Navigation Links
Bacterial RNA Isolation From Infected Eukaryotic Hosts

ure. This host-bacterial cell total RNA mixture can be obtained by a variety of RNA isolation methods, ideally by Ambion's new RiboPure-Bacteria Kit. Other methods including glass fiber filter based methods (such as Ambion's RNAqueous Kits) and phenol based methods (such as Ambion's RNAwiz and the ToTALLY RNA Kit) are also appropriate. For microarray analysis and other sensitive applications, Ambion also recommends a DNase treatment (such as with DNAfree Treatment and Removal Reagents) to remove contaminating genomic DNA from the total RNA prior to the MICROBEnrich procedure.


Remove >90% of Mammalian RNA from Complex Total RNA Mixtures

MICROBEnrich employs hybridization capture technology (patent pending) to remove human, mouse, and rat RNA (both mRNA and rRNA) from complex host-bacterial RNA populations, leaving behind enriched microbial total RNA. In the first step of the MICROBEnrich procedure, host-bacterial total RNA is incubated with an optimized mixture of capture oligonucleotides that bind to the mammalian 18S and 28S rRNAs and polyadenylated RNAs. Next, the rRNA/oligo nucleotide hybrids and all polyadenylated mRNAs are removed from the mixture with oligonucleotide-derivatized magnetic beads. After magnetic separation, the bacterial RNA remaining in th
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. High-Throughput Cultivation of Bacterial Clones
2. Mammalian and Bacterial Expression in One Vector
3. Bacterial mRNA Isolation, Fast and Easy
4. Bacterial RNA Isolation From Infected Eukaryotic Hosts
5. Bacterial Whole Genome Array Analysis
6. Perform Microarray Analysis with Limited Bacterial RNA
7. RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis
8. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
9. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
10. Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes
11. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Bacterial RNA Isolation From Infected Eukaryotic Hosts

(Date:3/4/2015)... , March 4, 2015 /CNW/ - Resverlogix ... it has completed a collaborative research program ... analytics.  Using Fast Collective Evolution Technology (FACET), ... including all measurements obtained from each of ... Phase 2 clinical trials ASSERT, SUSTAIN and ...
(Date:3/4/2015)... Proove Biosciences , a ... excited to announce the launch of their CME-accredited ... Management of Pain”. , The continuing medical education ... next year. The focus of the program is ... pain treatments, integrate appropriate pain management techniques, and ...
(Date:3/4/2015)... , March 4, 2015  BioClinica®, Inc., ... technology provider, today announced that clinical trial ... convened at its annual European User Conference ... to discuss the use of its eClinical ... eClinical technologies include: the Microsoft Office-Smart OnPoint ...
(Date:3/4/2015)... 2015 Follow us on LinkedIn ... the fastest growing technologies in the life sciences industry. ... is driving growth in nucleic acid detection technologies like ... gene expressions will continue to benefit the qPCR and ... ranging from pharma, biotech, food & beverage to animal ...
Breaking Biology Technology:Resverlogix Collaborates with Emerald Logic to Identify Factors Driving Drug Response and Efficacy in Phase 2 Program 2Resverlogix Collaborates with Emerald Logic to Identify Factors Driving Drug Response and Efficacy in Phase 2 Program 3Resverlogix Collaborates with Emerald Logic to Identify Factors Driving Drug Response and Efficacy in Phase 2 Program 4Proove Biosciences Launches CME Accredited Program with Medscape 2Proove Biosciences Launches CME Accredited Program with Medscape 3BioClinica Teams With Pharma To Tackle Clinical Trial Challenges In Europe 2BioClinica Teams With Pharma To Tackle Clinical Trial Challenges In Europe 3Focus on Quantifying Nucleic Acid Molecules Spurs Growth in the qPCR & dPCR Instrumentation Market, According to a New Report by Global Industry Analysts, Inc. 2Focus on Quantifying Nucleic Acid Molecules Spurs Growth in the qPCR & dPCR Instrumentation Market, According to a New Report by Global Industry Analysts, Inc. 3Focus on Quantifying Nucleic Acid Molecules Spurs Growth in the qPCR & dPCR Instrumentation Market, According to a New Report by Global Industry Analysts, Inc. 4
... LLC (L3I) has,successfully completed the Quality Management System (QMS) ... ISO 9001 and AS 9100,Certification under the rules of ... compliance and zero non-compliance issues. , ... Operations, Scott McAfee led,the creation, organization and refinement of ...
... Pharmaceuticals Inc.,is pleased to announce the appointment of Barry ... draw upon his 24 years of pharmaceutical sales and,marketing ... products for the company. , ... "We are extremely pleased to welcome Mr. ...
... 13 Johnston Blakely & Company, LLC, a,Boston-based ... that Michael,D. Webb has joined the firm as ... will,participate with the firm on internal and client-related ... equity placements, with a,particular focus on biopharmaceutical transactions. ...
Cached Biology Technology:Level 3 Inspection Achieves 100% Compliance in ISO 9001 & AS 9100 Certification 2
(Date:2/5/2015)... , Feb. 3, 2015 Despite ... demand from industries such as consumer electronics, automotive, ... for surface mount technology (SMT) screen printers. Innovations ... automatic control will push the adoption curve up. ... remain steady as glue dispensers are indispensable in ...
(Date:1/22/2015)... , Jan. 13, 2015  Today, FreeWavz ( www.FreeWavz.com ... its crowdfunding campaign on Fundable, https://www.fundable.com/freewavz . ... of production capacity to meet customer demand. ... - http://photos.prnewswire.com/prnh/20150113/168790 Invented ...
(Date:1/22/2015)... NXT-ID, Inc. (NASDAQ: NXTD and NXTDW) a biometric authentication ... success of the Wocket™ smart wallet at CES 2015 in ... as one of the "11 Hot Products at CES" in a review ... So Far" by Newseveryday.com and "The top 10 gadgets from CES 2015" ...
Breaking Biology News(10 mins):End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 2End-user Demand Spurs Global SMT Screen Printers and Glue Dispensers Market 3FreeWavz Launches on Fundable to Drive Speed-to-Market in 2015 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 3
... headed by Dr. Sara Melville at the University of ... African sleeping sickness has evolved an unusual chromosomal structure ... parasite, the chromosomes are highly enriched in subtelomeric sequences ... to the gene-rich cores. These subtelomeres, in some ...
... the Pacific Northwest Research Institute (PNRI) and the National ... pattern of DNA damage in connective tissues in the ... stages of breast cancer and possibly serve as an ... In the United States, breast cancer is the second ...
... years, researchers have struggled to understand how IVIG worked. ... bean apparent contradiction. , Intravenous immunoglobulin (IVIG) is ... plasma that contains the pooled antibodies from thousands of ... assorted conditions; nonetheless, practitioners have used it off-label with ...
Cached Biology News:DNA to the defense 2Links between DNA damage and breast cancer studied 2A single sugar found responsible for an antibody's ability to treat inflammation 2A single sugar found responsible for an antibody's ability to treat inflammation 3
DDO Immunogen: DDO (NP_003640, 270 a.a. ~ 370 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
Yersinia pestis...
Insect Cell Lysis Buffer 50 ml...
Rabbit polyclonal to Orexin Prepro ( Abpromise for all tested applications). entrezGeneID: 3060 SwissProtID: O43612...
Biology Products: