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Sequi-Blot PVDF Membrane
For protein sequencing applications, Sequi-Blot PVDF membrane is the best
choice due to its extremely high protein binding capacity of 170200 g/cm2.
This is the original Bio-Rad PVDF membrane, designed to withstand the
conditions of N-terminal sequencing while providing the binding capacity
to sequence even low-abundance samples. The table shows the increased
protein recovery possible with Sequi-Blot PVDF membrane. Recovery of blotted
proteins is typically in the range of 80100% of the initial sample load,
resulting in higher initial coupling yields.
The binding efficiency of Sequi-Blot PVDF membrane is illustrated in Figure 3. This experiment showed that Sequi-Blot PVDF membrane is able to retain proteins that transfer through a competitors product.
Bibliography
Hulmes JD et al., Strategies for microcharacterization of proteins using
direct chemistry on sequencer supports, in Techniques in Protein Chemistry
(T Hugli, ed) Academic Press, San Diego, 716 (1989)
Matsudaira P, Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes, J Biol Chem 262, 10,035 10,038 (1987)
Speicher DW, Microsequencing with PVDF membranes: efficient electroblotting,
direct protein adsorption and sequencer program modifications, in Techniques
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