Navigation Links
Analyzing Trace-Level Impurities of a Pharmaceutical Intermediate Using ,,, an LCQ Deca Ion Trap Mass Spectrometer and the Mass Frontier ,,, Software Package

imental MS2 spectrum then it should have m/z values of M+2, M+52, and M+70 in the experimental MS2 spectra of the dihydro, hydroxychloro, and dichloro substituted impurities, respectively).

In Table 1, fragment ions that have been assigned the same mechanism of formation by Mass Frontier are highlighted with the same color. Taking this into account the table reveals that in all cases, fragment ions related by a common Mass Frontier predicted mechanism of formation either have the same experimental m/z value or have experimental m/z values that differ by the mass of the substituents depending on whether or not the fragment structure resulting from the Mass Frontier prediction contains the proposed site of substitution. This observation provides the main verification for the impurity structures that were proposed.



Conclusions

With the help of Data Dependent MS2 scanning with Dynamic Exclusion, three impurities of a proprietary pharmaceutical intermediate were detected at or below the 0.1% level and targeted for characterization. Isotopic patterns and mass differences provided the rationale for proposed structures that were
submitted to Mass Frontier for verification.

The structures proposed for the impurities were confirmed by a comparison of Mass Frontiers fragmentation predictions with the experimental MS2 spectra. The analysis supported the conclusion that all three of the impurities resulted from chemical substitutions on a specific double bond in the intermediate.

The characterization of impurities present in amounts as low as 0.1% of the parent abundance is
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Practical Applications of HyperDSC in a Pharmaceutical Laboratory
2. World Class Life Science and Pharmaceutical Companies Leverage DoveBid s Global Reach for Asset Management and Disposition
3. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
4. Oil-Free PCR Using the Hot Top Assembly
5. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
6. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
7. Using XL1-Red Mutator Strain to Generate Esterase Variants
8. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
9. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
10. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
11. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
(Date:9/2/2014)... ROCKVILLE, Md. , Sept. 2, 2014 /PRNewswire/ ... of novel anti-infective biologic and drug candidates targeting ... announced today positive results from its final preclinical ... Drug Administration (FDA) guidance, this bridging study was ... product candidate designed to prevent the devastating effects ...
(Date:9/2/2014)... - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the Company") today announced ... three large odour control systems for waste water treatment plants ... are consistent with the market resurgence in North ... CEO. "After a difficult period of restraint in our core ... sales growth. Our lower cost structure, technology improvements and supply ...
(Date:9/2/2014)... and Rocky Mount, NC (PRWEB) September 02, 2014 ... today the establishment of its 11th clinical research site: ... Research has assumed ownership and operation of the clinical ... Mount, NC, further expanding PMG’s presence in eastern North ... additional 130,000 patients. For the patients in Rocky Mount ...
(Date:9/2/2014)... 2, 2014  Spherix Incorporated (SPEX) -- an intellectual property development ... property, today announced that the United States Patent & ... in the month of August that are part of ... The issued patents are: , U.S.RE45,065 ... 19, 2014; and , U.S.RE45,095 issued August 26, ...
Breaking Biology Technology:Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 2Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 3Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 4Synthetic Biologics to Initiate Clinical Trials of SYN-004 in 4Q 2014 to Prevent Potentially Deadly C. difficile Infections 5PMG Research Announces the Formation of its Eleventh Site 2PMG Research Announces the Formation of its Eleventh Site 3United States Patent & Trademark Office Issues Three New Standard Essential Patents to Spherix 2
... is considered the spawning ground for the next wave of ... national angel market, a diversified range of opportunities await later-stage ... , the Center for Venture Research at the ... grew by nearly 11 percent in 2006, with total investments ...
... - For the second time this year, a tech-based ... for sale to the public. Called an initial public offering, ... the companies and investors who are directly involved, but to ... , a Madison-based biotechnology company, has filed papers with the ...
... experience high rates of failure. As a result, many ... partner. The result stifles innovation, growth, and a company's ... shedding the flawed processes of yesterday, and introducing methods ... promising incremental delivery of functional software. , , Evidence ...
Cached Biology Technology:National angel investment grew 11 percent in `06 2National angel investment grew 11 percent in `06 3Wisconsin IPOs are a harbinger of growing tech economy 2Wisconsin IPOs are a harbinger of growing tech economy 3Agile Revolution: A new era of software delivery 2Agile Revolution: A new era of software delivery 3Agile Revolution: A new era of software delivery 4
(Date:9/2/2014)... their propensity for eating anything, including each other, camel ... from North Carolina State University finds that non-native camel ... United States. , "The good news is that camel ... to humans," says Dr. Mary Jane Epps, a postdoctoral ... paper about the research. , The research stems from ...
(Date:9/2/2014)... lithographic limestone is well known as a result ... area (for example, Archaeopteryx). Now, for the first ... French equivalent of these outcrops - discoveries which ... water treader. , Despite the abundance of fossils ... been obtained from the Late Kimmeridgian equivalents of ...
(Date:9/2/2014)... touch a hot oven, you rapidly pull your hand ... in sensing and responding to such painful stimuli, they ... researchers have made a surprising discovery about the role ... and have built a structural model of the molecule. ... , may help direct new strategies to treat pain ...
Breaking Biology News(10 mins):Researchers find Asian camel crickets now common in US homes 2Exceptionally well preserved insect fossils from the Rhône Valley 2Surprising new role for calcium in sensing pain 2Surprising new role for calcium in sensing pain 3
... the Materials Genome Initiative (MGI) during his speech on ... the Advanced Manufacturing Partnership. The aim of MGI is ... twice the speed than is possible today." The related ... the White House Office of Science and Technology Policy ...
... the California Institute of Technology (Caltech) have been able, ... bacteria by transferring their DNA, and to measure the ... the early stages of infection by this type of ... cells targeted for infection, rather than the amount of ...
... relative of modern-day humans, enjoyed a diet of leaves, fruits, ... more wooded environment than is generally thought, a surprising find ... by an international team of researchers that includes a Texas ... the Department of Anthropology, says the new findings are in ...
Cached Biology News:Harnessing the Materials Genome Conference 2Harnessing the Materials Genome Conference 3Harnessing the Materials Genome Conference 4Harnessing the Materials Genome Conference 5The physics of going viral 2The physics of going viral 3The physics of going viral 4Diet of early human relative Australopithecus shows surprises, says Texas A&M researcher 2