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Aurum Plasmid 96 Kit Tech Note, Rev A

the best plasmid yields and concentrations from the 4 OD culture of all kits, the Aurum plasmid 96 kit also had the handling advantages of the binary plate configuration, in which both lysate filtration and plasmid binding plates remain outside the vacuum manifold chamber. When lysate filtration was complete, the lysate filtration plate was simply detached and discarded, without having to disassemble the manifold. Moreover, no extra time was spent binding the plasmid to the plasmid binding plate; lysate filtration and DNA binding were accomplished simultaneously. Since lysate filtration took less than 5 min and washing lasted less than 1 min, the entire plasmid purification was completed within 40 min. Sequencing electropherograms were of excellent quality.

QIAprep 96 Turbo Miniprep Kit
The QIAprep 96 Turbo purification plates had one of the fastest rates of liquid passage through the plates of all kits tested, less than 1 min each for the filtration and 2 wash steps. User handling involved with this kit, however, was much more extensive than with Aurum plasmid 96, because of the QIAprep kits extra steps (e.g., washes) and the need to place the DNA binding plate inside the vacuum manifold. Repositioning of the DNA binding plate required that the manifold be disassembled and then reassembled, increasing handling. As a result, QIAprep required about 50 min to purify 96 plasmid DNA samples, compared to 40 min for Aurum. QIAprep samples were of sufficient concentration and purity to give good sequencing results, but purifying these samples was costly: QIAprep 96 Turbo cost $1.90 per prep, compared to $1.56 per prep for Aurum*. QIAprep delivered quality sequencing electropherograms, but Aurum plasmi
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Related biology technology :

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4. Generate High-Quality, Directional Plasmid cDNA Libraries
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7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Basic Plasmid DNA Isolation Protocol
9. RNA-free Plasmid DNA Isolation Protocol
10. Basic Plasmid DNA Isolation Protocol
11. RNA-free Plasmid DNA Isolation Protocol
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