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BALB/3T3

/ml final concentration, in bidistilled H2O).
  • Transfer 400 l cell suspension into electroporation cuvettes (2 mm gap width). The cell suspension must be free of air bubbles.
  • Electroporation:

    Mode Eukaryotes Voltage (V) 720 V Time constant (T) 40 s No. of pulses (n) 1
  • After the pulse, allow the cell suspension to stand in the cuvette for 5-10 minutes at room temperature.
  • Carefully transfer the cell suspension from the cuvette to 3 ml DMEM / 10% FCS, and cultivate them in a 55 mm culture dish.
  • Detection methods for transfection:
    The expression of the plasmid pEGFP-N1 can be detected clearly after 24-48 hours with the aid of FACS analysis or under a fluorescence microscope. Result: Survival rate: 125% Transfection rate: 28% based on the initial number of cells used for the experiment Results were measured 48 hours after transfection.


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