Navigation Links
Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor

m quantities of DNA are amplified in all samples, eliminating the need for quantitation.

GenomiPhi DNA Amplification Kit representatively amplifies single- or double-stranded linear genomic DNA using Phi29 DNA polymerase to perform strand displacement amplification. Microgram quantities of representative DNA are produced from small amounts of starting material that can be used immediately in downstream applications.

For all kits, DNA amplification is achieved by combining the starting DNA with random hexamer primers, Phi29 DNA polymerase, dNTPs, and buffer components optimized for DNA synthesis. This reaction mixture is incubated overnight at 30 °C, during which time the available nucleotides are consumed and converted into high molecular weight fragment copies of the input DNA.


Automation
The amplification protocols are simple and allow straightforward automation. The Genesis Freedom series of RSPs from Tecan are ideal for automating DNA amplification reactions and downstream applications. The arm of the Tecan liquid handler is equipped with four or eight tips and can perform all required liquid handling steps with high sensitivity and accuracy. When the amplification reaction stops because the nucleotides in the reaction are exhausted, DNA yields are equivalent across all samples (Fig 2). Disposable tips eliminate cross-contamination concerns. With an eight-channel liquid handling arm, multiple 96-well plates of TempliPhi or GenomiPhi DNA amplification reactions can be prepared in half the time of manual pipetting. Whatever the post-amplification application (Fig 3), the open platform can be adapted. The Tecan Gemini software system allows easy protocol setup and operation of the workstation.



Conclusions
The combination of GE Healthcare’s DNA amplification products and
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. RNAqueous-96 RNA Isolation Kit Automation Protocol
2. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
3. Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000
4. Oragene and Whole Genome Amplification with GenomiPhi
5. GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH
6. GenomiPhi amplification of FFPE tissue DNA for array CGH
7. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
8. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
9. Greater Amplification Specificity with New Hot Start PCR Enzyme
10. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
11. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
Post Your Comments:
(Date:1/15/2014)... 2014 2013 was a banner ... Brain State Technologies®. They saw continued independent research ... Medical Center who were awarded a $1 million ... research papers published in “Brain and Behavior” a ... in First for Women Magazine and they were ...
(Date:1/14/2014)... 2014 Carahsoft and CDS Federal ... January 23, 2014 at 2pm EST (11am PST), ... Knowledge.” The topic focuses on how technology can ... drive mission critical decisions for government agencies. The ... Synopsis: Big Data has become an obstacle for ...
(Date:1/14/2014)... Texas , Jan. 14, 2014 ... analyze and research medicinal plants and therapeutic derivatives ... industry, health professionals, and researchers about the challenges ... (Logo: http://photos.prnewswire.com/prnh/20100430/DC95601LOGO ) The ... (known by its acronym GA, based on ...
(Date:1/14/2014)... 2014 Global Record Systems, LLC, ... technology solutions for patients, physicians, the biopharmaceutical industry, ... today the signing of a three-year Research Collaboration ... Administration (FDA). This initiative is designed to ... safety, clinical outcomes, and medication adherence. , It ...
Breaking Biology Technology:Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8Global Record Systems Announces Research Collaboration Agreement with FDA to Create a Novel “Big Data” Paradigm for Collection of Patient Safety and Outcomes Information 2
(Date:4/15/2014)... researchers have previously shown that a genetic pre-disposition to ... a new study, Frank Booth, a professor in the ... link between the genetic pre-disposition for high levels of ... occurs. , For his study, Booth selectively bred rats ... laziness. Booth then put the rats in cages with ...
(Date:4/15/2014)... Western Reserve University dental researchers have found a ... cells from the mouth to study how the ... , By isolating some specialized immune cells (white ... they fight diseases in the mouthor reject foreign ... to learn more about treating and preventing such ...
(Date:4/15/2014)... There are many viral diseases in the world for ... dengue fever, which is spread by mosquitoes in the ... is more familiar in Finland and is easily spread ... small children and the elderly. , Researchers at Aalto ... succeeded in preliminary tests to prevent the spread of ...
Breaking Biology News(10 mins):Genetic pre-disposition toward exercise and mental development may be linked 2New method isolates immune cells for researchers to study how they ward off oral diseases 2Nanocrystalline cellulose modified into an efficient viral inhibitor 2
... University of California, San Diego School of Medicine say that ... molecule boosted disease protection in early hominids, and may have ... Homo . The findings, published in this week,s early ... of Sciences , are among the first evidence of a ...
... Research by biologists at the University of York and ... about the way the brain is affected by age. ... Medicine and Dentistry in Plymouth, they have studied responses ... The researchers discovered that under stressful conditions, such as ...
... off to Antarctica for the first stage of an ambitious ... lake buried beneath three kilometres of solid ice. This extraordinary ... knowledge about the evolution of life on Earth and other ... climate. Transporting nearly 70 tonnes of equipment the ,advance ...
Cached Biology News:Sexual selection by sugar molecule helped determine human origins 2Sexual selection by sugar molecule helped determine human origins 3Researchers study aging's effect on the brain 2Engineering team heads to Antarctica to explore hidden lake 2Engineering team heads to Antarctica to explore hidden lake 3
COMING SOON: Anti-Mouse C5/C5b, Clone BB5.1. Monoclonal Antibody...
... Goat anti-Human Alpha 1 Acid ... azide Goats were immunized ... glycoprotein in Freund's adjuvant. ... absorbed to ensure specificity. ...
Anti-Mouse Valpha 2 TCR, Purified (Clone B20.1) (rat IgG2a)...
Request Info...
Biology Products: