The population of siRNAs produced by the Silencer siRNA Cocktail Kit (RNase III) elicits gene silencing effects comparable to well designed specific siRNAs (Figure 2). Ambion scientists have tested many genes and they have yet to see an increase in cytotoxicity nor have they seen any nonspecific effects associated with the use of siRNA populations as compared to individual chemically synthesized siRNAs targeting the same gene (3).
Complete Kit for siRNA Cocktail Production
The Silencer siRNA Cocktail Kit (RNase III) includes reagents to synthesize long dsRNAs by in vitro transcription with T7 RNA polymerase, and to digest these dsRNAs into siRNA cocktails using RNase III. Reagents are provided to prepare siRNA cocktails to 20 different genes, with each RNase III reaction and subsequent purification producing enough siRNA for over a hundred transfections in 24 well plates. siRNA Purification Units are also available separately.
Figure 1.Gene Silencing with the Silencer siRNA
Cocktail Kit A population of siRNAs targeting 200 nt of the Ku-70 mRNA
was prepared with the Silencer siRNA Cocktail Kit (RNase III) and
transfected into HeLa cells at a final concentration of 100 nM. Cells
were analyzed 48 hours later by immunofluorescence. Ku-70 levels were