Navigation Links
Bacterial RNA Isolation From Infected Eukaryotic Hosts

o mix was left out of the reaction. RNA was precipitated and an equal fraction of each sample was analyzed by denaturing agarose gel electrophoresis.


MICROBEnrich Will Work With Any Bacterial Species
MICROBEnrich was optimized using E. coli RNA in a background of human, mouse and rat RNA. However, the kit should perform well with RNA from any bacterial species. Although many mammalian host species may be compatible with MICROBEnrich, only human, mouse and rat rRNA sequences were used for the design and optimization of the capture oligonucleotides.


Increased Sensitivity in Downstream Analyses

Bacterial mRNAs purified with the combined MICROBEnrich and MICROBExpress Kits serve as better templates than total RNA for downstream applications and therefore deliver dramatic increases in sensitivity when used in applications such as array analysis. Figure 3 shows Sigma-Genosys Panorama E. coli arrays that were hybridized with labeled cDNA derived from either a mixed population of total RNA (E. coli/HeLa cell RNA mixture) or mRNA purified from the mixture using the MICROBEnrich and MICROBExpress Kits. The number of transcripts detected with labeled RNA target increases substantially after bacterial mRNA enrichment using the MICROBEnrich and MICROBExpress Kits.

Figure 3. Enrichment for Bacterial mRNA Results in More Sensitive Array Analysis. Replicate portions of Sigma/Genosys E. coli Panorama arrays were hybridized under identical conditions with 33P-labeled cDNA s
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. High-Throughput Cultivation of Bacterial Clones
2. Mammalian and Bacterial Expression in One Vector
3. Bacterial mRNA Isolation, Fast and Easy
4. Bacterial RNA Isolation From Infected Eukaryotic Hosts
5. Bacterial Whole Genome Array Analysis
6. Perform Microarray Analysis with Limited Bacterial RNA
7. RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis
8. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
9. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
10. Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes
11. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Bacterial RNA Isolation From Infected Eukaryotic Hosts

(Date:8/27/2014)... YORK , Aug. 27, 2014 /PRNewswire/ ... research report is available in its catalogue: ... 2014-2018 http://www.reportlinker.com/p02316661/Global-Biodegradable-Packaging-Materials-Market-2014-2018.html ... materials are materials that are readily decomposed ... differs from conventional non-degradable packaging in terms ...
(Date:8/27/2014)... The Global and China Trifluoroacetic Acid Industry is ... of the Trifluoroacetic Acid industry in Global and China. ... industry, including definitions, applications and industry chain structure. Global ... with a focus on history, developments, trends and competitive ... and Chinese situation is also offered. , The report ...
(Date:8/27/2014)... Aug. 27, 2014  Lisa Kulik saw fireworks for ... 4 holiday, thanks to a groundbreaking retinal implant co-invented ... renowned clinician-researcher at the University of Southern California (USC) ... Peoria, Ariz. resident who has retinitis ... of sight. On June 2, she became the first ...
(Date:8/27/2014)... NEW YORK , Aug. 27, 2014 ... of Guggenheim Partners, today announced the hiring of veteran ... and senior equity analyst. Mr. Butler will focus on ... brings our team a wealth of experience and a ... Dante Ferrarie , Senior Managing Director and Head of ...
Breaking Biology Technology:Global Biodegradable Packaging Materials Market 2014-2018 2Global Biodegradable Packaging Materials Market 2014-2018 3Global Biodegradable Packaging Materials Market 2014-2018 4Global Biodegradable Packaging Materials Market 2014-2018 5Global Biodegradable Packaging Materials Market 2014-2018 6Global Biodegradable Packaging Materials Market 2014-2018 7Trifluoroacetic Acid Industry (Asia, Europe, North America, ROW) Analysis Report Now Available at ReportsnReports.com 2USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 2USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 3USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 4USC Eye Institute ophthalmologists implant first FDA-approved Argus II retinal prosthesis in western United States 5Guggenheim Securities Hires Analyst Charles "Tony" Butler to Expand Healthcare Coverage 2
... Emmanuel Labourier, Marianna Goldrick, Ambion, ... that mediate post-transcriptional gene regulation by pairing , ... untranslated region of messenger RNAs and acting , ... and exciting area of research with applications ...
... High Density Oligonucleotide , Microarrays , ... engineered to maximize yields of full-length cDNA , ... 100 ng of input RNA in a single round for GeneChip , ... cRNA synthesis powered by , ...
... , Dmitriy Ovcharenko, Rich Jarvis, ... Ambion, Inc. , , ... is a powerful , experimental ... genes. It is used routinely for gene function analysis, target , ...
Cached Biology Technology:miRNA Expression in White Blood Cells 2miRNA Expression in White Blood Cells 3miRNA Expression in White Blood Cells 4miRNA Expression in White Blood Cells 5Improving RNA Amplification 2Improving RNA Amplification 3Improving RNA Amplification 4Improving RNA Amplification 5Improving RNA Amplification 6Improving RNA Amplification 7Improving RNA Amplification 8Improving RNA Amplification 9Improving RNA Amplification 10Improving RNA Amplification 11Improving RNA Amplification 12Improving RNA Amplification 13Improving RNA Amplification 14Delivering siRNAs to Difficult Cell Types 2Delivering siRNAs to Difficult Cell Types 3Delivering siRNAs to Difficult Cell Types 4Delivering siRNAs to Difficult Cell Types 5Delivering siRNAs to Difficult Cell Types 6Delivering siRNAs to Difficult Cell Types 7
(Date:8/28/2014)... neighbors than traditional cigarettes, but still release toxins ... from USC. , Scientists studying secondhand smoke from ... to harmful particles, with close-to-zero exposure to organic ... metals in second-hand e-cigarette smoke were found to ... high levels of polycyclic aromatic hydrocarbons cancer-causing ...
(Date:8/28/2014)... MD FASEB MARC (Maximizing Access to Research ... for the Genetics Society of America,s 27th Annual ... October 3, 2014 in Pacific Grove, California. ... of students, post doctorates and scientists from underrepresented ... community and to encourage the participation of young ...
(Date:8/28/2014)... that could positively impact the global battle ... thanks to researchers in Penn State,s College ... made from the seaweed-derived food ingredient carrageenan ... provides a woman-initiated, drug-delivery vehicle that can ... infections during unprotected heterosexual intercourse, the researchers ...
Breaking Biology News(10 mins):Second-hand e-cig smoke compared to regular cigarette smoke 2Sensory-tested drug-delivery vehicle could limit spread of HIV, AIDS 2Sensory-tested drug-delivery vehicle could limit spread of HIV, AIDS 3
... better understanding how antimicrobials bind and thereby get inactivated ... University of Illinois may have found a way to ... not a cure, this work has potential as a ... Gerard Wong a professor of materials science and engineering, ...
... nodes can be crucial for spreading low doses of ... as scrapie and Creutzfeldt-Jakob disease - into the nervous ... open access journal BMC Veterinary Research. While we can ... role in the neuroinvasion of prions for high doses ...
... caused the extinction of mammoths and the decline of ... debated. Overhunting by Paleoindians, climate change and disease lead ... considered a little out there is now hitting closer ... including two Northern Arizona University geologists, reports evidence that ...
Cached Biology News:Cystic fibrosis patients may breathe easier, thanks to bioengineered antimicrobials 2Lymph nodes can be key in spreading prion infectivity 2Research team says extraterrestrial impact to blame for Ice Age extinctions 2Research team says extraterrestrial impact to blame for Ice Age extinctions 3
...
... positively and negatively supercoiled DNA in presence of ... the effects of supercoiling on transcription in vitro ... , Determining the degree of supercoiling of naturally ... differ in length by only one base-pair ...
Required to perform reference file generation, flatfield calibration and performance verification processes on the GSD-501...
Applications: Immunohistochemistry, ISH, FISH, CISH....
Biology Products: