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Antibodies for Studying NMDA Receptor Protein Expression and,,,Synapse-Specific Immunolabeling

R1.5 Significant functional diversity has been seen in recombinant systems with heterologous coexpression of various NR2 subunits and/or NR1 splice variants.6 However, little is known about the expression of the NMDAR subunit proteins in situ.

NMDA Antibodies

Figure 1

Stratagene introduces Anti-NMDAR2A, Anti-NMDAR2B and Anti-NMDAR2C antibodies suitable for studying the expression of specific NMDAR subunit proteins in different cell types and brain regions. These antibodies were raised against fusion proteins representing selected regions of the NR2A, NR2B and NR2C subunits that have no homology with other NMDA subunits or other known protein sequences.7 The specificity of Anti-NMDAR2A and Anti-NMDAR2B was established in immunoprecipitation and Western blot experiments of whole rat brain homogenates. Figure 1 shows that Anti-NMDAR2A recognizes a single band of approximately 180 kDa in NR2A-immunoprecipitated rat brain homogenates. Similar results were obtained with Anti-NMDAR2B (figure 2). A single band of 180 kDa is observed for NR2B-immunoprecipitated rat brain homogenate blotted with Anti-NMDAR2B. As expected, no cross-reactivity was seen for the NR2B immunoprecipitation blotted with Anti-NMDAR2A. Anti-NMDAR2C recognizes the expected band (approximately 140 kDa) of NR2C as well as the 180-kDa band of NR2A and NR2B (data not shown). Thus, this antibody can serve as a pan antibody for NR2A-C.

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