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Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C

fer without affecting the binding of the proteins to PVDF. The results are consistently clean, easy-to-read blots. The physical strength of Immun-Blot PVDF membrane means that it will not crack or tear during common handling, and will hold up under repeated stripping and reprobing applications.


Sequi-Blot PVDF Membrane
For protein sequencing applications, Sequi-Blot PVDF membrane is the best choice due to its extremely high protein binding capacity of 170200 g/cm2. This is the original Bio-Rad PVDF membrane, designed to withstand the conditions of N-terminal sequencing while providing the binding capacity to sequence even low-abundance samples. The table shows the increased protein recovery possible with Sequi-Blot PVDF membrane. Recovery of blotted proteins is typically in the range of 80100% of the initial sample load, resulting in higher initial coupling yields.

The binding efficiency of Sequi-Blot PVDF membrane is illustrated in Figure 3. This experiment showed that Sequi-Blot PVDF membrane is able to retain proteins that transfer through a competitors product.


Bibliography
Hulmes JD et al., Strategies for microcharacterization of proteins using direct chemistry on sequencer supports, in Techniques in Protein Chemistry (T Hugli, ed) Academic Press, San Diego, 716 (1989)

Matsudaira P, Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes, J Biol Chem 262, 10,035 10,038 (1987)

Speicher DW, Microsequencing with PVDF membranes: efficient electroblotting, direct protein adsorption and sequencer program modifications, in Techniques in P
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