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Array Analysis: The Basics

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For membrane array analysis, a file of the data is generated by phosphoimaging and that file is then analyzed using software. The software will correlate spots to genes and can compare spot intensities for differential expression studies.

Glass array data is treated in much the same way, but the image's fluorescence is scanned and the software allows detection of each samples' fluorescence individually or simultaneously for analysis. Most software packages can analyze several arrays simultaneously.


Validation
Differences in expression of specific sequences are often validated by another method of analysis such as RT-PCR, Northern analysis or nuclease protection assays. These same methods can be used for relative or absolute quantitation of specific messages of interest identified by array analysis.


Useful Web Sites to Visit

Academic Sites
The Brown Lab

Other Sites
http://www.gene-chips.com/
http://www.microarrays.org/


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