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Assessing Gene Function with siRNA Libraries


Cases where changes in cell proliferation do not correlate with aberrant mitotic index warrant follow-up analysis of necrosis and apoptosis as likely causes. With this in mind, Cenix has recently developed a multiplex assay monitoring cell proliferation, mitotic index, necrosis, and apoptosis all within one screening experiment (the Cenix Oncology Multiplex-1; contact Cenix for more details at info@cenix-bioscience.com). Using this and other basic and/or disease-focused RNAi assays, Cenix is further investigating the roles of kinases and other genes covered by Ambion's Silencer siRNA libraries.


Acknowledgments
The data described in this article were provided by Ambion's partner and collaborator, Cenix BioScience GmbH. Special thanks to Andrew Walsh and Lisa Koski for siRNA design and bio-informatics support.


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