Navigation Links
Automated RNA Amplification for Arrays


Karen Rose, Sonal Patel, and David Quilter (GeneXP Biosciences Inc.)
  • Efficient and cost-effective method for synthesizing aRNA in a high throughput format
  • Increase throughput by >10-fold
  • Rapid, reliable, and consistent results

Life science researchers need an efficient and cost-effective technique to profile gene expression in multiple biological samples. Ambion's collaboration with GeneXP Biosciences, Inc. has resulted in the integration of a high throughput version of Ambion's Amino Allyl MessageAmp aRNA Amplification protocol with GeneXP's BioGridArray (BGA), producing a valuable tool that enables scientists to conduct hundreds or thousands of focused gene expression experiments at a fraction of the time and cost of other methods. The BGA uses arrays containing up to 1,024 features in each well of a standard microplate enabling 96-fold improvement in throughput. Hybridization setup and post-hybridization washes are performed with a robotic liquid handler, which reduces variability, processing time, and labor. Using the MessageAmp aRNA Amplification protocol, GeneXP's BGA typically delivers coefficients of variance below 15% with sensitivity to 1 mRNA spike molecule in 250,000 human transcripts.

A significant challenge to large scale expression analysis and profiling projects is sample preparation. For example, manual sample preparation using Ambion's Amino Allyl MessageAmp aRNA Amplification Kit is typically limited to 1020 aRNA preparations over a two-day period, or approximately 2040 samples per week. The rate limiting step for increasing sample q
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Purified Single-Stranded M13 DNA for Automated Sequencing
2. Automated Fluorescent-Tag Cycle Sequencing
3. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
4. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
5. Agilent 2100 Bioanalyzer Automated Analysis System
6. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
7. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
8. Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents
9. DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
10. Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry
11. Automated Dissolution of Prednisone with the MultiDose G3
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Automated RNA Amplification for Arrays

(Date:1/22/2015)... Cambridge Semantics, the leading provider of smart data solutions driven ... record-breaking year across the board for the company. , ... and our Smart Data solutions, our customers were able to ... to record growth for the company in 2014,” said Alok ...
(Date:1/22/2015)... Protocol Networks brings independent technology consulting to ... independent consultants, Protocol Networks has recently announced its expansion ... years, his company has attracted several clients in The ... well as others. With the success of these engagements, ...
(Date:1/22/2015)... Pipette.com has added the Eppendorf Centrifuge ... of Eppendorf products. , The Eppendorf Centrifuge 5424/5424 R ... R and receive the following:, ,     Free ... Eppendorf Reference 2 ,     3 Free boxes ...
(Date:1/22/2015)... Dr. Greg Leyer of UAS Labs recently ... Supplements Pre-Conference seminar on probiotics in San Diego, CA. ... education conference for health care professionals. This year’s pre-conference seminar ... probiotics in health. Dr. Leyer spoke about the emerging topics ...
Breaking Biology Technology:Cambridge Semantics Announces Record Results for 2014 2Protocol Networks Brings Independent Technology Consulting to the Constitution State 2Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2
... multi-drug ... resistant infections -, NEW HAVEN, Conn., Jan. ... focused on the,development of novel antibiotics for the treatment of antibiotic-resistant,infections, ... 2 clinical trials. The studies will evaluate the safety and,efficacy of ...
... the pharmaceutical and biotechnology ... strengthens specialist expertise, BOSTON, Jan. 3 Observant ... a passion for research excellence,and a commitment to client ... joined the firm as Vice President. Dr. Hartman,s,research has ...
... are using the tiniest organisms on the planet ,bacteria, as ... study featured in the journal Biotechnology and Bioengineering, lead author ... have gained critical insights that may lead to commercialization of ... can use any kind of waste, such as sewage or ...
Cached Biology Technology:Rib-X Pharmaceuticals Initiates Two Phase 2 Studies for Novel Antibiotic Compound RX-1741 2Rib-X Pharmaceuticals Initiates Two Phase 2 Studies for Novel Antibiotic Compound RX-1741 3Observant LLC Expands Senior Management Team 2Life at the jolt 2Life at the jolt 3Life at the jolt 4
(Date:12/17/2014)... N.C. , Dec. 16, 2014 Valencell, a ... its PerformTek biometric technology to industry leaders such as ... accurate, clinically validated, biometric wearable products. These products will ... in Las Vegas . ...
(Date:12/11/2014)... Dec. 09, 2014 Research and Markets ... http://www.researchandmarkets.com/research/twhjll/biometrics_market ) has announced the addition of the ... their offering. One major trend emerging ... advances in technology, it is important to upgrade biometric ...
(Date:12/10/2014)... You,ve been here before: you desperately need to sign into your ... the answer to your secret question. What,s your dog,s birthday? Who ... Labs , a digital infrastructure security company, launches the ... comes with usernames, passwords and PINs – 1U TM ...
Breaking Biology News(10 mins):Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3
... the most rapidly increasing food production system worldwide through ... 2009 issue of BioScience . The assessment, by ... Ann Arbor, notes that despite well-publicized concerns about ... practiced well, be no more damaging to biodiversity than ...
... has worked out how some newly discovered light sensors ... brain. The report appears online this week in ... small number of nerve cells in the retina that ... retinarods and conesmelanopsin-containing cells are not used for seeing ...
... DNA modification in lung cancer, a team of international researchers ... They say that "silencing" of a single gene in lung ... cells, contributing to cancer development and progression. In the ... journal of the American Association for Cancer Research, they also ...
Cached Biology News:Aquaculture's growth seen as continuing 2Bright lights, not-so-big pupils 2Bright lights, not-so-big pupils 3In lung cancer, silencing one crucial gene disrupts normal functioning of genome 2In lung cancer, silencing one crucial gene disrupts normal functioning of genome 3
... Two separate vials containing 200 ... same sequence used as antigens for ... 160890) To be ... cyclase polyclonal antiserum (Catalog No. 160890) ...
... Clone/PAD: 3B8A10. Immunogen: ... the C-terminal region of ... Specific for the human ... Human (positive control: 293 ...
... The PinPoint™ Vector Sequencing Primer is ... PinPoint™ Xa Vectors (Cat.# V2031, V2051, V2061). ... Xa site at nucleotides 325343, approximately 4050 ... region and can be used to determine ...
... The versatile Zymo-Spin I-96 Plate can be ... manifolds for the large-scale (i.e., 96-well) purification ... polypropylene construction and unique silica-based matrix make ... g DNA or RNA in = 10 ...
Biology Products: