Navigation Links
Automated Dissolution of Prednisone with the MultiDose G3

Jayne M. Brown, Lynn Jordan, Dale Von Behren
Caliper Life Sciences Corporation, Applications Laboratory, Hopkinton, Massachusetts, USA

Introduction

Prednisone is commonly used in laboratories as a dissolution bath calibrator. Manual dissolution is a very time intensive process; hence automation of this technique is highly desirable. In this study, the MultiDose G3 Workstation is used as a tool to automate the dissolution process by heating the media, filling vessels, dropping tablets, timing the dissolution process and sampling at the appropriate intervals. In addition to reducing the hands-on time involved in dissolution, the MultiDose G3 Workstation provides a 21CFR Part 11 compliant documentation of the automated dissolution process. The objective of this study is to determine the equivalency between manual dissolution sampling and analysis and automated dissolution sampling and analysis using the Prednisone calibrator tablet.

Materials and Methods

Instrumentation
All testing was performed using a MultiDose G3 Workstation (Caliper Life Sciences) and VK7000 dissolution bath (VanKel) with paddles and on-line MultiFill batch collection of samples in 16 x 100 test tubes. Off-line UV analysis was used to analyze the solutions with an 8453 UV/Vis Spectrophotometer (Agilent) with 1.0 cm cells.

Reagents and Standards
DI water was used for the dissolution media. The Prednisone standard Lot M was prepared according to the USP specification. The study was run using disintegrating USP Prednisone 10-mg tablets, Lot M.


The manually sampled solutions were filtered by drawing 10 mL of sample solution through a through a 35 mm VanKel Full Flow Filter. For the automated sampling, a Millipore Automation Certified 1.0 mm glass fiber filter is used.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Purified Single-Stranded M13 DNA for Automated Sequencing
2. Automated Fluorescent-Tag Cycle Sequencing
3. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
4. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
5. Agilent 2100 Bioanalyzer Automated Analysis System
6. Automated RNA Amplification for Arrays
7. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
8. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
9. Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents
10. DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
11. Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Automated Dissolution Prednisone with the MultiDose

(Date:9/14/2014)... when it comes to survival strategies. They often create ... for example during treatment with antibiotics. A biofilm is ... slime capsule consisting of sugar chains and "curli". ... the first time created a detailed three-dimensional image of ... the bacterial cell wall, a crucial step in the ...
(Date:9/12/2014)... magnet using an electric field (magnetoelectric memory [MEM] ... next-generation technology for information processing and storage. Multiferroic ... due to the coexistence of electric and magnetic ... spontaneous electric and magnetic polarizations is rare in ... the MEM effect. This article briefly reviews a ...
(Date:9/12/2014)... Advancing the front lines of ... and biological threats is the mission of an ... (WPI) and the Georgian National Academy of Sciences, ... and Security Programme of the North Atlantic Treaty ... Workshop series, the event is titled “Nanotechnology to ...
(Date:9/12/2014)... Mountain View, CA (PRWEB) September 12, 2014 ... its fundraising campaign on Indiegogo. , The film, ... Stephen Hawking, Buzz Aldrin, Shimon Peres, Ashton Kutcher, Will.i.Am, ... changing the world and there’s only one university in ... it to help change the lives of a billion ...
Breaking Biology Technology:New insights in survival strategies of bacteria 2Worcester Polytechnic Institute to Lead Scientific Workshop on Nanoscale Defenses for Biological and Chemical Threats 2Worcester Polytechnic Institute to Lead Scientific Workshop on Nanoscale Defenses for Biological and Chemical Threats 3Documentary Film that Follows Singularity University Students as They Change the World Launches its IndieGoGo Fundraising Campaign 2
... , , , , , , ... Amex: NBS ) ("NeoStem" or the "Company"), an international,biopharmaceutical company ... it priced a public offering of 5,000,000 shares of its common ... after underwriting,discounts and commissions and expenses, will be approximately $5,697,500 ...
... , , , SHENYANG, ... Inc. (Nasdaq:, SSRX ) ("3SBio" or "the Company"), a ... manufacturing and marketing,biopharmaceutical products, and Ascentage Pharma Group Corporation, ... strategic,alliance to research, develop and commercialize best-in-class targeted cancer,therapeutics ...
... simple one-step process that produces both n-type and ... use of the promising material for future electronic ... used to increase conductivity in graphene nanoribbons used ... (SOG) material to graphene and then exposing it ...
Cached Biology Technology:NeoStem Prices Public Offering of 5,000,000 Shares of Common Stock 2NeoStem Prices Public Offering of 5,000,000 Shares of Common Stock 3NeoStem Prices Public Offering of 5,000,000 Shares of Common Stock 43SBio and Ascentage Pharma Form Strategic Alliance to Research, Develop and Commercialize Cancer Therapeutics 23SBio and Ascentage Pharma Form Strategic Alliance to Research, Develop and Commercialize Cancer Therapeutics 33SBio and Ascentage Pharma Form Strategic Alliance to Research, Develop and Commercialize Cancer Therapeutics 4Single-step technique produces both p-type and n-type doping for future graphene devices 2Single-step technique produces both p-type and n-type doping for future graphene devices 3
(Date:9/15/2014)... the Department of Science and Technology in Society in ... Virginia Tech, has won a 2014 National Science Foundation ... and problems of creating a global nuclear emergency response ... Nuclear Power Plant in March 2011 was a turning ... Schmid said. Three of the plant,s six nuclear reactors ...
(Date:9/15/2014)... of the cheetah: The big predator is the fastest land ... just a few seconds. As it ramps up to top ... it reaches a full gallop. , Now MIT researchers have ... a robotic cheetah a sleek, four-legged assemblage of gears, ... its feline counterpart. The team recently took the robot for ...
(Date:9/15/2014)... no published account where hippopotamuses are demonstrably shown swimming ... water. But if they can,t swim, how did they ... accepted models for the methods, patterns, and timing of ... Crete, and Madagascar) may need to be reconsidered. , ... mainland are not currently supported by positive geological ...
Breaking Biology News(10 mins):'Nuclear disasters don't respect national boundaries' 2Run, cheetah, run 2Run, cheetah, run 3
... shift away from traditional coffee-growing techniques may be increasing ... that has swept through plantations in Central America and ... studies the disease. The current outbreak of coffee ... Mexico since the fungal disease arrived in the region ...
... stations could be drastically reduced by a new, energy-efficient ... releases it when exposed to sunlight. In a ... University and CSIRO scientists for the first time discovered ... of materials known for their exceptional capacity to store ...
... African-American women battling vitamin D deficiencies need the same ... to a recent study accepted for publication in The ... (JCEM). Although women with darker skin tones ... to measure Vitamin D levels, called 25-hydroxyvitamin D or ...
Cached Biology News:Modern growing methods may be culprit of 'coffee rust' fungal outbreak 2Modern growing methods may be culprit of 'coffee rust' fungal outbreak 3Carbon sponge could soak up coal emissions 2African-American, Caucasian women should take identical vitamin D doses 2