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Aurum Plasmid 96 Kit Tech Note, Rev A

d 96 provided the same sequencing quality in less time, with fewer handling steps, and at a lower cost.

Wizard SV 96 Plasmid DNA Purification System
One of the best performers in plasmid yield from the 10 OD culture, the Wizard SV 96 kit did not perform as well as Aurum plasmid 96 with respect to average yield and concentration for the 4 OD culture, the density of bacteria obtainable in 96-well grow blocks. As with Aurum plasmid 96, Wizard SV 96 filtration and DNA binding were accomplished simultaneously, saving time. However, the required extra steps (e.g., second neutralization and manifold disassembly/reassembly) and longer incubations increased Wizard SV 96 prep time to 52 min compared to 40 min for Aurum. The Wizard SV 96 cost was $1.93 per prep. Aurum was faster and much less expensive than Wizard SV 96 while still giving high-quality plasmid DNA sequencing results.

Perfectprep Plasmid 96 Vac, Direct Bind Purification System
Aurum plasmid 96 gave higher average yields and concentrations than did Perfectprep for both 4 and 10 OD cultures, giving almost twice as much plasmid yield at more than twice the concentration. Because the Perfectprep kit had difficulty filtering the 10 OD samples (i.e., wells clogged), plasmid yields from these culture samples were lower than anticipated. Although Perfectprep gave reasonable sequencing electropherograms, prep time was longer than for Aurum (50 min vs. 40 min). Additionally Perfectprep kit handling was greater, since the plasmid binding plate needed to be placed inside the vacuum manifold.

Montage Plasmid Miniprep96 Kit
Among the other plasmid purification kits tested, Montage average yields fro
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