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Assess the In Vivo Activation of Signal Transduction Pathways with,,,PathDetect Reporting Systems

ances in signal transduction.

With advances in molecular biology techniques and cell biology research, hundreds of genes encoding receptors, protein kinases, phosphatases, and other regulatory proteins have been cloned. Many of these gene products are implicated in signal transduction pathways. Unfortunately, standard cloning methods do not offer functional information about these newly identified proteins. Methods are required that will allow the in vivo function or activity of these proteins to be determined.

Stratagenes PathDetect in vivo signal transduction pathway reporting systems are designed to specifically, rapidly, and conveniently assess the in vivo activation of certain signal transduction pathways. At this time, Stratagene offers two types of pathway reporting systems, the PathDetect trans- and cis-reporting systems, each applicable to specific research needs. Together, these systems give researchers comprehensive tools to evaluate the role or involvement of an uncharacterized gene product, growth factor, or drug candidate in complex signal transduction interactions.

Trans-Reporting Systems: Assess Activation of Specific Pathways

Figure 1

Many extracellular signals modulate cellular activities by inducing intracellular signal transduction pathways that lead to activation of specific protein kinases.1,2,3,4 In turn, these kinases phosphorylate and activate specific transcription factors. Measuring the activation or inactivation of key kinases is one method for assessing the in vivo effects of a newly discovered gene product or drug candidate on the signaling molecules along these pathways. Each PathDetect trans-reporting system5 (Figure 1) is designed for studying the activation of specific pathways and
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