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Antibodies for Studying NMDA Receptor Protein Expression and,,,Synapse-Specific Immunolabeling

B and NR2C. Stratagene is also offering Anti-Synapsin I, which can be used for studying synaptogenesis and synapse elimination in a variety of species. As an added convenience, a specific positive control is included with each antibody.

Anti-NMDAR2A recognizes a predominant band, migrating at approximately 180 kDa, from a rat brain homogenate preparation. In immunoprecipitation experiments, this antibody does not cross-react with NR2B or NR2C. Strong and specific immunolabeling was also demonstrated for Anti-NMDAR2B. Anti-NMDAR2C can be used as a pan antibody for all three proteins, as it labels a 140-kDa, NR2C band and 180-kDa, NR2A and NR2B bands.

REFERENCES
  1. Collingridge, G.L., et al. (1983) J. Physiol. (Lond.) 335: 33-46.

  2. Sun, W., et al. (1992) Proc. Natl. Acad. Sci. USA 89: 1443-1447.

  3. Moriyoshi, K., et al. (1991) Nature 354: 31-37.

  4. Monyer, H., et al. (1992) Science 256: 1217-1221.

  5. Laurie, D.J., and Seeburg, P.H. (1994) J. Neurosci. 14: 3180-3194.

  6. Monyer, H., et al. (1994) Neuron 12: 529-540.

  7. Snell, L.D., et al. (1996) Molecular Brain Research 40: 71-78.

  8. Stone, L., et al. (1994) J. Neurosci. 14: 301-309.

  9. Browning, M.D, et al. (1993) Biol. Psychol. 34: 529-535.

  10. Stone, L., et al. (1994) J. Neurosci. 14: 301-309.


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