Navigation Links
Avoid siRNA Design Altogether!


No more siRNA design and testing

Entire procedure complete in 1 day

Includes reagents for long dsRNA preparation, RNase III digestion and siRNA cleanup


Simplify Your Gene Silencing Experiments
The use of small interfering RNAs (siRNAs) to induce targeted gene silencing in mammalian cells is now a common method for analyzing gene function. One drawback to the technology, however, is the need to design, synthesize, and test several siRNAs before an effective sequence is identified. This involves considerable time and expense. Now the "siRNA design problem" can be avoided altogether. With the Silencer siRNA Cocktail Kit (RNase III), researchers can prepare a population of siRNAs to a specific target in a quick, simple procedure. This population of siRNAs elicits gene silencing comparable to validated individual siRNAs without the need to design and test individual siRNA sequences.


Preparing siRNA Cocktails
Recently it has been shown that a population of siRNAs generated by digesting long dsRNA with RNase III can be used to effectively induce RNAi in mammalian systems (14). RNase III cleaves dsRNA into 1230 bp dsRNA fragments with 2 to 3 nucleotide 3' overhangs, and 5'-phosphate and 3'-hydroxyl termini. The termini and overhangs of RNase III cleavage products are thus the same as those produced by Dicer in the in vivo RNAi pathway.

The population of siRNAs produced by the Silencer siRNA Cocktail Kit (RNase III) elicits gene silencing effects comparable to well designed specific siRNAs (Figure 2). Ambion scientists have tested many genes and they have yet to see an increase in cytotoxicity nor have they seen any nonspecific effects associated with the use of siRNA populations as compared to individual chemically synthesized siRNAs targeting the same gene (3).


Complete Kit for siRNA Cocktail Production
The Silencer siRNA Cocktail Kit (RNase III) includes reagents to synthesize long dsRNAs by in vitro transcription with T7 RNA polymerase, and to digest these dsRNAs into siRNA cocktails using RNase III. Reagents are provided to prepare siRNA cocktails to 20 different genes, with each RNase III reaction and subsequent purification producing enough siRNA for over a hundred transfections in 24 well plates. siRNA Purification Units are also available separately.

Figure 1.Gene Silencing with the Silencer siRNA Cocktail Kit A population of siRNAs targeting 200 nt of the Ku-70 mRNA was prepared with the Silencer siRNA Cocktail Kit (RNase III) and transfected into HeLa cells at a final concentration of 100 nM. Cells were analyzed 48 hours later by immunofluorescence. Ku-70 levels were reduced 86% in cells transfected with the siRNA cocktail, compared to non-transfected controls.

Figure 2. siRNA Cocktails Are as Effective as Individual siRNAs A population of siRNAs targeting the first 200 bases downstream of the AUG start site of GAPDH mRNA was prepared with the Silencer siRNA Cocktail Kit (RNase III). The resulting siRNA cocktail, or a chemically synthesized siRNA known to efficiently silence GAPDH, was transfected into HeLa cells. GAPDH protein levels were analyzed by immunofluorescence 48 hrs later.


back to top


Ordering Information Cat# Product Name Size 1920 RNAqueous-96 Kit 192 purifications
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Custom and library siRNA for efficient gene silencing
2. Custom and library siRNA for efficient gene silencing
3. Cancer siRNA Oligo Set Version 1.0
4. Library siRNA
5. Custom siRNA Oligo Synthesis Service
6. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
9. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
10. siRNA transfection optimization with the Agilent 2100 bioanalyzer
11. siRNA Design Guidelines
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Avoid siRNA Design Altogether

(Date:7/29/2014)... Calif. , July 29, 2014 Human ... and therapeutic company focused on extending the healthy, high ... Franz Och , Ph.D., an expert in machine ... Scientist. Och comes to HLI from Google where he ... Translate. He will report directly to HLI Co-Founder and ...
(Date:7/29/2014)... (July 29, 2014) Tough, ultralight foam of ... shape through a chemical process invented at Rice University. ... looks like a nanoscale building, with floors and walls ... pair of two-dimensional materials: floors and walls of graphene ... nitride platelets. , The researchers say the foam ...
(Date:7/29/2014)... CITY, Md. , July 29, 2014 /PRNewswire/ ... announced a new and updated program for the ... 10 – 12, 2014 in Amelia Island ... CROs, sites and investigators remains unchanged, however, the ... purposeful exchange and networking opportunity among all stakeholders ...
(Date:7/29/2014)... 2014 Second generation biofuels are a viable ... fuels. However, as natural gas is very cheap, this is ... not as valuable as they could be in comparison to ... projects, has made it difficult for renewable energy developers to ... the ground. For these reasons, certain companies have shifted their ...
Breaking Biology Technology:Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 2Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 3Tough foam from tiny sheets 2TransCelerate, Lilly, Pfizer, and FDA Highlight Collaborative Program at the Site Solutions Summit 2TransCelerate, Lilly, Pfizer, and FDA Highlight Collaborative Program at the Site Solutions Summit 3Renewable Waste Intelligence: Could Renewable Chemicals Be the Future for the Renewable Energy Industry? 2
... 5 Celator Pharmaceuticals today,announced that Andrew Janoff, ... the company,s business operations and clinical development,programs at ... Investor Conference on Tuesday, February 12, 2008 at ... About Celator, Celator Pharmaceuticals, Inc., is ...
... RICHMOND, Va., Feb. 5 Insmed Inc. (Nasdaq:,INSM) CEO ... House FY09 Budget and Food and Drug Administration statements ... is very pleased to see that the White House ... to,deliver safe and more affordable medicines to Americans., ...
... BioSeek, Inc. today,announced that Roy A. Whitfield has ... directors. Mr. Whitfield had joined BioSeek,s board of,directors ... Company since November,2005., "Roy,s management experience and ... extraordinarily valuable to BioSeek thus,far. We look forward ...
Cached Biology Technology:Insmed CEO Issues Statement on White House FY09 Budget and FDA Statements on Follow-On Biologics 2BioSeek Elects Roy Whitfield Executive Chairman of Board of Directors 2
(Date:7/28/2014)... additional coral communities showing signs of damage from the Deepwater ... spill in the Gulf of Mexico. The discovery was made ... at Penn State University. A paper describing this work and ... of Mexico will be published during the last week of ... Proceedings of the National Academy of Sciences . , ...
(Date:7/28/2014)... Essigmann and colleagues from the University of Washington had a ... could induce the virus to mutate uncontrollably, they could force ... that our immune system uses against many viruses. , ... mutate at an enhanced rate, as expected. But it did ... trial reported in 2011. In a new study, however, Essigmann ...
(Date:7/28/2014)... Alzheimer,s disease and other forms of dementia, but the ... , University of Washington bioengineers have a designed a ... the body,s normal proteins into a state that,s linked ... Type 2 diabetes and Lou Gehrig,s disease. The synthetic ... normal state into an abnormally folded form by targeting ...
Breaking Biology News(10 mins):Impact of Deepwater Horizon oil spill on coral is deeper and broader than predicted 2Forced mutations doom HIV 2Forced mutations doom HIV 3Forced mutations doom HIV 4New protein structure could help treat Alzheimer's, related diseases 2New protein structure could help treat Alzheimer's, related diseases 3
... State University scientists and practitioners is riding herd on ... billion cattle industry: Bovine Respiratory Disease. BRD is ... United States, accounting for approximately 75 percent of feedlot ... feedlot deaths. BRD causes between $800 million to $900 ...
... (March 5, 2010) Research presented by P&G Beauty ... the American Academy of Dermatology (Miami Beach, FL, March ... insights into how ingredient formulations, care regimens and gene ... discussing a wide range of topics, including lipid metabolism ...
... (March 4, 2010) -- In a leap toward making stem ... Institute at Weill Cornell Medical College have discovered that endothelial ... produce growth factors that can grow copious amounts of adult ... Until now, adult stem cell cultures would die within four ...
Cached Biology News:Bovine respiratory disease 2P&G Beauty & Grooming presents advancements in skin care at AAD Annual Meeting 2P&G Beauty & Grooming presents advancements in skin care at AAD Annual Meeting 3P&G Beauty & Grooming presents advancements in skin care at AAD Annual Meeting 4Breakthrough reveals blood vessel cells are key to growing unlimited amounts of adult stem cells 2Breakthrough reveals blood vessel cells are key to growing unlimited amounts of adult stem cells 3Breakthrough reveals blood vessel cells are key to growing unlimited amounts of adult stem cells 4Breakthrough reveals blood vessel cells are key to growing unlimited amounts of adult stem cells 5Breakthrough reveals blood vessel cells are key to growing unlimited amounts of adult stem cells 6
... is ideal for the ... (1D electrophoresis, 2D electrophoresis, ... Pharmacokinetics & Toxicology (whole ... chromatography); Physical and Material ...
This kit is designed to select cells labeled with fluorescein isothiocyanate- (FITC-) conjugated antibodies. Kit includes reagents for labeling 3 x 10 9 cells and EasySep magnet....
Fujifilm BAS-1800II provides the ideal configuration for a low-cost imager that requires only a small amount of space in the laboratory. The small imager for accurate high-throughput screening...
When added to either StemSep CD4+ or CD8+ T Cell Enrichment cocktails, human HLA-DR TAC is designed to deplete human HLA-DR from samples to isolate resting CD4+ or CD8+ T cells....
Biology Products: