. coli total RNA with the standard Affymetrix labeling and hybridization protocol. Panel B compares replicate arrays utilizing the mRNA (0.69 g) obtained with the MICROBExpress Kit from 10 g of RiboPure-Bacteria
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. High-Throughput Cultivation of Bacterial Clones
2. Mammalian and Bacterial Expression in One Vector
3. Bacterial mRNA Isolation, Fast and Easy
4. Bacterial RNA Isolation From Infected Eukaryotic Hosts
5. Bacterial RNA Isolation From Infected Eukaryotic Hosts
6. Perform Microarray Analysis with Limited Bacterial RNA
7. RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis
8. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
9. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
10. Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes
11. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Bacterial Whole Genome Array Analysis

(Date:5/6/2015)... OR (PRWEB) May 06, 2015 ... search and growth strategy for the specialty pharmacy industry, ... of the 2015 Armada Specialty Pharmacy Summit. The Summit ... Hotel in Las Vegas and is the largest annual ... will be in attendance, from pharmacy providers, pharma/biotech manufacturers, ...
(Date:5/5/2015)... May 5, 2015 Roka Bioscience, Inc. (NASDAQ: ... testing solutions for the detection of foodborne pathogens,  today announced ... Bank of America Merrill Lynch 2015 Health Care Conference on ... at Encore at the Wynn, Las Vegas NV. ... presentation will be available through the investor relations section of ...
(Date:5/5/2015)... , May 05, 2015 Research and ... announced the addition of the "Separation ... their offering. Macromolecules which include ... separation or are broken into smaller molecules, ... This separation requires various advanced systems like ...
(Date:5/5/2015)... SAN DIEGO , May 5, 2015 /PRNewswire-USNewswire/ ... treatments for congestive heart failure and other chronic ... create an independent analysis designed to reflect the ... over time. This model will be used to ... for CHF, could have on those groups of ...
Breaking Biology Technology:The Remedy Group presenting at the 2015 Armada Specialty Pharmacy Summit: The Pitfalls to Growth and Maintaining the Patient Experience 2Global Separation Systems Market in Biotechnology 2015-2019 - Trends and Forecasts for the $28 Billion Market 2RAND Health Advisory Services modeling heart failure patient outcomes to inform potential impact of Renova Therapeutics lead product candidate 2RAND Health Advisory Services modeling heart failure patient outcomes to inform potential impact of Renova Therapeutics lead product candidate 3
... exclusive, distribution agreement with AZPA International Inc. for Australia and New ... ... LAVAL, QC, Dec. 19 /PRNewswire-FirstCall/ - Neptune Technologies &,Bioressources Inc. ("Neptune") ... an exclusive distribution agreement with,AZPA International Inc. ("AZPA International") for Australia ...
... collaborators, LineaGen is leveraging new funding round, Utah Population ... platform for commercializing first-in-class molecular diagnostic ... ... Inc.,( http://www.LineaGen.com ), a biomarker discovery company created to,discover and commercialize ...
... the Announcement of a Tracking Program for,Cloned Livestock, ... a,statement by International Dairy Foods Association President and ... a new national registry to track cloned,livestock as ... over food,products from cloned animals. However, we strongly ...
Cached Biology Technology:Nationwide product launch of NKO(R) planned for February 2008 in Australia and New Zealand 2Nationwide product launch of NKO(R) planned for February 2008 in Australia and New Zealand 3LineaGen Inc. Launches With Close of $5.8 Million Series A Funding Round 2LineaGen Inc. Launches With Close of $5.8 Million Series A Funding Round 3LineaGen Inc. Launches With Close of $5.8 Million Series A Funding Round 4Statement of International Dairy Foods Association President and CEO Connie Tipton 2
(Date:4/20/2015)... The announcement comes as demand for fire ... Ireland,s foremost records management company, has ... an impressive track record of clients within the first 12 ... management sector in Dubai . As a ... employ a further eight staff members at its Irish headquarters. ...
(Date:4/14/2015)... YORK , April 14, 2015  HYPR ... IDentity Online (FIDO ® ) Alliance tm , ... specifications. FIDO members commit to share technology and ... methods that are interoperable, more secure and private, ... enable biometric identity verification that protects sensitive user ...
(Date:4/13/2015)... , April 13, 2015 ... TechSci Research, "Global Biometrics Market Forecast & Opportunities, 2020", ... a CAGR of around 14% till 2020. The ... for large scale implementation and review of biometric ... launch of new products with greater efficiency, are ...
Breaking Biology News(10 mins):Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 2Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 3HYPR Corp. Joins the FIDO Alliance 2HYPR Corp. Joins the FIDO Alliance 3Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2
... in Hong Kong this year. So far, 466 children were infected ... Streptococcus pyogenes, a gram-positive pathogen that can be transmitted via ... Hong Kong Center for Disease Control (HK CDC), Hong Kong University ... the S. pyogenes strain that was isolated from a ...
... Va. recently found a way to improve electricity generating ... less expensive. Specifically, they discovered a way to speed ... which are necessary for fuel cells to operate. ... membrane, or PEM, commonly used in fuel cells and ...
... and the USDA Agricultural Research Service have developed a new ... boniato, or tropical sweet potato, Liberty has a dark red ... Boniato potatoes originated in the tropical Americas and are grown ... served fried, mashed or in soup. "We developed Liberty ...
Cached Biology News:Hong Kong University leads the genomics research of scarlet fever pathogen 2Stretching old material yields new results for energy- and environment-related devices 2
Fast, sensitive quantitation of Mouse IgM concentration....
Detect and measure levels of mouse IgG3 antibody isotypes quickly and easily....
Request Info...
ExonBio Service provides one-stop recobinant protein shopping from cloning, expression and purification. Price including gene cloning....
Biology Products: