Navigation Links
Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000

e cycle-sequencing extension products were purified by precipitation with EDTA and 95% high purity ethanol followed by one wash with 70% high purity ethanol. The purified samples were resuspended in 8 μL of 0.1x TE and loaded on an ABI PRISM 3700 DNA Analyzer using the default run modules for POP-5 polymer and an injection time of 90 seconds. Data analysis was performed using Phred base-calling software (CodonCode Corporation, version 0.020425.c) and passing scores were determined to be those samples with greater than 100 Phred Q20 bases, shown in Figure 3. The sequencing results are shown in Table 3.

Discussion

Initial experiments indicate that the Eppendorf Perfectprep BAC 96 kit can be readily automated on the Sciclone ALH 3000. Results are reproducible from plate to plate and high quality DNA is obtained resulting in average sequencing read lengths > 590 bp with 96% pass rates. The versatile Sciclone ALH 3000, using an innovative gripping module, a leading-edge Positive Pressure Filtration system, an on-board shaker module, and a 96-tip head, proves to be an efficient system to obtain 1-16 plates of high-quality BAC DNA in a day. For higher throughput laboratories, a Twister II Plate Handler (CaliperLS) can be incorporated into the system, and scheduled though the CLARA Software (CaliperLS) to provide a higher capacity and throughput system based on the needs. This combination of the easy-to-use Eppendorf Perfectprep BAC 96 kit and the Caliper Life Sciences Sciclone ALH 3000 provide sufficient quantities of BAC DNA suitable for all downstream applications necessary for assembly of large-genome constructs.


'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. RNAqueous-96 RNA Isolation Kit Automation Protocol
2. Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor
3. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
4. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
5. Eppendorf UVette Material Compatibility
6. Eppendorf Perfectprep Gel Cleanup Kit
7. Eppendorf Perfectprep Gel Cleanup Kit
8. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
9. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
10. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
11. EppendorfPerfect gDNA Blood Mini Kit
Post Your Comments:
(Date:1/15/2014)... A study has been launched to test ... track could help to tackle the problem of obesity.  This ... GP surgery based in Stowmarket) and academics at University Campus ... Telemetry technology, which is inspired by equipment used to collect ...
(Date:1/15/2014)... DTS Language Services, Inc . is ... for Life Science organizations who need document translations. Clients ... of their documents in advance with a selection of nearly ... translations, often a critical factor in clinical and scientific fields, ...
(Date:1/15/2014)... 15, 2014 TaiGen Biotechnology Company, Limited ("TaiGen") today ... R-Pharm, a leading Russian pharmaceutical company, to develop and ... Russian Federation , Turkey ... is a novel antibiotic for the treatment of bacterial ...
(Date:1/14/2014)... Communications, a leading provider of strategic communications services to corporations and ... the United States and Europe ... JD, is returning to the firm,s Washington, D.C. ... more than two years of service as Associate Commissioner for the ...
Breaking Biology Technology:Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 2Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 4DTS Improves Efficiency for Life Science Document Translations 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4Former FDA Associate Commissioner Returns To 3D Communications 2
... Benefit From Hidden Scar Surgical Technique ... Performed Through the Belly Button, ... successful completion of what are believed to be the,first ever ... by Dr. Jeffrey Cadeddu, associate professor of,urology and radiology at ...
... Hotel in New York on Thursday, ... ... (Nasdaq:,FVRL), a biopharmaceutical company developing patient-specific, active,immunotherapies for the treatment of ... Officer, will present at,the NewsMakers in the Biotech Industry investment conference ...
... DNA sequencing equipment market,is expected to grow nearly ... million to $528 million as competition intensifies and ... a new report by,Kalorama Information. The DNA ... last,several years, with one dominant vendor and relatively ...
Cached Biology Technology:Novare Announces First Ever Single Port Laparoscopic Kidney Removal (Nephrectomy) Using RealHand(TM) HD Instruments 2Favrille CEO to Present at NewsMakers in the Biotech Industry Investment Conference 2DNA Sequencing Equipment Market to Fragment and Grow as Competition, New Technologies Develop 2
(Date:7/9/2014)... With over 100 diseases that can attack soybean crops, ... the most wanted list? University of Illinois scientists cite ... research is needed on the fungus that causes charcoal ... damp growing conditions but Macrophomina phaseolina , the ... drought conditions. , "As the climate continues to change ...
(Date:7/9/2014)... with pain and fever but often can be defeated with ... and hard to beat. Now, scientists have built a new ... pyramids. Published in the journal ACS Applied Materials & ... and kill more of them than medicine alone. , David ... can lie in wait, undetectable in the human body or ...
(Date:7/9/2014)... may be on the verge of an economy driven by ... than coal and is undergoing a production boom. It has ... recent research is casting serious doubts over just how climate-friendly ... Engineering News (C&EN), the weekly news magazine of the ... senior correspondent at C&EN, explains that when burned as a ...
Breaking Biology News(10 mins):Climate change provides good growing conditions for charcoal rot in soybeans 2Climate change provides good growing conditions for charcoal rot in soybeans 3
... have identified 23 new longevity genes by screening the ... used as a model organism in genetics studies. The ... Genetics. , Each of the 23 genes somehow normally ... them increases lifespan. For example, the worm's lifespan doubles ...
... Unlike most mammals, cats--both domestic and wild--are indifferent ... explains the molecular mechanism behind their strictly carnivorous ... it molecularly," says Joseph Brand, senior author of ... Chemical Senses Center. , Scattered on mammalian tongues ...
... invaluable decision-making tools for public health officials. As ... 2001, models can be useful in two ways: ... infection and they can allow the comparison of ... implicit assumptions that may systematically bias their predictions, ...
Cached Biology News:Field of beams - Novel system uses polarized light pulses to reveal crop health 2
... This CLS number is a ... easily match Cornings product number. ... please order under the old ... customer service for assistance. ID ...
... The Yeast Oligo Microarray Kit (V2), ... to simultaneously characterize expression of many ... gene activity during important biological processes. ... representing 6,256 known ORFs from the ...
... software offers an intuitive, easy-to-use ... control, diverse data handling, and ... Comprehensive functions include data acquisition, ... management in both standalone and ...
... This CLS number is a ... easily match Cornings product number. ... please order under the old ... customer service for assistance. ID ...
Biology Products: