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Automated RNA Amplification for Arrays


Karen Rose, Sonal Patel, and David Quilter (GeneXP Biosciences Inc.)
  • Efficient and cost-effective method for synthesizing aRNA in a high throughput format
  • Increase throughput by >10-fold
  • Rapid, reliable, and consistent results

Life science researchers need an efficient and cost-effective technique to profile gene expression in multiple biological samples. Ambion's collaboration with GeneXP Biosciences, Inc. has resulted in the integration of a high throughput version of Ambion's Amino Allyl MessageAmp aRNA Amplification protocol with GeneXP's BioGridArray (BGA), producing a valuable tool that enables scientists to conduct hundreds or thousands of focused gene expression experiments at a fraction of the time and cost of other methods. The BGA uses arrays containing up to 1,024 features in each well of a standard microplate enabling 96-fold improvement in throughput. Hybridization setup and post-hybridization washes are performed with a robotic liquid handler, which reduces variability, processing time, and labor. Using the MessageAmp aRNA Amplification protocol, GeneXP's BGA typically delivers coefficients of variance below 15% with sensitivity to 1 mRNA spike molecule in 250,000 human transcripts.

A significant challenge to large scale expression analysis and profiling projects is sample preparation. For example, manual sample preparation using Ambion's Amino Allyl MessageAmp aRNA Amplification Kit is typically limited to 1020 aRNA preparations over a two-day period, or approximately 2040 samples per week. The rate limiting step for increasing sample q
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