Navigation Links
Bacterial RNA Isolation From Infected Eukaryotic Hosts

o mix was left out of the reaction. RNA was precipitated and an equal fraction of each sample was analyzed by denaturing agarose gel electrophoresis.


MICROBEnrich Will Work With Any Bacterial Species
MICROBEnrich was optimized using E. coli RNA in a background of human, mouse and rat RNA. However, the kit should perform well with RNA from any bacterial species. Although many mammalian host species may be compatible with MICROBEnrich, only human, mouse and rat rRNA sequences were used for the design and optimization of the capture oligonucleotides.


Increased Sensitivity in Downstream Analyses

Bacterial mRNAs purified with the combined MICROBEnrich and MICROBExpress Kits serve as better templates than total RNA for downstream applications and therefore deliver dramatic increases in sensitivity when used in applications such as array analysis. Figure 3 shows Sigma-Genosys Panorama E. coli arrays that were hybridized with labeled cDNA derived from either a mixed population of total RNA (E. coli/HeLa cell RNA mixture) or mRNA purified from the mixture using the MICROBEnrich and MICROBExpress Kits. The number of transcripts detected with labeled RNA target increases substantially after bacterial mRNA enrichment using the MICROBEnrich and MICROBExpress Kits.

Figure 3. Enrichment for Bacterial mRNA Results in More Sensitive Array Analysis. Replicate portions of Sigma/Genosys E. coli Panorama arrays were hybridized under identical conditions with 33P-labeled cDNA s
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. High-Throughput Cultivation of Bacterial Clones
2. Mammalian and Bacterial Expression in One Vector
3. Bacterial mRNA Isolation, Fast and Easy
4. Bacterial RNA Isolation From Infected Eukaryotic Hosts
5. Bacterial Whole Genome Array Analysis
6. Perform Microarray Analysis with Limited Bacterial RNA
7. RNAlater Preserves Bacterial Gene Expression Profiles for Array Analysis
8. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
9. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
10. Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes
11. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Bacterial RNA Isolation From Infected Eukaryotic Hosts

