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Assess the In Vivo Activation of Signal Transduction Pathways with,,,PathDetect Reporting Systems


A review of products for signal transduction research

Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems

Li Xu Tim Sanchez Mary Buchanan Chao-Feng Zheng
Stratagene

Signal transduction is essential to many cellular processes, such as growth, differentiation, and coordination of cellular activities. One approach for determining the in vivo function of newly identified gene products, growth factors, and drug candidates is to identify the changes they exert on various signal transduction pathways. Since many signal transduction pathways converge at a trans-acting transcription factor or a cis-acting enhancer element, Stratagene offers the PathDetect in vivo signal transduction pathway reporting systems. The PathDetect trans-reporting systems, with specific fusion trans-activators, assess the activation of specific kinases and the corresponding signal transduction pathways by a gene product or extracellular stimuli. The PathDetect cis-reporting systems assess the activation of transcriptional factors acting on the cis-elements and provide preliminary clues on the function of the gene product or the effects of the stimulus. Together, these systems provide researchers with comprehensive and unique tools for studying the complex interactions of signal transduction.

Cells respond to environmental cues or other cells through numerous proteins or modifications of these proteins. These responses are the basis of signal transduction, whereby extracellular or intercellular signals are conveyed between cells by delicate networks of signaling molecules. By studying the intricacies of signal transduction reactions, we will learn about many aspects of cellular growth, differentiation, and coordination of cellular functions. In fact, the cause of many human diseases is attributed to disturb
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