Navigation Links
Automating RNA Isolation


Large scale gene expression screening projects require reliable high-throughput methods to isolate high quality RNA from a large number of samples. To meet this growing need, Ambion developed the RNAqueous-96 Kit. This kit, which utilizes an RNA-binding glass-fiber filter, provides high yields of intact RNA in a 96-well plate format. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. Because the kit is compatible with commonly used 96-well plate vacuum manifolds, the procedure is readily automated for use with high throughput liquid handling systems.

Recently, Ambion, in collaboration with PerkinElmer Life Sciences, developed an automation protocol for the RNAqueous-96 Kit using the Packard MultiPROBE II HT Liquid Handling System. This method reproducibly yields high quality total RNA from both adherent and suspended mammalian cultured cells.


Reproducible Isolation of High Quality RNA
To demonstrate the high quality of RNA that can be obtained using the RNAqueous-96 Kit on a robotic platform, RNA from multiple HeLa S3 and K562 cell samples was isolated using the method described in "RNAqueous-96 Automation Protocol". Briefly, HeLa S3 (adherent) and K562 (suspended) cells were transferred to a deep well 96-well plate at 3.75 x 105 cells/well. HeLa cells were incubated for 2 days prior to RNA isolation, whereas K562 cells were processed immediately. The resulting RNA was assessed for both yield and qua
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Microorganisms
7. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
8. Mouse Tail Genomic DNA Isolation Protocol(1)
9. Genomic DNA Isolation Protocol(1,2,3)
10. Basic Plasmid DNA Isolation Protocol
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Automating RNA Isolation

(Date:7/3/2015)... 2015 Forskare kan ... på  http://www.openinnovationinscience.at till det återkommande utbildningsprogrammet ... hålls i Wien .  ... internationella forskare och vetenskapsmän som genomfördes av ... största utmaningar bristen på incitament för att ...
(Date:7/2/2015)... ... ... in countless labs for decades. In the past, people had to go to a ... a daunting and tiring process looking at endless data tables and charts. Often time’s, ... catalog structure. , Now thanks to FireflySci, scientists are throwing away those old books and ...
(Date:7/2/2015)... Calif. (PRWEB) , ... July 02, 2015 , ... Sleepless ... improve an individual’s quality of sleep, launched its Indiegogo campaign on June 23. Sound ... Luzi's voice command technology makes it effortless to use, while the integration of the ...
(Date:7/1/2015)... 1, 2015   Decision Resources Group ... of Otezla are its oral administration, alternative mechanism ... by surveyed rheumatologists. Otezla was the first oral ... of active psoriatic arthritis in the ... care includes conventional oral treatments including disease-modifying antirheumatic ...
Breaking Biology Technology:Lansering av världens första program för öppen innovation för att utbilda forskare 2Lansering av världens första program för öppen innovation för att utbilda forskare 3New Cuvette E-Commerce Website Launched by FireflySci 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 3Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 2Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 3
... plug-in for popular embedded tool suite. , ... ... Systems, Inc., a leading supplier of configurable and scalable real-time ... for the RTXC Quadros RTOS is now available for the ...
... in 16 Specialties, ... Hospitals Included in "Honor Roll", WASHINGTON, July 11 ... Best Hospitals, accessible,today online at http://www.usnews.com/besthospitals and on ... 2008 America,s Best Hospitals guide,ranks 170 medical centers nationwide in ...
... study using the CiTop™ technology in the coronary arteries. Ovalum ... today successful experiences using CiTop™ ExPander™ Guidewire in traversing chronic ... ... Rehovot , Israel (PRWEB) July 11, 2008 -- ...
Cached Biology Technology:RTXC Kernel Awareness Module from Quadros Systems Now Available for Keil Vision Debugger 2RTXC Kernel Awareness Module from Quadros Systems Now Available for Keil Vision Debugger 3RTXC Kernel Awareness Module from Quadros Systems Now Available for Keil Vision Debugger 4U.S. News Media Group Releases 19th Edition of America's Best Hospitals 2U.S. News Media Group Releases 19th Edition of America's Best Hospitals 3Ovalum Ltd. Announces Success in Coronary Cases Using CiTop™ ExPander™ Guidewire 2
(Date:6/24/2015)... June 24, 2015  Synaptics Inc. (NASDAQ: ... solutions, today announced that Sharp has selected its ... authentication for its latest flagship smartphone device, the ... 200 million shipments of its fingerprint ID sensor ... strength, scalability and leadership in the biometrics market ...
(Date:6/23/2015)... RALEIGH, N.C. , June 23, 2015 /PRNewswire/ ... technology, today announced the results of a recent ... unmatched accuracy on the wrist during activity. In ... biometric technology was compared along with the Apple ... for accurately measuring heart rate during activity. The ...
(Date:6/17/2015)... , and HILDEN, Germany , June ... QGEN ; Frankfurt Prime Standard: QIA) today launched new Investigator ... in forensic laboratories in the United States ... to simultaneously analyze multiple key genomic markers (short tandem repeats ... Quality Sensor to evaluate the quality of DNA in ...
Breaking Biology News(10 mins):Synaptics Brings Natural ID Technology to Sharp's Newest Flagship Smartphone 2Valencell Delivers Breakthrough Heart Rate Accuracy on the Wrist 2Valencell Delivers Breakthrough Heart Rate Accuracy on the Wrist 3QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 2QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 3QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 4QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 5
... stand out and attract mates, according to a new study ... Seville, Spain, and colleagues. Their research is the first to ... secretions of their uropygial gland for cosmetic reasons. The uropygial ... and is situated near the base of the tail. The ...
... may have a negative effect on Sweden,s golden eagles. ... the Swedish University of Agricultural Sciences (SLU) are trapping ... The satellite transmitters emit a signal once an ... with a picture of how the birds use the ...
... Science Applications International Corporation (SAIC) (NYSE: SAI ... contract by the U. S. Department of Defense (DoD), Biometrics ... to the U.S. Central Command,s (USCENTCOM) area of ... period of performance, two one year options and a total ...
Cached Biology News:Beauty from the bottom up 2Golden eagles studied by satellite 2SAIC Awarded $23 Million Task Order by Defense Biometrics Identity Management Agency 2
Anti-Mouse C-10, Biotin (Polyclonal) (rabbit IgG)...
Anti-rat Dipeptidyl Peptidase IV (DPP IV), CD26, Clone 5E8, Monoclonal Antibody...
... H3 (Lys 4) Immunogen: ... ...RT[ Ac K]Q... in which [ Ac ... human histone H3 Accession ... Weight: Mr ~17kDa ...
Anti-Mouse Ly 6C.2 , Ascites (Clone 5075-3.6) (mouse IgG2b)...
Biology Products: