Most average A260/A280 ratios for the 6 kits were between 1.75 and 1.85; exceptions included the ratio for the plasmid purified with Montage from the 10 OD culture, and the ratios for Concert(96) samples, which were both inordinately low. Aurum plasmid 96 had average A260/A280 ratios of 1.84 and 1.83 for 4 and 10 OD cultures, respectively. Table 2 lists the average A260/A280 ratios for all 6 kits and for both bacterial densities processed.
Agarose Gel Electrophoresis
When plasmid samples isolated from the 4 OD culture were run on an agarose gel, Aurum plasmid 96 samples were confirmed to be more concentrated than those purified with Concert(96), Perfectprep, or Montage (Figure 2). One lane containing a Concert(96) sample produced significant UV fluorescence in the well, indicating possible genomic DNA contamination. There was no indication of RNA contamination or degradation of plasmid in any sample run on a gel.
ABI PRISM 3100 DNA
Sequencing All kits produced DNA with sequencing reads of at least 600 bases without any ambiguities. Aurum plasmid 96 sequencing reads (Figure 3) had among the longest usable read lengths.
Aurum Plasmid 96 Kit