Navigation Links
Assess the In Vivo Activation of Signal Transduction Pathways with,,,PathDetect Reporting Systems

ed luciferase expression indicates that the signal pathway converging at the fusion trans-activator is affected by this compound or stimulus.

As with all transfection experiments, various conditions for experiments using the PathDetect systems should be optimized to achieve the best possible results. Some variables to consider for optimization include the choice of cell line, transfection method, and protocol. In particular, the amounts of the fusion activator plasmid and the expression vector for the gene of interest are the easiest variables to optimize and will have a dramatic effect on transfection results.9

Make New Trans-Activator Plasmids

To offer further research options, Stratagene has designed the pFA-CMV vector15 for use with the trans-reporting systems. Similar to all trans-activator plasmids, the pFA-CMV vector provides expression driven by the CMV promoter### and the capability for G418 selection of stable cell lines. However, the pFA-CMV vector features a multiple cloning site with 10 unique, conveniently arranged restriction sites for insertion of any activation domain sequence. By cloning a transcription factor of interest into the multiple cloning site of the pFA-CMV vector, researchers will have their own fusion trans-activator vectors for studying signaling pathways converging on transcriptional factors of interest.

Specificity of PathDetect Trans-Reporting Systems

A series of experiments (Figure 2) tested the activity and specificity of the trans-reporting systems. For each experiment, the fusion trans-activator plasmid and a known activator were cotransfected with the pFR-Luc reporter plasmid into 1.5 x 105 HeLa cells in 35-mm culture dishes. The total amount of DNA in each reaction was kept constant by adding quantities as needed
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats
2. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
3. A New Way to Assess siRNA Delivery Efficiency
4. Assessing Gene Function with siRNA Libraries
5. Fast, Accurate Assessment of siRNA-induced Gene Silencing
6. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
7. Assessing UV damage of hair
8. Caspase-3 Activation - An Indicator of Apoptosis in Image-Based Assays
9. Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)
10. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:12/22/2014)... Dec. 22, 2014  ( www.competitivehealth.com ) — Competitive ... medical bill review and advocacy service, has signed an ... WellCard Savings discount health services marketplace. 63% ... more than they expected to pay. As part of ... costs, WellCard Savings is pleased to offer medical bill ...
(Date:12/22/2014)... 22, 2014 Fertility Associates of Memphis ... Bank USA , a network of more than 20 ... partnership, patients have fast access to a national pool ... IVF . Fertility Associates of Memphis is the only ... treatment option. , Dr. William Kutteh , director ...
(Date:12/19/2014)... BioPlus Specialty Pharmacy (BioPlus), one ... of Nick Maroulis, Pharm.D. to the newly created position ... In this position, Dr. Maroulis will continue to manage ... of our multi-site pharmacies as the company expands. Dr. ... that time he has served in many different capacities ...
(Date:12/19/2014)... Dec. 19, 2014 Bina Technologies, Inc. (Bina, ... that they have been acquired by Roche (SIX: RO, ... privately held company that provides a big data platform ... (NGS) data for the academic and translational research markets. ... and will continue to focus on development of their ...
Breaking Biology Technology:WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 3BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2Bina Technologies is acquired by Roche. 2Bina Technologies is acquired by Roche. 3
... State University have developed a cheap and easy ... density. The researchers hope the findings will foster ... using nanowires, from nanoelectronics to nanosensors, especially on ... "Alignment is a critical first step for ...
... CAMBRIDGE, Mass., Feb. 28, 2011 Idenix Pharmaceuticals, Inc. ... in the discovery and development of drugs for the ... results for the fourth quarter and year ended December ... cash equivalents totaled $46.1 million. ...
... Feb. 28, 2011 Verenium Corporation (Nasdaq: ... specialty enzymes, today announced that Terminal 6, a major ... begun use of Verenium,s Purifine enzymatic degumming process at ... enzymatic degumming is a novel process that significantly increases ...
Cached Biology Technology:Stretched rubber offers simpler method for assembling nanowires 2Idenix Pharmaceuticals Reports Fourth Quarter and Year End 2010 Financial Results 2Idenix Pharmaceuticals Reports Fourth Quarter and Year End 2010 Financial Results 3Idenix Pharmaceuticals Reports Fourth Quarter and Year End 2010 Financial Results 4Idenix Pharmaceuticals Reports Fourth Quarter and Year End 2010 Financial Results 5Idenix Pharmaceuticals Reports Fourth Quarter and Year End 2010 Financial Results 6Idenix Pharmaceuticals Reports Fourth Quarter and Year End 2010 Financial Results 7World's Largest Soybean Processing Industrial Complex Converts Half of Capacity to Usage of Verenium's Purifine® PLC Enzymatic Degumming Process Engineered and Installed by Alfa Laval 2World's Largest Soybean Processing Industrial Complex Converts Half of Capacity to Usage of Verenium's Purifine® PLC Enzymatic Degumming Process Engineered and Installed by Alfa Laval 3World's Largest Soybean Processing Industrial Complex Converts Half of Capacity to Usage of Verenium's Purifine® PLC Enzymatic Degumming Process Engineered and Installed by Alfa Laval 4
(Date:12/17/2014)... N.C. , Dec. 16, 2014 Valencell, ... licensed its PerformTek biometric technology to industry leaders such ... highly accurate, clinically validated, biometric wearable products. These products ... 6-9 in Las Vegas . ... technology is accurate, flexible and robust – with the ...
(Date:12/11/2014)... 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/twhjll/biometrics_market ) has announced the addition of ... 2015-2019"  report to their offering. ... advances in technology. With continuous advances in technology, ... the latest standard that meets the needs and ...
(Date:12/10/2014)... You,ve been here before: you desperately need to ... password, site key or the answer to your secret question. ... Today, Hoyos Labs , a digital ... finally put an end to the frustration that comes with ... 1U leverages a user,s smartphone to acquire his or her ...
Breaking Biology News(10 mins):Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3
... marine invertebrates worms, sponges, jellyfish - have the ... than two cells: a photoreceptor cell and a pigment ... suggested by Charles Darwin as the first eyes to ... allow the animal to sense the direction of light. ...
... Chemical Society (ACS) News Service Weekly PressPac with news from ... .,Please credit the individual journal or the American Chemical Society ... FOR IMMEDIATE RELEASE EXCEPT ARTICLE #5, which is embargoed for ... ARTICLE #1 FOR IMMEDIATE RELEASE Microcapsules act as ...
... a few extra treats this holiday season? Soon, your ... your exercise routine and keep the pounds off over ... to keep track. Researchers at the University of ... applications, dubbed UbiFit and UbiGreen, to automatically track workouts ...
Cached Biology News:Uncovering secrets of life in the ocean 2American Chemical Society's Weekly PressPac -- Nov. 19, 2008 2American Chemical Society's Weekly PressPac -- Nov. 19, 2008 3American Chemical Society's Weekly PressPac -- Nov. 19, 2008 4American Chemical Society's Weekly PressPac -- Nov. 19, 2008 5American Chemical Society's Weekly PressPac -- Nov. 19, 2008 6American Chemical Society's Weekly PressPac -- Nov. 19, 2008 7American Chemical Society's Weekly PressPac -- Nov. 19, 2008 8Track your fitness, environmental impact with new cell phone applications 2Track your fitness, environmental impact with new cell phone applications 3
Anti-Wnt-2 Polyclonal Antibody Description: 100 g purified goat polyclonal antibody. Reacts with human/mouse/rat. Tested in Western blot/IP/IHC. Research Focus: signal transduction Storage...
... custom microplate coating using advanced automated high throughput microplate processing ... We can also accommodate virtually any lot size of the ... , 8 and 12 well ... 384 well microplate, ...
...
... Mynox is the first ... by killing them, and not ... is the only anti-mycoplasma agent ... clean virus stocks directly. The ...
Biology Products: