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Bacterial Whole Genome Array Analysis

treatments:

Immediate cell lysis and RNA purification using RiboPure-Bacteria Kit

Rapid freezing in liquid nitrogen (a freeze-thaw treatment may aid lysis of some bacteria)

Resuspension of cells in RNAlater (see RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis).

These simple steps are of vital importance for obtaining the highest quality RNA required in array analysis.


Isolating Bacterial Total RNA: RiboPure-Bacteria
Traditional methods for isolation of total RNA from bacteria require the use of hot phenol or include other harsh techniques, some of which need specialized equipment. For Gram-positive organisms, pretreatments with enzymes to weaken the cell wall are often used prior to isolation of RNA. However, it should be kept in mind that these enzymatic pretreatments may alter gene expression patterns on a global scale and could compromise array data.

The RiboPure-Bacteria RNA Isolation Kit uses no enzymatic pretreatments and thus will not alter gene expression profiles. RiboPure-Bacteria combines an efficient glass bead and phenol-based RNAWIZ-mediated disruption step followed by a glass fiber filter-based RNA purification step for high yi
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Related biology technology :

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2. Mammalian and Bacterial Expression in One Vector
3. Bacterial mRNA Isolation, Fast and Easy
4. Bacterial RNA Isolation From Infected Eukaryotic Hosts
5. Bacterial RNA Isolation From Infected Eukaryotic Hosts
6. Perform Microarray Analysis with Limited Bacterial RNA
7. RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis
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