Navigation Links
Assess the In Vivo Activation of Signal Transduction Pathways with,,,PathDetect Reporting Systems

ed luciferase expression indicates that the signal pathway converging at the fusion trans-activator is affected by this compound or stimulus.

As with all transfection experiments, various conditions for experiments using the PathDetect systems should be optimized to achieve the best possible results. Some variables to consider for optimization include the choice of cell line, transfection method, and protocol. In particular, the amounts of the fusion activator plasmid and the expression vector for the gene of interest are the easiest variables to optimize and will have a dramatic effect on transfection results.9

Make New Trans-Activator Plasmids

To offer further research options, Stratagene has designed the pFA-CMV vector15 for use with the trans-reporting systems. Similar to all trans-activator plasmids, the pFA-CMV vector provides expression driven by the CMV promoter### and the capability for G418 selection of stable cell lines. However, the pFA-CMV vector features a multiple cloning site with 10 unique, conveniently arranged restriction sites for insertion of any activation domain sequence. By cloning a transcription factor of interest into the multiple cloning site of the pFA-CMV vector, researchers will have their own fusion trans-activator vectors for studying signaling pathways converging on transcriptional factors of interest.

Specificity of PathDetect Trans-Reporting Systems

A series of experiments (Figure 2) tested the activity and specificity of the trans-reporting systems. For each experiment, the fusion trans-activator plasmid and a known activator were cotransfected with the pFR-Luc reporter plasmid into 1.5 x 105 HeLa cells in 35-mm culture dishes. The total amount of DNA in each reaction was kept constant by adding quantities as needed
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats
2. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
3. A New Way to Assess siRNA Delivery Efficiency
4. Assessing Gene Function with siRNA Libraries
5. Fast, Accurate Assessment of siRNA-induced Gene Silencing
6. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
7. Assessing UV damage of hair
8. Caspase-3 Activation - An Indicator of Apoptosis in Image-Based Assays
9. Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)
10. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:10/22/2014)... (PRWEB) October 21, 2014 The ... the inorganic refrigerants market in Americas with analysis ... TOC of the Americas Inorganic Refrigerants Market report, ... provided. This also provides a glimpse of the ... is supported by various tables and figures. ...
(Date:10/22/2014)... WriteResult, LLC – a premier provider of ... a team of researchers from Yale University’s School of ... to provide electronic questionnaire data collection through use of ... in May, aims to evaluate the impact of a ... and participation in urban agriculture to impact their consumption ...
(Date:10/22/2014)... According to new market research ... (Consulting, Integration, Deployment & Support), by Service Model ... Private, Others (Hybrid & Community)) - Global Forecast ... the Cloud Professional Services Market into various sub-segments ... It also identifies the drivers and restraints for ...
(Date:10/22/2014)... , Oct. 22, 2014 Research ... "Organic Electronics Market by Material, by Application, Geography - ... to their offering. Organic electronics, also ... branch of material science which deals with small conductive ... organic because small molecules and polymers are carbon based, ...
Breaking Biology Technology:The Americas Inorganic Refrigerants Market is estimated to grow to $71.6 million by 2018 - New Report by MicroMarket Monitor 2The Americas Inorganic Refrigerants Market is estimated to grow to $71.6 million by 2018 - New Report by MicroMarket Monitor 3myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 2myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 4Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 5Global Organic Electronics (Semiconductor, Conductive, Dielectric and Substrate) Market - Analysis and Forecast (2014 - 2020) 2Global Organic Electronics (Semiconductor, Conductive, Dielectric and Substrate) Market - Analysis and Forecast (2014 - 2020) 3
... Sept. 22, 2011 PrimeraDx today announced that Albert Luderer ... President and CEO Matthew McManus commented:  "We are excited ...  We have just launched the ICE Plex System, which ... high multiplex, multi-modal qPCR, and Al brings many years of ...
... A team of researchers from Roche ( SIX: RO, ... and Early Development and Roche NimbleGen, reported inGenome Research (1) ... the cynomolgus monkey ( Macaca fascicularis ) and developed a ... in preclinical drug safety research. The cynomolgus macaque ...
... a rain, the cupped leaf of a pitcher plant becomes ... ants, spiders, and even little frogs. One by one, they ... a group of applied scientists at Harvard have created a ... blood and oil, and does so even under harsh conditions ...
Cached Biology Technology:PrimeraDx Appoints Albert A. Luderer to its Board of Directors 2Roche Researchers Sequence Complete Genome of Cynomolgus Monkey and Develop Novel Gene Expression Microarray for Drug Safety Assessment 2Roche Researchers Sequence Complete Genome of Cynomolgus Monkey and Develop Novel Gene Expression Microarray for Drug Safety Assessment 3Roche Researchers Sequence Complete Genome of Cynomolgus Monkey and Develop Novel Gene Expression Microarray for Drug Safety Assessment 4Slippery slope:Researchers take advice from a carnivorous plant 2Slippery slope:Researchers take advice from a carnivorous plant 3
(Date:10/16/2014)... respiratory tract infections and worldwide claims the lives ... and Ghent University have succeeded in developing a ... infection. , Xavier Saelens (VIB/UGent): "We discovered ... for the development of a novel approach to ... in numerous small children and elderly people." , ...
(Date:10/15/2014)... to assess the pandemic risk from strains of influenza ... not allow ourselves to become complacent that the most ... of scientists. , Influenza pandemics arise when a new ... develop widespread immunity – spreads in the human population. ... 100 years, the worst of which – the 1918 ...
(Date:10/15/2014)... increased resistance to bacterial pneumonia in female mice to ... , Females are naturally more resistant to respiratory infections ... shown that increased resistance to bacterial pneumonia in female ... 3 (NOS3). They also show that this enzyme is ... hormone estrogen. , The team, lead by Professor Lester ...
Breaking Biology News(10 mins):New perspectives for development of an RSV vaccine 2Scientists 'must not become complacent' when assessing pandemic threat from flu viruses 2
... linking prostate cancer to an obscure retrovirus derived from ... a forensic analysis of tissue samples and lab experiments ... confirmed. The connection, which scientists have questioned repeatedly ... than six years ago, when the telltale signature of ...
... wildlife ecologist Aldo Leopold was a meticulous taker of field ... at his Sauk County shack in Depression-era Wisconsin, Leopold routinely ... the first pre-dawn calls of the indigo bunting or robin, ... he heard, when he heard them, and details such as ...
... Md. (September 18, 2012)Propelled by rapid growth in the ... the American Physiological Society (APS; www.the-APS.org ), announced ... its editorial policy. The journal will continue to ... will dramatically increase the scope of papers accepted to ...
Cached Biology News:New study confirms erroneous link between XMRV and prostate cancer-contamination was the cause 2New study confirms erroneous link between XMRV and prostate cancer-contamination was the cause 3New study confirms erroneous link between XMRV and prostate cancer-contamination was the cause 4Aldo Leopold's field notes score a lost 'soundscape' 2Aldo Leopold's field notes score a lost 'soundscape' 3Physiological Genomics journal announces a major restructuring 2Physiological Genomics journal announces a major restructuring 3Physiological Genomics journal announces a major restructuring 4Physiological Genomics journal announces a major restructuring 5
Ca2+ Channel Ca gamma 2 Purified Anti-Mouse, Anti-Rat, Anti-Human clone Polyclonal, Isotype Rabbit Ig, 50 µl Consult technical datasheet for details....
Rabbit polyclonal antibody to NCOA2 [Agarose Immobilized]...
Monocarboxylate (lactate) transporter 2...
Mouse Anti-Human Kallikrein 2 (Hk2)...
Biology Products: