Navigation Links
Bacterial RNA Isolation From Infected Eukaryotic Hosts

e supernatant and can be precipitated with ethanol. Figure 1 shows an Agilent 2100 bioanalyzer electropherogram of a complex host-bacterial RNA population before and after bacterial RNA purification with MICROBEnrich. The RNA profile indicates nearly complete removal (>95%) of the host 18S and 28S rRNAs.

Figure 1. RNA Before and After Enrichment with MICROBEnrich Kit. Rat liver total RNA (25 g) and E. coli total RNA (2 g) were mixed together and subjected to the MICROBEnrich procedure. Another mixed sample of total RNA was subjected to a mock MICROBEnrich procedure in which the capture oligonucleotide mix was left out of the reaction. RNA was precipitated and an equal volume of each sample was separated on an RNA Nano LabChip with the Agilent 2100 bioanalyzer.


Removal of Bacterial rRNA
If desired, upon completion of the MICROBEnrich procedure bacterial mRNA can be purified from the total RNA through the seamless integration of Ambion's MICROBExpress technology. This technology enables >95% removal of prokaryotic 16S and 23S rRNAs (Figure 2). See the article "Purify mRNA From Bacteria!" for information on MICROBExpress.

Figure 2. RNA Before and After Enrichment with MICROBEnrich. Rat liver total RNA (25 g) and E. coli total RNA (2 g) were mixed together and subjected to the MICROBEnrich procedure with or without a further enrichment by MICROBExpress. The total RNA lane was subjected to a mock MICROBEnrich procedure in which the capture olig
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. High-Throughput Cultivation of Bacterial Clones
2. Mammalian and Bacterial Expression in One Vector
3. Bacterial mRNA Isolation, Fast and Easy
4. Bacterial RNA Isolation From Infected Eukaryotic Hosts
5. Bacterial Whole Genome Array Analysis
6. Perform Microarray Analysis with Limited Bacterial RNA
7. RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis
8. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
9. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
10. Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes
11. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Bacterial RNA Isolation From Infected Eukaryotic Hosts

(Date:1/23/2015)... 23, 2015 Portal Solutions , a ... on Microsoft Office 365 and SharePoint platforms , today ... program for being one of the region’s fastest-growing mid-sized companies ... growth. , “To be recognized a second year in ...
(Date:1/22/2015)... , Jan. 22, 2015  Transwestern | RBJ today announces ... of office space for Shire a leading biopharmaceutical company, at ... Transwestern | RBJ,s Robert Richards , president, and ... lease for the entire five-floor building at 95 Hayden Ave. ...
(Date:1/22/2015)... January 22, 2015 Pipette.com has added ... to their comprehensive portfolio of Eppendorf products. , The ... purchase a Centrifuge 5424/5424 R and receive the following:, ... Eppendorf Research plus or Eppendorf Reference 2 , ...
(Date:1/22/2015)... (PRWEB) January 22, 2015 Diagenode, ... such as the Bioruptor® and complete solutions for ... for chromatin immunoprecipitation, alleviating the need for manual ... controls needed for ChIP of histones or transcription ...
Breaking Biology Technology:Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 2Washington SmartCEO Announces Portal Solutions as 2015 Future 50 Award Winner Second Year in a Row 3Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2
... over Internet Protocol, or VoIP, may have found the missing ... a lot of telephone companies go into a state of ... ,The telcos and cable companies are both trying to figure ... voice over the Internet and to be able to connect ...
... YellowTop battery. Courtesy of Optima Batteries. Milwaukee ... Milwaukee-based Johnson Controls , has announced the latest ... introduction of the dual-purpose Group 75/25 and 35 models. ... on vehicles by a growth of in-car electronics. , ...
... magazines recently released list of the top 100 ... A. Graham, vice president and CIO of Church Mutual ... to religious organizations, including churches as well as schools, camps ... 50 states. , , Herman Nell, senior vice president and ...
Cached Biology Technology:The day the old telephony died 2The day the old telephony died 3Computerworld: Three Wisconsin CIOs among top 100 IT leaders 2
(Date:12/11/2014)... 2014  That blood pressure plays a role in human health ... medical term for high blood pressure – was first described ... cuff that,s used in measuring blood pressure was invented in ... about hypertension, its triggers and its effects. In fact, recent ...
(Date:12/10/2014)...  Valencell, a leader in performance biometric data sensor technology, ... its licensees for highly accurate, clinically validated biometric technology. ... from fitness and health sectors, but first responders/military and ... only as useful as the biometric data it delivers. ...
(Date:12/10/2014)... Research and Markets ( http://www.researchandmarkets.com/research/xclcvw/biometrics_market ) has ... Japan 2014-2018" report to their offering. ... of biometrics and smart cards for projects such as ... one of the major trends witnessed in the market. ...
Breaking Biology News(10 mins):Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4Wearable Technology Products Demand Highly Accurate Biometric Technology 2Biometrics Market in Japan 2014-2018: Key Vendors are DDS, Fujitsu, Hitachi and NEC 2
... -- typically a full meter across, with a bud the ... plants whose blossoms are nearly all tiny, botanists write this ... reveals that it is closely related to a family that ... plants, and the tropical root crop cassava. , The team ...
... A surprising technique to concentrate, manipulate, and separate ... identified through a collaboration between researchers at Argonne ... Arizona at Tucson, and Cambridge University, UK. This ... biomedical engineering including use in miniaturized medical diagnostic ...
... an enzyme that responds to a buildup of fatty ... in the biochemical pathway that breaks down these fatty ... Hospital. This breakdown of fatty acids, in turn, provides ... fatty acids will accumulate. , The St. Jude ...
Cached Biology News:World's largest flower evolved from family of much tinier blooms 2World's largest flower evolved from family of much tinier blooms 3Researchers develop technique for bacteria crowd control 2Protein sensor for fatty acid buildup in mitochondria 2
Cyan Standard is 7-hydroxy-4-trifluoromethylcoumarin...
6-(2,4-dinitrophenyl)aminohexanoic acid, succinimidyl ester (DNP-X, SE)...
Endothelin-converting enzyme 1 (EC 3.4.24.71) (ECE-1). [Source:Uniprot/SWISSPROT;Acc:P42892] Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
...
Biology Products: