Navigation Links
Automating RNA Isolation

esults from the analysis of 9 samples from K562 cells are shown in Figure 2. Cycle threshold (Ct) values, which reflect the amount of target RNA present in the sample, were calculated (Figure 3). Although some variation in yield between replicate wells was observed, it is most likely due to several factors unrelated to the isolation method, including variation in the number of cells manually dispensed to each well, and variation in cell growth and turnover during incubation. Importantly, the consistent ratio of hTERT to GAPDH in replicate samples indicates that both high abundance and low abundance mRNAs are reproducibly isolated with the automated RNAqueous-96 protocol.

Figure 2.Real-time RT-PCR Detection of GAPDH and hTERT in Replicate Samples Isolated Using the RNAqueous-96 Automation Protocol. RNA was isolated as described in Figure 1. Samples were analyzed by real-time RT-PCR using primers and a TaqMan probe for GAPDH (A.) or hTERT (B.) in an ABI 7700 Sequence Detector. Data from 9 samples, each indicated by a different color line, are shown here. The average cycle threshold (Ct) values for all of the samples are shown in Figure 3.


'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Microorganisms
7. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
8. Mouse Tail Genomic DNA Isolation Protocol(1)
9. Genomic DNA Isolation Protocol(1,2,3)
10. Basic Plasmid DNA Isolation Protocol
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
Cell Line Target Average Ct Value Standard Deviation
K562 GAPDH 15.86 0.45
hTERT
*Name:
*Comment:
*Email:
TAG: Automating RNA Isolation

(Date:11/27/2014)... , Nov. 27, 2014 ... the "Global Palmitic Acid Industry Report 2014" ... The Global Palmitic Acid Industry ... the current state of the global palmitic acid industry. ... the industry including definitions, classifications, applications and industry chain ...
(Date:11/27/2014)... England , November 27, 2014 ... online on ScienceDirect   Elsevier , ... products and solutions, is pleased to announce the launch ... . The launch of ... the rapid advances at the forefront of applied sciences ...
(Date:11/27/2014)... BELLINGHAM, Washington, and PITTSFIELD, Massachusetts, USA (PRWEB) November 26, ... pinpoint cancer cells with more accuracy, additive systems to ... improve highway safety by analyzing road conditions are among ... Awards for Photonics Innovation . The awards are sponsored ... photonics , and Photonics Media . , Winners ...
(Date:11/26/2014)... 2014 The report on the Global ... the concerned market for revenue forecasts and market trends. ... of 51.9% from 2013 to 2018. At the projected ... of $1,415.6 million by 2018. , Browse through the ... for information about the market segmentation and in-depth analysis ...
Breaking Biology Technology:Global Palmitic Acid Industry Report 2014 2Elsevier Announces the Launch of a New Journal: Extreme Mechanics Letters 2Elsevier Announces the Launch of a New Journal: Extreme Mechanics Letters 3Finalists Named for 2015 Prism Awards for Photonics Innovation 2Finalists Named for 2015 Prism Awards for Photonics Innovation 3Finalists Named for 2015 Prism Awards for Photonics Innovation 4Finalists Named for 2015 Prism Awards for Photonics Innovation 5Global Smart Sensor Market in Building Automation is expected to reach a value of $1,415.6 million by 2018 - Report by MicroMarket Monitor 2Global Smart Sensor Market in Building Automation is expected to reach a value of $1,415.6 million by 2018 - Report by MicroMarket Monitor 3Global Smart Sensor Market in Building Automation is expected to reach a value of $1,415.6 million by 2018 - Report by MicroMarket Monitor 4
... ... ... -- Artemis Health Institute , a leading international hospital located just outside Delhi, is ... hospital recently reported a 10% month on month increase in medical tourist admissions, and now ...
... invented by a Monash University researcher could dramatically ... Monash biomedical engineer Brian Lithgow has developed ... for the mind,. Patterns of electrical activity in ... against distinct response patterns found in depression, schizophrenia ...
... A team of scientists, led by a biomedical engineer at ... the first time that mathematical models created from data ... previously unknown cellular mechanisms. This brings biologists a step closer ... inner workings of the cell as readily as NASA engineers ...
Cached Biology Technology:Artemis Continues to Attract More Medical Tourists from USA 2Artemis Continues to Attract More Medical Tourists from USA 3'ECG for the mind' could diagnose depression in an hour 2Scientists use math modeling to predict unknown biological mechanism of regulation 2
(Date:11/4/2014)... the right amount of death at the right time ... to new research that could help in understanding animal ... , In a paper in the journal Trends ... and European colleagues conclude that the kind of positive ... of individuals, or mortality, depends on the size and ...
(Date:11/4/2014)... about the way our bodies are assembled during early ... they are supposed to become a nerve or a ... correct place and alignment? Researchers at the University of ... a new study, UM researchers describe the signaling systems ... at the head-trunk region. Their discovery may have important ...
(Date:11/4/2014)...   3D scanner ... Fuel3D , a developer of 3D scanning solutions, ... $6.4 million (£4 million). This funding builds on the company,s ... and paves the way for the commercial launch of the ... round was led by Chimera Partners and will be used ...
Breaking Biology News(10 mins):When less is more: Death in moderation boosts population density in nature 2The inside story: How the brain and skull stay together 2Fuel3D Secures $6.4 Million in Expansion Funding 2Fuel3D Secures $6.4 Million in Expansion Funding 3
... into a tumor, seal the exits and detonate a ... cells unscathed. , MIT researchers have designed a nanoparticle ... "nanocell" proved effective and safe, with prolonged survival, against ... mice. , The work will be reported in the ...
... Science Express http://www.sciencexpress.org) on July 28th reveals a striking ... open ocean ?the largest and least known part of ... and fishing over the past 50 years, the authors ... surprising global pattern of open ocean hotspots - areas ...
... how organisms grow and flourish, but studying their complex ... Now, a team of chemists and neurobiologists led by ... M. Schuman at the California Institute of Technology has ... synthesis in specific locations. , The new method, which ...
Cached Biology News:MIT engineers an anti-cancer smart bomb 2Scientists discover global pattern of big fish diversity in open oceans 2Scientists discover global pattern of big fish diversity in open oceans 3Scientists discover global pattern of big fish diversity in open oceans 4Scientists discover global pattern of big fish diversity in open oceans 5Protein synthesis can be controlled by light, opening way for new scientific, medical applications 2Protein synthesis can be controlled by light, opening way for new scientific, medical applications 3
Recombinant Human Erythropoietin (Tissue Culture Grade)...
One-step, microplate or cuvet, colorimetric, detection range 0.78 ug/dL to 65 ug/dL Zn in serum, plasma, urine, saliva etc. Procedure: 10 min....
Human PDGF R beta MAb (Clone # PR7212)...
Recombinant Mouse IL-23, CF...
Biology Products: