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Automated RNA Amplification for Arrays

hybridization results: reproducible gene-expression patterns, low background, and high signal-to-noise ratios.

Figure 2. Consistent, Reproducible Hybridization.HL60 aRNA (1 g/well; prepared with the automated methods described earlier) was hybridized in 96 wells of GeneXP Bioscience's BGA plate containing identical arrays of 50mer oligonucleotides designed to human target genes. Hybridizations were performed overnight in 80 l hybridization buffer using fragmented amino Allyl Cy5 aRNA (1 g) prepared from HL60 total RNA. Plates were processed using a Biomek FX (Beckman Coulter). Arrays were imaged using the Tecan LS200 scanner. The inset shows the hybridization results for four adjacent wells of the 96 BioGrid Array plate.


GeneXP has had excellent results incorporating Ambion's Amino Allyl MessageAmp aRNA Amplification Kit into its automated, high-throughput gene expression BGA platform. Expert tailoring of robot motion to the unique requirements of the kit and cleanup with magnetic particles were instrumental in developing a reproducible high-throughput process. Automation of the Amino Allyl MessageAmp aRNA Amplification Kit has enabled GeneXP to offer customers fast, affordable, and reliable high-throughput gene expression studies.

For more information visit http://info.genexpbiosciences.com/,
email info@genexpbiosciences.com, or call (781) 937-0505.


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