Navigation Links
Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000

clone ALH 3000 to provide an automated 96 well BAC purification system.

Materials and Methods

Bacterial Culture Growth

1.5 mL of 2x YT growth medium was distributed into each well of a 96-well plate then inoculated with BAC clones from CITB Human BAC library plate #707. Four plates from the same quadrant were grown as replicates. The culture plates were incubated at 37 C for 24 hours while shaking at 325 rpm. The bacterial cells were harvested by centrifugation for 10 minutes at 1,900 x g.

Automated Workstation

- Sciclone Advanced Liquid Handler ALH 3000, (CaliperLS) with a High Volume Head (volumes up to 200 μL).
- Deck-mounted self-centering Microplate Shaker (CaliperLS) facilitates the resuspension of the pellet, mixing of the lysate and neutralization solution.
- Positive Pressure Filtration Accessory (CaliperLS) performs the filtration to clear the bacterial lysate, trap the BAC DNA and elute the BAC DNA.
- Gripper (CaliperLS) removes Filter Plate A, & transfer the BAC Filter Plate to the collection plate.
- Wide Bore Tips, 200 μL (Axygen, P/N ZT-205-WB-L-R) are used for the resuspension, lysis, neutralization and the transfer to Filter Plate A.
- Automation Certified Pipette Tips, 200 μL (P/N 56362) are used for pipetting of trapping, wash and elution reagents.

Automated Sample Processing

Sample processing is performed in quadruplicate using the Perfectprep BAC 96 kit on the Sciclone ALH 3000. Initially the reagents, sample plate, filtration plates and pipette tips are placed on the deck as shown on the Layout in the Sciclone software. As the automated sample processing starts, Solution 1 is added to the sample plate (located on the microplate shaker) using wide bore tip
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. RNAqueous-96 RNA Isolation Kit Automation Protocol
2. Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor
3. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
4. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
5. Eppendorf UVette Material Compatibility
6. Eppendorf Perfectprep Gel Cleanup Kit
7. Eppendorf Perfectprep Gel Cleanup Kit
8. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
9. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
10. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
11. EppendorfPerfect gDNA Blood Mini Kit
Post Your Comments:
(Date:5/28/2015)... 2015 Natera, Inc., a leader in ... in Ultrasound in Obstetrics & Gynecology ... prenatal test (NIPT) can identify a complete molar ... A study based upon data collected in the ... about 1 in 1,000 pregnancies, although other studies ...
(Date:5/28/2015)... 2015 RXi Pharmaceuticals Corporation (NASDAQ: RXII ... innovative therapeutics, primarily in the areas of dermatology and ... of 26 million units at a price to the ... approximately $10.4 million. Each unit consists of (i) one ... right to purchase 0.50 of a share of common ...
(Date:5/28/2015)... May 28, 2015 The Fertility Centers ... own Dr. Danielle Vitiello has taken on a new ... Dr. Vitiello will provide guidance, leadership, oversight and quality ... count Dr. Vitiello among our team of experienced reproductive ... CEO, Joseph A. Hill, M.D. “Her expertise and compassionate, ...
(Date:5/28/2015)... 2015 American Process Inc. (Atlanta, ... TN) recently signed a joint agreement ... resins using nanocellulose, a rapidly emerging high performance, ... is the limited number of feedstock materials available. ... to printed parts for load bearing applications. ...
Breaking Biology Technology:First Confirmed Detection of a Complete Molar Pregnancy with Cell-Free DNA Test 2First Confirmed Detection of a Complete Molar Pregnancy with Cell-Free DNA Test 3RXi Pharmaceuticals Announces $10 Million Public Offering of Common Stock and Warrants 2RXi Pharmaceuticals Announces $10 Million Public Offering of Common Stock and Warrants 3Danielle Vitiello, MD of Fertility Centers of New England, Named New Medical Director 2American Process Inc. Announces Partnership with Oak Ridge National Laboratory to Make 3D Printing Plastic Resins Stronger and More Sustainable 2American Process Inc. Announces Partnership with Oak Ridge National Laboratory to Make 3D Printing Plastic Resins Stronger and More Sustainable 3
... system receives Health Canada clearance to treat severe ... vascular dyschromia and wrinkles, MOUNTAIN VIEW, Calif., Aug. ... and market leader of aesthetic,laser skin treatments, today announced ... the recent Health Canada Clearance. The Canadian,debut of the ...
... Pork Back to Farm of Origin, NORTHFIELD, ... Brand, a leader in premium, all-natural fresh pork, ... DNA for reliable and accurate,traceability back to the ... use IdentiGEN,s DNA TraceBack(R) system, a USDA Process,Verified, ...
... ReceptorBio,Inc. announced today that it has entered into ... (Nasdaq: TELK ) that would enable ReceptorBio ... insulin,receptor activators. These compounds were identified through the,application ... provides for payments related to regulatory milestones and,royalties ...
Cached Biology Technology:Reliant Technologies Announces Canadian Launch of Fraxel re:pair(R) Laser: A New Class of Treatment Therapy for CO2 Laser Skin Resurfacing 2Reliant Technologies Announces Canadian Launch of Fraxel re:pair(R) Laser: A New Class of Treatment Therapy for CO2 Laser Skin Resurfacing 3Reliant Technologies Announces Canadian Launch of Fraxel re:pair(R) Laser: A New Class of Treatment Therapy for CO2 Laser Skin Resurfacing 4Nature's Premium(R) Brand First Fresh Pork in North America to Carry DNA TraceBack(R) Seal of Authenticity 2Nature's Premium(R) Brand First Fresh Pork in North America to Carry DNA TraceBack(R) Seal of Authenticity 3Nature's Premium(R) Brand First Fresh Pork in North America to Carry DNA TraceBack(R) Seal of Authenticity 4ReceptorBio Announces Diabetes License Agreement With Telik 2ReceptorBio Announces Diabetes License Agreement With Telik 3
(Date:5/14/2015)... Research and Markets ( http://www.researchandmarkets.com/research/5whpxg/fingerprint ) ... Market in Smart Mobile Devices " report to ... was a watershed year for fingerprint sensors in smartphones ... Apple gave fingerprint sensors a raison d,etre in the ... are a must-have feature in flagship smartphones. ...
(Date:5/11/2015)... 11, 2015  Synaptics Incorporated (NASDAQ: SYNA ... today announced the appointment of Wajid Ali ... Officer, reporting to Rick Bergman , President ... Financial Officer, Kathleen Bayless , who announced ... Mr. Ali brings extensive financial management expertise to ...
(Date:5/11/2015)... N.Y. , May 11, 2015 Curemark ... announced the start of a new Phase III double ... of its novel formulation, CM-AT, on all children ages ... of its FDA Phase III double blinded clinical trial ... had low levels of the digestive enzyme chymotrypsin. This ...
Breaking Biology News(10 mins):Global Smart Mobile Devices Fingerprint Sensors Market 2015-2019: Key Vendors are Authentec, Validity Sensors, Fingerprint Cards, Next Biometrics Group. IDEX, and Qualcomm 2Synaptics Appoints Wajid Ali as Senior Vice President and Chief Financial Officer 2Curemark, LLC, Launches New Phase III Trial in Expanded Population of Children with Autism 2
... the Octopusthe eight-legged oracle who made international headlines with ... in the sea. The Indonesian mimic octopus, which can ... may well give Paul a run for his money ... configuring its limbs, adopting characteristic undulating movements, and displaying ...
... Stanford mechanical engineer is using the biology of a gecko,s ... same way the small reptile can scale a wall of ... modeled on the intricate design of gecko toes. , Mark ... mechanical engineering and co-director of the Center for Design Research, ...
... urgently needed in Northern Ireland to ensure the continued ... at Queen,s University Belfast. Lizards are found in ... a Queen,s study, published in international journal Amphibia-Reptilia ... replaced through agricultural intensification. "The fact that Northern ...
Cached Biology News:Octopus mimics flatfish and flaunts it 2Octopus mimics flatfish and flaunts it 3Secrets of the gecko foot help robot climb 2Secrets of the gecko foot help robot climb 3No lounge for local lizards as living room vanishes 2
Sprague-Dawley rats, 7-8 weeks old, mixed gender; tissue is collected fresh, frozen on dry ice within minutes of sacrifice and stored at -20C; individual tissue weight approx. 0.05 gram....
...
Contains L-glutamine...
EMBP (FL-222)...
Biology Products: