Navigation Links
Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000

Lynn Jordan, James Batchelor, and Ed Alderman
Applied Science and Technology Department, Caliper Life Sciences Inc., Hopkinton, MA 01748

Kelly M. Clark, Joseph A. Hensley and Robert P. Weisbein
Brinkmann An Eppendorf Company, Westbury, NY

Jennifer L. Halcome and George R. Halley
Eppendorf 5 Prime, Inc. Boulder, CO

Introduction

To assemble large genome constructs, Bacterial Artificial Chromosomes (BACs) are necessary for fingerprinting mapping and BAC-end sequencing. It is critical to have an automated method to obtain high quality BAC DNA. We have completed feasibility studies integrating Eppendorfs Perfectprep BAC 96 reagent kit with Caliper Life Sciences Sciclone ALH 3000 to achieve high quality BAC in a timely fashion. A 96- channel pipetting head performs reagent additions using disposable tips. An on-deck shaker is used to resuspend the pelleted material, and is used for mixing in other steps. Positive pressure is used to perform all filtrations steps. Through the use of an on-deck gripper, plates can be relocated on the deck to provide a Hands-Off automated solution.

Introduction

Purification of sufficient quantities of high quality BAC DNA, when using lower volumes of reagent and starting culture, has posed a challenge for researchers. Eppendorf has solved this problem with the development of the Perfectprep BAC 96 that is capable of processing 96 samples in a microplate format. Figure 1 shows an overview of the Prefectprep BAC 96 process. For many researchers, projects involve the processing of thousands of samples to acquire the downstream data required. In an effort to achieve low- to mid- throughput purification (1-16 plates in an eight-hour workday), we have performed initial experiments to integrate the Eppendorf Perfectprep BAC 96 kit onto the Caliper Life Sciences Sci
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. RNAqueous-96 RNA Isolation Kit Automation Protocol
2. Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor
3. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
4. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
5. Eppendorf UVette Material Compatibility
6. Eppendorf Perfectprep Gel Cleanup Kit
7. Eppendorf Perfectprep Gel Cleanup Kit
8. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
9. Eppendorf Electroporation Application Notes Your participation is highly welcome !!!
10. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
11. EppendorfPerfect gDNA Blood Mini Kit
Post Your Comments:
(Date:9/18/2014)... YARDLEY , Pennsylvanie, 18 septembre 2014 ... Lyon en 1974, a été ... à compléter les évaluations cliniques traditionnelles par ... Reported Outcomes) et sur les impacts économiques ... http://photos.prnewswire.com/prnh/20140618/119365 A cours ...
(Date:9/18/2014)... Now a team led by an engineer at Michigan ... that will let scientists slash the cost of one ... Syringe pumps are used to dispatch precise amounts of ... a reaction. They can also cost hundreds or even ... of Michigan Tech students published the library of free ...
(Date:9/18/2014)... (PRWEB) September 18, 2014 Silicon-valley Electronic ... with Digitaliza TXT , the latest addition to ... its Clinical Research Organization (CRO) Program, Clinovo further expands ... three days of ClinCapture training, we are now able ... CEO Ana Pulido. “We can also rely on Clinovo ...
(Date:9/17/2014)... 2014 A long-term retrospective study of ... (APBI) via interstitial brachytherapy suggests that women younger ... could be suitable to receive the treatment. Current ... these patients in the "unsuitable" category for APBI. ... meeting, Beaumont Health System,s Peter Y. Chen ...
Breaking Biology Technology:1974 - 2014 - Mapi fête ses 40 ans 2Doing science just got cheaper -- and faster 2Digitaliza TXT Joins Clinovo’s CRO Partnership Program, Bringing Its Open Source EDC System, ClinCapture to Latin America 2Digitaliza TXT Joins Clinovo’s CRO Partnership Program, Bringing Its Open Source EDC System, ClinCapture to Latin America 3Partial Breast Irradiation With Interstitial Brachytherapy Appears Appropriate For Women Previously Considered Unsuitable 2Partial Breast Irradiation With Interstitial Brachytherapy Appears Appropriate For Women Previously Considered Unsuitable 3Partial Breast Irradiation With Interstitial Brachytherapy Appears Appropriate For Women Previously Considered Unsuitable 4
... my wishes are,coming true," says Kristin Shumpert, who is traveling from ... of Las,Vegas ... and to meet the people who "saved my ... held little interest to the young,woman who suffers from a devastating ... time to use the ladies, room. , ...
... Integrated Knowledge Center Outside the United States. , ... ... Knowledge Center (DKC) today, 17 months after breaking ground last summer. ... the city’s Genome Valley, the center will be a research hub ...
... commercial life sciences sales experience to Cyntellect , ... a privately-held life sciences company leading the development and commercialization ... it has appointed Andre Lubarsky as the Company,s Director of ... sales of Cyntellect,s cell purification and cell health products in ...
Cached Biology Technology:Noninvasive Medical Technologies Helps Make Young Tennessee Woman's Wishes Come True 2Noninvasive Medical Technologies Helps Make Young Tennessee Woman's Wishes Come True 3Noninvasive Medical Technologies Helps Make Young Tennessee Woman's Wishes Come True 4Noninvasive Medical Technologies Helps Make Young Tennessee Woman's Wishes Come True 5DuPont Opens Research & Development Center in India 2DuPont Opens Research & Development Center in India 3Cyntellect Appoints Former BD Biosciences Executive Andre Lubarsky as Director of Sales - North America 2
(Date:9/18/2014)... AZ, Sept. 18, 2014 -- Arizona turned its newborn ... just a few short months, earning it the first-ever Newborn ... The March of Dimes presented Will Humble, M.P.H., Arizona,s Department ... held here today. Mr. Humble established a policy of full ... to send newborn blood samples to the lab for analysis, ...
(Date:9/18/2014)... will be celebrated tonight at the third annual Golden ... health of premature infants and in paving the way ... work was supported by the National Science Foundation, the ... Commission, will be honored at a ceremony at the ... of Members of Congress will be on hand to ...
(Date:9/18/2014)... September 18, 2014 Elsevier, a world-leading provider ... services, and the Australasian Research Management Society (ARMS), ... scientists at the 5th Scopus Young Researcher of ... earlier today at the National Convention Centre and ... was attended by guests representing Australia,s and New ...
Breaking Biology News(10 mins):March of Dimes honors Arizona with first-ever newborn screening award 2March of Dimes honors Arizona with first-ever newborn screening award 33rd annual Golden Goose award ceremony honors 8 researchers; Unusual work had big results 2Elsevier and Australasian Research Management Society award scientists at Scopus ceremony 2
... fluctuations in the populations they target, but its not ... journal Nature by scientists at Scripps Institution of Oceanography ... general underlying mechanism. Research led at Scripps with ... two chief scientific advisors to the United Kingdom) demonstrates ...
... worm, University of Maryland researchers have discovered an important ... absorbed and transported into the body. The finding could ... the worlds number one nutritional disorder., With C. elegans, ... Hamza, assistant professor of animal and avian sciences, and ...
... -- Some primates have evolved big brains because their ... advantage that outweighs the demands of extra years of ... Duke University and the University of Zurich have concluded ... key benchmarks in the development of 28 different primate ...
Cached Biology News:Fishing throws targeted species off balance, Scripps study shows 2Fishing throws targeted species off balance, Scripps study shows 3Bloodless worm sheds light on human blood, iron deficiency 2Bloodless worm sheds light on human blood, iron deficiency 3Slowly-developing primates definitely not dim-witted 2Slowly-developing primates definitely not dim-witted 3
Mouse monoclonal antibody to Lats2....
... (eIF4E-binding protein II) is involved in translation ... kidney. PHAS proteins bind to eIF-4E, the ... capped mRNA in vitro and in cells. ... of PHAS/eIF-4E complexes. This allows eIF-4E to ...
MAb to HSV-2, gG-2 HSV-2 Glycoprotein G-2...
... INK4a-ARF locus encodes two unrelated proteins both ... arrests the cell cycle in a p53-dependent ... the rapid degradation of mdm2 proteinrequired for ... accumulation. This interaction is mediated by ...
Biology Products: