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BD IMag Human Regulatory T Lymphocyte Separation Set for Isolation of Treg Cells

BD Biosciences is pleased to introduce the BD IMag Human Regulatory T Lymphocyte Separation Set DM (Cat. No. 558142) for the isolation of Treg cells from peripheral blood mononuclear cells (PBMC). The reagent set isolates Treg cells in two stages. First, negative selection is performed using BD IMag streptavidin particles with a cocktail of biotinylated monoclonal antibodies that recognizes antigens expressed on erythrocytes, platelets, granulocytes, monocytes, B cells, and CD8 T cells to enrich for the CD4 T lymphocytes. Then, the CD25+ CD4+ Treg cells are isolated from the enriched CD4 T lymphocytes by positive selection. This Enrichment Set has been optimized for use with the BD IMagnet, and it contains sufficient reagents to label 109 PBMC.

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