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Aurum Plasmid 96 Kit Tech Note, Rev A

a certain 96-well plasmid purification kit. When all kits seem to satisfy this requirement, other considerations come into play, such as plasmid purity and yield, usage requirements (handling and time), and cost. Sufficiently concentrated template is also an important requirement for some applications such as cycle sequencing. No other 96-well plasmid purification kit performed as quickly as Aurum plasmid 96, and Aurum was one of the 2 best performing kits in plasmid yield and concentration. In addition, Aurum requires a minimum of handling with its innovative binary plate configuration. No other kit produced better sequencing data than did Aurum plasmid 96. And with a cost of $1.56 per prep, Aurum plasmid 96 represents a cost-effective choice for high-throughput plasmid purification.


Acknowledgements
ABI PRISM 3100 DNA sequencing was performed by Davis Sequencing, LLC, Davis, CA, USA.


References
Birnboim HC and Doly J, A rapid alkaline extraction procedure for screening recombinant plasmid DNA, Nucleic Acids Res 7, 15131523 (1979)

Marko MA et al., A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powder, Anal Biochem 121, 382387 (1982)

Vogelstein B and Gillespie D, Preparative and analytical purification of DNA from agarose, Proc Natl Acad Sci USA 76, 615619 (1979)

Yang RCA et al., Elution of DNA from agarose gels after electrophoresis, Methods Enzymol 68, 176182 (1979)


* Costs based on 2001 US list prices.


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