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Bacterial Whole Genome Array Analysis

Isolate and Enrich for Bacterial mRNA


Isolating Bacterial RNA for Microarray Analysis
The rapid increase in the number of bacterial genomes sequenced over the past several years has opened up a new era in whole-genome expression analysis using DNA microarray technology. In recent years, this tool has been used to explore transcriptional profiles for a variety of bacteria leading to a greater understanding of microbial metabolism [1]. DNA microarray technology has also led to a better understanding of microbial pathogenesis, physiology, epidemiology, ecology, fermentation, and pathway engineering.

With the emerging importance of bacterial microarray gene expression analysis, it is vital that bacterial RNA isolated for array analysis be of the highest possible quality. Key characteristics unique to bacteria known to significantly impact RNA quality, and dramatically influence the quality and validity of array analysis, include:

1. Growth phase at harvest and harvest conditions

2. Rapid turnover of bacterial mRNA

3. Bacterial mRNAs' lack of a poly(A) tail, thus excluding the use of classical enrichment methods (e.g. oligo(dT) enrichment)

The last of these factors was the basis for developing Ambion's MICROBExpress Kit. MICROBExpress dramatically increa
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