Navigation Links
Assessing Gene Function with siRNA Libraries

48 hours after siRNA transfection as monitored by real-time PCR. For each kinase siRNA, target mRNA levels were reduced by 70% or more as compared to the levels of mRNA obtained after transfection with the negative control siRNA.

Figure 1. mRNA Silencing by 178 Kinase siRNAs from the Silencer Kinase siRNA Library. HeLa cells were plated at approximately 8000 cells per well in 96 well plates. Twenty-four hours later the cells were transfected in triplicate with each siRNA (100 nM). Forty-eight hours post-transfection, RNA was isolated, converted to cDNA, and analyzed by real-time PCR. Shown are the relative mRNA levels compared to cells transfected with a control scrambled siRNA (red bar, Silencer Negative Control #1 siRNA).


Effects on Cell Proliferation
Figure 2 demonstrates the effect of each siRNA on cell proliferation. In wells in which the negative control siRNA was transfected, there were approximately 1,250 cells/microscopic image after 48 hours (Figure 2). Inhibition of cyclin B1, which is known to be critical for initiation of mitosis, results in growth arrest. As expected, cell proliferation was dramatically inhibited by the siRNA targeting cyclin B1; there were <700 cells/microscopic image in wells transfected with this siRNA. Figure 2 also shows several kinase siRNAs that inhibit cell proliferation. This inhibition can result from a number of underlying causes that fall into three broad categories: effects causing cell necrosis, effects causing apoptosis, and effects causing cell cycle deregulation. Interestingly, a few kinase siRNAs appear to have a stimulatory effect on cell proliferation.

Fig
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Assessing UV damage of hair
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
4. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
5. Isolation to Functional Analyses
6. Precursor miRNAs for Successful miRNA Functional Studies
7. Using Validated siRNAs in Functional Genomic Assays
8. Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function
9. Whole Gel Eluter Purification of a Functional Multiprotein DNA Replication Complex, Rev A
10. Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing
11. Custom and library siRNA for efficient gene silencing
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Assessing Gene Function with siRNA Libraries

(Date:12/24/2014)... report expects global cell isolation and expansion market to ... carefully analyzed data about the vital drivers, restraints, trends, ... Copy of Report @ http://bit.ly/1yUxy0T , According ... will keep witnessing growth at an impressive CAGR during ... by rapid technological advancements, government funding and investments, and ...
(Date:12/24/2014)... -- PharmaBoardroom,s new report, , Healthcare ... for free download , digs deep into this vibrant ... the sector today. One area where ... developing a homegrown pharmaceutical manufacturing base, even if the government,s ... way off. A cursory comparison with neighboring Morocco ...
(Date:12/22/2014)... PA & Brussels (PRWEB) December 22, 2014 ... (CfPIE), the global leader of technical training across ... Society for Clinical Data Management (SCDM) to provide ... and certification programs —providing access to the more ... partnership extends SCDM members with 10% off when registering ...
(Date:12/22/2014)... GenomeDx Biosciences today announced that a new ... genomic test for prostate cancer, was able to predict ... radical prostatectomy without adjuvant therapy. Although rapid metastasis in ... tumor genomics to identify these men who have a ... advance. The study has been published online by ...
Breaking Biology Technology:The Cell Expansion Market is Expected to Reach $14.8 billion by 2019 - New Research Report by MarketsandMarkets 2The Cell Expansion Market is Expected to Reach $14.8 billion by 2019 - New Research Report by MarketsandMarkets 3PharmaBoardroom Releases New Algeria Pharmaceuticals Report 2PharmaBoardroom Releases New Algeria Pharmaceuticals Report 3CfPIE Announces Partnership with the Society for Clinical Data Management 2CfPIE Announces Partnership with the Society for Clinical Data Management 3Study Published in European Urology Shows that the Decipher Prostate Cancer Classifier is Predictive of Rapid Metastasis in High-Risk Men who have had Prostate Surgery 2Study Published in European Urology Shows that the Decipher Prostate Cancer Classifier is Predictive of Rapid Metastasis in High-Risk Men who have had Prostate Surgery 3
... Aug. 30 Pressure,BioSciences, Inc. (Nasdaq: ... has been,issued its first patent by the ... Biomolecular Complexes" (Number,2,259,318), this issuance extends the ... patents previously issued in the United States,Japan, ...
... SOUTH SAN FRANCISCO, Calif., Aug. 30 VaxGen,Inc. ... filed with the,Securities and Exchange Commission (SEC) its ... December 31, 2006 and Quarterly Reports on Form ... reports can be found at the link titled,"SEC ...
... of the FDA,s,Cooperative Research and Development Agreement (CRADA) ... (FDA) on August 23, 2007, in a,presentation focusing ... at,the Third Annual Regulatory and Compliance Symposium -- ... was held at Harvard University. Helen Winkle, ...
Cached Biology Technology:Pressure BioSciences, Inc. Issued First Patent in Canada 2Pressure BioSciences, Inc. Issued First Patent in Canada 3VaxGen Files 2006 Financial Statements and Provides Cash Update 2FDA and Conformia Deliver Joint Presentation to Annual Regulatory and Compliance Symposium at Harvard 2FDA and Conformia Deliver Joint Presentation to Annual Regulatory and Compliance Symposium at Harvard 3
(Date:12/15/2014)... DUBLIN , Dec. 12, 2014 Research ... announced the addition of the "Global Facial ... http://photos.prnewswire.com/prnh/20130307/600769 ... the identification of individuals. Facial recognition system measures ... as nose, jaw edges, mouth, and the distance ...
(Date:12/11/2014)... ST. PAUL, Minn. , Dec. 10, 2014 ... in wireless physiologic monitoring, has released a new ... changing needs of preclinical toxicology researchers. M series, ... to help toxicologists collect the best possible physiologic ... studies. Adding functional endpoints to toxicology ...
(Date:12/10/2014)... CIE San Diego has launched an innovative ... tissue that enhances care coordination among social service and ... information; earned a second $1 million grant from the ... aging in community and; will be recognized as a ... 4-6p. CIE San Diego today announced ...
Breaking Biology News(10 mins):Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3New telemetry implants expected to change how large animal toxicology studies are conducted 2Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 2Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 3Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 4
... some of North Africa,s major droughts of centuries past, ... the year 1179. The first multi-century drought reconstruction ... severe droughts during the 13th and 16th centuries and ... An international research team figured out northwest Africa,s climate ...
... Spanish . A new model for manipulating vitamin ... Service (ARS) scientists who say it could help establish just how ... optimal growth and health. Newborn dairy calves get crucial vitamin ... the first few days after birth. Later, the neonatal calf ...
... 25, 2010An insightful and in-depth presentation of the most ... pediatric interstitial lung disease (ILD) is presented in a ... Pediatric Allergy, Immunology, and Pulmonology (PED), a peer-reviewed ... ). The entire issue is available free online. ( ...
Cached Biology News:20th century one of driest in 9 centuries for northwest Africa 220th century one of driest in 9 centuries for northwest Africa 3Model developed for manipulating vitamin D levels in calves 2Comprehensive overview of Children's Interstitial Lung Disease (chILD) in special issue of Pediatric Allergy, Immunology, and Pulmonology 2
... These handy dispensers save space ... hats, and other garb. They are ... small parts used in labs, processing ... static charges and the particles they ...
... Double staining is a technique used ... single tissue.(1-6) PicTure™-Double Staining Kit is a ... detecting two antigens simultaneously in the same ... sensitivity. The antigens can be identified using ...
... NOVOstar is an exciting product for cell-based ... assays required the use of expensive HTS ... scientists in the assay development stage. The ... cell-based assays as well as any other ...
... the DH-2000, but comes with a shutter (controlled ... 5 Hz) The DH-2000 Deuterium ... of deuterium and tungsten halogen light sources in ... produces a powerful, stable output from 215-200 nm. ...
Biology Products: