Navigation Links
Avoid siRNA Design Altogether!

No more siRNA design and testing

Entire procedure complete in 1 day

Includes reagents for long dsRNA preparation, RNase III digestion and siRNA cleanup

Simplify Your Gene Silencing Experiments
The use of small interfering RNAs (siRNAs) to induce targeted gene silencing in mammalian cells is now a common method for analyzing gene function. One drawback to the technology, however, is the need to design, synthesize, and test several siRNAs before an effective sequence is identified. This involves considerable time and expense. Now the "siRNA design problem" can be avoided altogether. With the Silencer siRNA Cocktail Kit (RNase III), researchers can prepare a population of siRNAs to a specific target in a quick, simple procedure. This population of siRNAs elicits gene silencing comparable to validated individual siRNAs without the need to design and test individual siRNA sequences.

Preparing siRNA Cocktails
Recently it has been shown that a population of siRNAs generated by digesting long dsRNA with RNase III can be used to effectively induce RNAi in mammalian systems (14). RNase III cleaves dsRNA into 1230 bp dsRNA fragments with 2 to 3 nucleotide 3' overhangs, and 5'-phosphate and 3'-hydroxyl termini. The termini and overhangs of RNase III cleavage products are thus the same as those produced by Dicer in the in vivo RNAi pathway.

The population of siRNAs produced by the Silencer siRNA Cocktail Kit (RNase III) elicits gene silencing effects comparable to well designed specific siRNAs (Figure 2). Ambion scientists have tested many genes and they have yet to see an increase in cytotoxicity nor have they seen any nonspecific effects associated with the use of siRNA populations as compared to individual chemically synthesized siRNAs targeting the same gene (3).

Complete Kit for siRNA Cocktail Production
The Silencer siRNA Cocktail Kit (RNase III) includes reagents to synthesize long dsRNAs by in vitro transcription with T7 RNA polymerase, and to digest these dsRNAs into siRNA cocktails using RNase III. Reagents are provided to prepare siRNA cocktails to 20 different genes, with each RNase III reaction and subsequent purification producing enough siRNA for over a hundred transfections in 24 well plates. siRNA Purification Units are also available separately.

Figure 1.Gene Silencing with the Silencer siRNA Cocktail Kit A population of siRNAs targeting 200 nt of the Ku-70 mRNA was prepared with the Silencer siRNA Cocktail Kit (RNase III) and transfected into HeLa cells at a final concentration of 100 nM. Cells were analyzed 48 hours later by immunofluorescence. Ku-70 levels were reduced 86% in cells transfected with the siRNA cocktail, compared to non-transfected controls.

Figure 2. siRNA Cocktails Are as Effective as Individual siRNAs A population of siRNAs targeting the first 200 bases downstream of the AUG start site of GAPDH mRNA was prepared with the Silencer siRNA Cocktail Kit (RNase III). The resulting siRNA cocktail, or a chemically synthesized siRNA known to efficiently silence GAPDH, was transfected into HeLa cells. GAPDH protein levels were analyzed by immunofluorescence 48 hrs later.

back to top

Ordering Information Cat# Product Name Size 1920 RNAqueous-96 Kit 192 purifications


Page: All 1 2 3

Related biology technology :

1. Custom and library siRNA for efficient gene silencing
2. Custom and library siRNA for efficient gene silencing
3. Cancer siRNA Oligo Set Version 1.0
4. Library siRNA
5. Custom siRNA Oligo Synthesis Service
6. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
9. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
10. siRNA transfection optimization with the Agilent 2100 bioanalyzer
11. siRNA Design Guidelines
Post Your Comments:

(Date:11/24/2015)... -- Halozyme Therapeutics, Inc. (NASDAQ: HALO ) will be presenting ... on Wednesday, December 2 at 9:30 a.m. ET/6:30 a.m. ... will provide a corporate overview. th Annual Oppenheimer ... p.m. ET/10:00 a.m. PT . Jim Mazzola , vice ... overview. --> th Annual Oppenheimer Healthcare Conference in ...
(Date:11/24/2015)... CITY , Nov. 24, 2015 /PRNewswire/ - Aeterna ... request of IIROC on behalf of the Toronto Stock ... news release there are no corporate developments that would ... --> --> About ... . --> Aeterna Zentaris is ...
(Date:11/24/2015)... ... November 24, 2015 , ... The Academy of Model ... Group (SIG), MultiGP, also known as Multirotor Grand Prix, to represent the First–Person View ... years. Many AMA members have embraced this type of racing and several new model ...
(Date:11/24/2015)... 24, 2015 /PRNewswire/ - Aeterna Zentaris Inc. (NASDAQ: ... the remaining 11,000 post-share consolidation (or 1,100,000 pre-share ... "Series B Warrants") subject to the previously disclosed ... 23, 2015, which will result in the issuance ... to the issuance of such shares, there will ...
Breaking Biology Technology:
(Date:10/26/2015)... ALTO, Calif. and LAS VEGAS ... Nok Nok Labs , an innovator in modern ... Alliance , today announced the launch of its latest ... first unified platform enabling organizations to use standards-based authentication ... The Nok Nok S3 Authentication Suite is ideal for ...
(Date:10/23/2015)... 23, 2015 Research and Markets ( ... Voice Recognition Biometrics Market 2015-2019" report to their ... The global voice recognition biometrics market to grow ... --> --> The report, ... based on an in-depth market analysis with inputs from ...
(Date:10/22/2015)... AWRE ), a leading supplier of biometrics software and services, ... 2015.  --> --> Revenue ... of 33% compared to $6.0 million in the same quarter last ... million, or $0.10 per diluted share, which compared to $2.6 million, ... ago.  --> --> Lower ...
Breaking Biology News(10 mins):