(Date:11/26/2014)... YORK, NOVEMBER 21, 2014 (PRWEB) November 26, 2014 ... largest health-oriented communications network, today announced the organization has ... Annual Rx Club Awards. Digitas Health LifeBrands was honored ... while four other PHCG agencies received eight Awards of ... the creative aspects of pharmaceutical advertising and promotion. The ...
(Date:11/26/2014)... 26, 2014 The North America ... the market for detailed analysis of the growth ... to reach $616.3 million by 2018, growing at ... , Browse through the TOC of the North ... an idea of the in-depth analysis and industry ...
(Date:11/26/2014)... , Nov. 26, 2014 Space is one ... of space requires astronauts to maintain consistently high levels ... and prevent potential errors and accidents. Despite the importance ... about how cognition is affected by prolonged spaceflight, and ... Now, Penn Medicine researchers are poised to help the ...
(Date:11/26/2014)... The ETC (Emerging Technology Centers) ... announced today that applications for AccelerateBaltimore™ (AB) 2015 ... you have a big idea?,” asked ETC’s President ... about it. AccelerateBaltimore helps you and your big ... 13 weeks.” Interested game changers with “big ...
Breaking Biology Technology:Publicis Healthcare Wins 20 Rx Club Awards 2Publicis Healthcare Wins 20 Rx Club Awards 3The North America Thermal Protective Clothing Market is estimated to grow up to $616.3 million by 2018 - Report by MicroMarket Monitor 2The North America Thermal Protective Clothing Market is estimated to grow up to $616.3 million by 2018 - Report by MicroMarket Monitor 3Penn Medicine Team Develops Cognitive Test Battery to Assess the Impact of Long Duration Spaceflights on Astronauts' Brain Function 2Penn Medicine Team Develops Cognitive Test Battery to Assess the Impact of Long Duration Spaceflights on Astronauts' Brain Function 3Penn Medicine Team Develops Cognitive Test Battery to Assess the Impact of Long Duration Spaceflights on Astronauts' Brain Function 4AccelerateBaltimore Applications to Close in 6 Days 2AccelerateBaltimore Applications to Close in 6 Days 3
... Back Full Control of Belinostat, Consolidating ... the Global Rights for the Product TopoTarget ... , - Deal Covers a Payment of USD 26 million (DKK ... Payment, - Webcast Conference Call to be Held 2pm CET, ...
... Calif., April 21 Ortho Evra is an adhesive,transdermal birth ... and 20 mcg of ethinyl estradiol into the bloodstream per ... and no patch is worn,during the fourth week. Ortho Evra ... million women have used Ortho Evra since its approval.,Ortho Evra ...
... Pharmacyclics,Inc. (Nasdaq: PCYC ) today announced that ... 17, 2008 indicating that, for the last 30,consecutive ... has closed,below the minimum bid price of requirement ... Nasdaq Global Market under Marketplace Rule 4450(a)(5). In,accordance ...
Cached Biology Technology:TopoTarget Successfully Buys Back Full Control of Belinostat 2TopoTarget Successfully Buys Back Full Control of Belinostat 3TopoTarget Successfully Buys Back Full Control of Belinostat 4TopoTarget Successfully Buys Back Full Control of Belinostat 5TopoTarget Successfully Buys Back Full Control of Belinostat 6TopoTarget Successfully Buys Back Full Control of Belinostat 7Ortho Evra Patch Makers Settling Cases 2
(Date:11/21/2014)... DALLAS , November 18, 2014 ... Security Systems Market by Systems (Video, RFID, Access Control, ... (SEZ, Factories, Hotels, Banks, Government), Component Service Geography - ... Security Systems Market is projected to be around $25 ... Billion by 2020, growing at a CAGR of 8.69%. ...
(Date:11/21/2014)... NEW YORK , Nov. 19, 2014  Earlier ... Board at New York College, and one of the ... for biosensor signals that are transmitted from Smartphones to ... there were Smartphones and has one of the earliest ... are discussing its usage in the military, child care, ...
(Date:11/21/2014)... , Nov. 20, 2014 Strict laws against ... are piloting the North American and European automotive sector ... growing, gesture recognition systems that are intuitive and able ... in the industry. New analysis from ... Recognition Market in Europe and ...
Breaking Biology News(10 mins):Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 2Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 4
... been shed on proteorhodopsin, the light-sensitive protein found in ... of Energy's Lawrence Berkeley National Laboratory (Berkeley Lab) and ... when the ability to respire oxygen is impaired, bacterium ... carry out vital life processes. , ...
... an increased risk of fetal heart problems when mothers ... an abstract presented by Yale School of Medicine researchers ... in San Francisco. , Congenital heart block (CHB) ... heart's electrical signaling from the upper to the lower ...
... system to produce antibodies that act against some essential ... can act against sperm, eggs or reproductive hormones, which ... ova. , Proponents of the technique, which ... as more humane than the conventional methods of controlling ...
Cached Biology News:Shedding new light on proteorhodopsin 2Shedding new light on proteorhodopsin 3Manipulating nature: Scientists query wildlife birth-control method 2
... Chromatograph/Mass Spectrometer, utilizing many of the ... offers high throughput and excellent productivity, ... ratio. Like the GCMS-QP2010 Plus, the ... for optimum separation, 20 temperature ramps, ...
... XS uniquely combines a single-channel sample processing ... 8-channel bulk reagent dispenser all in one ... using disposable pipette tips, the Precision XS ... dispenser is available for transferring larger volumes. ...
... As the world of discovery races ever faster, ... on the fast track. So our constant focus ... accelerate your research. Thats why our Biomek 3000 ... thats as advanced as it is flexible. By ...
... MaxArray Rabbit Tissue Arrays contain 60 rabbit tissue ... package contains 4 unstained slides and 1 hematoxylin ... Each slide contains 15 different tissues from 4 ... samples per slide. Each tissue sample (or core) ...
Biology Products: