( Remove 90% of mamma...)Bacterial RNA Isolation From Infected Eukaryotic Hosts

Simple procedure takes less than 2 hours

Works with any bacterial species

Seamless integration with MICROBExpress Bacterial mRNA Purification Kit

Enables microarray expression analysis with bacterial RNA isolated from host cells

Ambion's new MICROBEnrich (patent pending) is the only kit available for bacterial RNA enrichment from mixed host-bacterial RNA populations. A robust yet simple procedure, MICROBEnrich depletes mammalian (host cell) RNA selectively, leaving behind highly enriched bacterial RNA.

Gene expression analysis following host-bacterial interactions is revolutionizing our understanding of the host response to bacterial infection. Numerous studies have utilized microarray analysis to follow host cell transcriptome alterations in response to interactions with bacteria. However, because no method has existed to isolate bacterial RNA away from host cell RNA, the corresponding analyses of bacterial transcriptomes has been impossible. Ambion now introduces a new technology, MICROBEnrich, that enables whole genome microarray expression analyses of bacterial RNA after host-bacterial interactions.

Start With Any Total RNA Sample
Although the mass of eukaryotic RNA isolated from host-bacterial cell mixtures is often far greater than the mass of bacterial RNA isolated, this complex mixture of total RNAs is the starting material for the MICROBEnrich proced
'"/>

Source: