Navigation Links
Aurum Plasmid 96 Kit Tech Note, Rev A

agar plate and incubated overnight at 37C with shaking at 250 rpm. LB-ampicillin medium (500 ml) supplemented with 2% glycerol was then inoculated with 500 l of the starter culture and incubated overnight at 37C with shaking at 250 rpm. Culture density was measured by taking an absorbance reading of a 20-fold dilution of the culture at λ = 600 nm.

Plasmid Purification
Samples of a 4 OD culture were transferred to each well in columns 16 of six 96-well grow blocks. Similarly, samples of a 10 OD culture were transferred to each well in columns 712. The grow blocks were then centrifuged at 1,500 x g for 10 min, the medium decanted, and the grow blocks blotted on paper towels to remove residual medium. Plasmid pCMV was subsequently purified from the bacteria using a different manufacturers 96-well plasmid purification kit for each grow block and following each kits protocol. For all kits, the plasmid DNA was eluted or resuspended in 80 l of the appropriate elution buffer. Plasmid DNA concentrations and quality were measured using UV spectrophotometry. All samples from the 4 OD culture were run on agarose gels to confirm purity and concentration of the plasmid. For each manufacturers kit, 5 of the samples purified from the 4 OD culture were sequenced on an Applera ABI PRISM 3100 capillary array sequencer, and the resulting electropherograms were analyzed for ambiguous counts within the first 600 bases to assess relative comparative read lengths.


Results
Plasmid Yields and Concentrations
With respect to overall plasmid DNA mass yield, the Aurum plasmid 96 kit was one of the 2 best performers, giving the highest yield of all kits with the 4 OD culture
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
2. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
3. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
4. Generate High-Quality, Directional Plasmid cDNA Libraries
5. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
6. New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways
7. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
8. Basic Plasmid DNA Isolation Protocol
9. RNA-free Plasmid DNA Isolation Protocol
10. Basic Plasmid DNA Isolation Protocol
11. RNA-free Plasmid DNA Isolation Protocol
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Aurum Plasmid Kit Tech Note Rev

(Date:8/21/2014)... 2014 Ontotext S4 , The ... Ontotext . Now the same enterprise hardened text ... and unstructured data is available to start-ups and mid-size ... technology. Organizations that do not have resources to evaluate ... use S4 since there is no need for on ...
(Date:8/21/2014)... SoundConnect , a unified communication ... of the nation’s Fastest Growing Private Companies by ... consecutive year. Inc. magazine today ranked SoundConnect NO. ... exclusive ranking of the nation's fastest-growing private companies. ... the most important segment of the economy—America’s independent ...
(Date:8/20/2014)... , Aug. 20, 2014 Clintrax Global, ... located in Raleigh, NC , ... as Vice President of Administration. ... oversee all corporate processes, including their alignment with ... Global, Brad worked as an HR executive, specializing ...
(Date:8/20/2014)... it that American Indians largely were wiped out by ... tuberculosis brought to the New World by European explorers., ... people lived in the Americas shortly before Europeans arrived, ... European diseases., But new research led by anthropological geneticists ... of the University of Tubingen in Germany indicates the ...
Breaking Biology Technology:Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 2Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 3Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 4SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 2SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 3Clintrax Global, Inc. Announces Addition to Executive Team 2Unusual discovery leads to fascinating tuberculosis theory 2Unusual discovery leads to fascinating tuberculosis theory 3
... Editor's note: This is the second of a two-part opinion piece ... M adison, Wis. - The environmental ... evidence that ethanol increases nitrous oxides and ozone. These studies were ... Environmental Protection Agency , the California Air Resources Board ...
... - NimbleGen Systems, Inc. , a supplier ... new chief financial officer with experience managing large entrepreneurial ... years of experience in capital raising, finance, and general ... estate, and diversified manufacturing. , ,In a statement released ...
... Wisconsin Department of Agriculture, Trade and Consumer Protection ... University of Wisconsin-Milwaukee for research on sustainable and ... , ,The research project, headed by Sarah Gong, ... to strengthen the state's plastics industry and encourage technology ...
Cached Biology Technology:Last ethanol article you'll ever need to read, Part II 2Last ethanol article you'll ever need to read, Part II 3Last ethanol article you'll ever need to read, Part II 4NimbleGen names CFO with capital-raising past 2UWM receives state funding for plastics research 2
(Date:8/21/2014)... smog-producing toxins in past decade, GTA still violates ... that while the Greater Toronto Area (GTA) has ... to smog, the city continues to violate the ... can cause or aggravate health problems such as ... a set of complex photochemical reactions involving volatile ...
(Date:8/21/2014)... fungus linked to dandruff, eczema and other itchy, flaky ... further global reachesincluding Hawaiian coral reefs and the extreme ... A review in the scientific journal PLOS Pathogens ... fungi of the genus Malassezia in light ... from around the world. , University of Hawai,i at ...
(Date:8/21/2014)... the Fram Strait is a key region in the global ... Greenland and Svalbard warm Atlantic water flows to the north ... Arctic water masses and sea ice push their way out ... of the Atlantic water cools here on its way to ... the water caused in this manner drives the global band ...
Breaking Biology News(10 mins):Despite a significant reduction in smog-producing toxins, the Greater Toronto Area still violates Canada's standards for ozone air pollution 2From dandruff to deep sea vents, an ecologically hyper-diverse fungus 2Arctic sea ice influenced force of the Gulf Stream 2Arctic sea ice influenced force of the Gulf Stream 3
... children living in communities where they do not receive ... given supplements, even if they are HIV-infected, according to ... Health and other institutions. , Previously, it was not ... in children by increasing their viral load. Zinc supplementation ...
... Many baffled parents have wondered whether their teenagers would ... have never really quite understood just how an organism ... growth should cease. , Now University of Washington biologists ... fly, have discovered an organ that assesses the size ...
... provides definitive answers and ways to repair lives , A ... of radiation exposure from the Chernobyl nuclear power plant (NPP) ... than 100 scientists has concluded. , As of mid-2005, however, ... from the disaster, almost all being highly exposed rescue workers, ...
Cached Biology News:Zinc supplements safe for HIV-infected children 2Researchers find gland that tells fruit flies when to stop growing 2Researchers find gland that tells fruit flies when to stop growing 3Chernobyl: The true scale of the accident 2Chernobyl: The true scale of the accident 3Chernobyl: The true scale of the accident 4Chernobyl: The true scale of the accident 5Chernobyl: The true scale of the accident 6Chernobyl: The true scale of the accident 7Chernobyl: The true scale of the accident 8Chernobyl: The true scale of the accident 9Chernobyl: The true scale of the accident 10Chernobyl: The true scale of the accident 11Chernobyl: The true scale of the accident 12Chernobyl: The true scale of the accident 13Chernobyl: The true scale of the accident 14Chernobyl: The true scale of the accident 15Chernobyl: The true scale of the accident 16
Mouse monoclonal antibody to ST8SIA2 - ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2...
... Object Parametric Analyzer and Sorter) large particle ... advantages of flow cytometry to applications that ... automated analysis and sorting. Our instruments ... traditional flow cytometers, but are designed to ...
Mouse polyclonal antibody raised against a partial recombinant AGTRAP. NCBI Entrez Gene ID = 57085...
... raised against a partial recombinant ... SF3B2 (NP_006833, 592 a.a. ... protein with GST tag. ... NM_006842 Protein ...
Biology Products: