Navigation Links
Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C

fer without affecting the binding of the proteins to PVDF. The results are consistently clean, easy-to-read blots. The physical strength of Immun-Blot PVDF membrane means that it will not crack or tear during common handling, and will hold up under repeated stripping and reprobing applications.


Sequi-Blot PVDF Membrane
For protein sequencing applications, Sequi-Blot PVDF membrane is the best choice due to its extremely high protein binding capacity of 170200 g/cm2. This is the original Bio-Rad PVDF membrane, designed to withstand the conditions of N-terminal sequencing while providing the binding capacity to sequence even low-abundance samples. The table shows the increased protein recovery possible with Sequi-Blot PVDF membrane. Recovery of blotted proteins is typically in the range of 80100% of the initial sample load, resulting in higher initial coupling yields.

The binding efficiency of Sequi-Blot PVDF membrane is illustrated in Figure 3. This experiment showed that Sequi-Blot PVDF membrane is able to retain proteins that transfer through a competitors product.


Bibliography
Hulmes JD et al., Strategies for microcharacterization of proteins using direct chemistry on sequencer supports, in Techniques in Protein Chemistry (T Hugli, ed) Academic Press, San Diego, 716 (1989)

Matsudaira P, Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes, J Biol Chem 262, 10,035 10,038 (1987)

Speicher DW, Microsequencing with PVDF membranes: efficient electroblotting, direct protein adsorption and sequencer program modifications, in Techniques in P
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Direct On-Membrane MS/MS Analysis using the Chemical Printer (CHIP-1000) and AXIMA-QIT
2. Imaging Chemiluminescent Western Blots
3. Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A
4. Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
5. Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
6. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
7. DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell
8. A New C-Terminal GST Vector for Protein Production in S. pombe
9. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
10. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
11. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
Post Your Comments:
(Date:7/29/2015)... 29, 2015 Research ... addition of the "Global Biosimilars Market, 2015 ... The Global Biosimilars Market, 2015 - 2025, ... growing biosimilars market. With the blockbuster biologics losing ... costs, biosimilars are being viewed as viable substitutes ...
(Date:7/29/2015)... Inc., an early stage biotech startup based on perinatal ... the Musculoskeletal Transplant Foundation of Edison NJ ... round of development of the proprietary AmnioCept™ product line ... AmnioChor,s technology allows cryopreservation of the amniotic membrane of ... those tissues. Amnion is a well-established source of multipotent ...
(Date:7/29/2015)... 29, 2015  Pfenex Inc. (NYSE MKT: PFNX) announced ... be released on Thursday, August 13, 2015, before the ... management will host a conference call to discuss the ... release outlining the financial results and business update will ... Please call 1-866-376-8058 (US) or 1-412-542-4131 (international) and reference ...
(Date:7/29/2015)... ... July 29, 2015 , ... The third Medical Innovation Impact Index ... , in particular, drug/device combinations. The current system received a score of 0 (range ... Patient Centered Innovation (iPCI) of Fairleigh Dickinson University’s ( FDU ) Rothman Institute ...
Breaking Biology Technology:Global Biosimilars Market to 2025: Monoclonal Antibodies (mAbs), Insulin, Interferons, G-CSF/ GM-CSF, Erythropoietins and Others 2Global Biosimilars Market to 2025: Monoclonal Antibodies (mAbs), Insulin, Interferons, G-CSF/ GM-CSF, Erythropoietins and Others 3The Musculoskeletal Transplant Foundation Agrees to Invest in AmnioChor 2Pfenex To Report Second Quarter 2015 Results and Provide Business Update on Thursday, August 13, 2015 2Third Medical Innovation Impact Index Issued by Fairleigh Dickinson University’s Initiative for Patient-Centered Innovation (iPCI) 2Third Medical Innovation Impact Index Issued by Fairleigh Dickinson University’s Initiative for Patient-Centered Innovation (iPCI) 3
... 31 Biomoda, Inc. (OTC,Bulletin Board: BMOD) ... of,early lung cancer detection testing and other ... Strategies LLC, a government consulting,firm focused on ... appropriations and development of advanced technologies. ...
... TARRYTOWN, N.Y., Oct. 31 Emisphere,Technologies, Inc. ... will move its,corporate headquarters from Tarrytown, New ... retain its scientific staff and laboratory,facilities in ... that focuses on a unique and improved ...
... IDIX ) announced today that it will report,its ... Tuesday, November 6,2007 after the U.S. financial markets close. ... release, Idenix,management will host a conference call at 4:30 ... company,s financial results for the third quarter,2007. To ...
Cached Biology Technology:Biomoda Hires Manzano Strategies LLC to Spearhead Government Affairs Efforts 2Emisphere Technologies, Inc. Moves Into New Corporate Headquarters 2Emisphere Technologies, Inc. Moves Into New Corporate Headquarters 3
(Date:7/8/2015)... -- The consumer products industry for innovative tech ... such as wearables, smart wallets & other smart technology are ... continues to rapidly grow.  Biometrics & Wearable Device Companies in ... Inc. (NASDAQ: GOOG ), Apple Inc. (NASDAQ: AAPL ... GPRO ) and Baidu Inc. (NASDAQ: BIDU ) ...
(Date:7/7/2015)... 2015 NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or ... commerce market announces a revised version of one of its first ... air on CNBC in New York , ... San Francisco metro areas.    ... ability to replace all the cards in your wallet and keep ...
(Date:7/2/2015)... 2015 Fingerprint Cards has received an ... FPC1155 from the distributor World Peace Industrial Group (WPI), part ... Asia . Deliveries are planned to take ... used by smartphone manufacturers in Asia . ... communicated revenue guidance of approximately 2,200 MSEK for 2015. ...
Breaking Biology News(10 mins):Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 2Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 3Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 4Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 5Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 6Wocket Smart Wallet Commercials Airing on CNBC to Showcase This Summer's Must- Have Tech Product 2Wocket Smart Wallet Commercials Airing on CNBC to Showcase This Summer's Must- Have Tech Product 3FPC Receives Order for Touch Fingerprint Sensors of 268 MSEK 2
... Elsevier, a world-leading provider of scientific, technical and ... Journal of Forensic Radiology and Imaging ( ... Society of Forensic Radiology and Imaging (ISFRI). ... radiology and imaging, JOFRI aims to provide ...
... -- MIT engineers have created genetic circuits in bacterial cells ... results, which are encoded in the cell,s DNA and passed ... the Feb. 10 online edition of Nature Biotechnology , ... biomanufacturing, or to program stem cells to differentiate into other ...
... of the protein endoglin in the blood vessels of ... to researchers at Karolinska Institutet and Lund University in ... The Journal of Experimental Medicine . Given that ... spread of cancer cells, the team suggests that drugs ...
Cached Biology News:Elsevier announces the launch of a new journal: Journal of Forensic Radiology and Imaging 2Cell circuits remember their history 2Cell circuits remember their history 3Tumor blood vessels prevent the spread of cancer cells 2
Mouse monoclonal antibody raised against a partial recombinant KRT4. NCBI Entrez Gene ID = KRT4...
Mouse monoclonal antibody raised against a partial recombinant MYO7A. NCBI Entrez Gene ID = MYO7A...
Mouse polyclonal antibody raised against a partial recombinant CD320. NCBI Entrez Gene ID = 51293...
... Protein Staining Solution stains proteins ... electrophoresis (detection limit >= 8 ng ... including washing and destaining processes can ... staining of gel (20 min), proteins ...
Biology Products: