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Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module

at compound concentrations below those which induce cell cycle arrest or cytotoxicity (Fig 6) allows determination of EC50 values for compounds in the in vitro micronucleus assay (Table 1).



Analysis of images acquired on IN Cell Analyzer 1000
Images acquired using IN Cell Analyzer 1000 may be analyzed using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module following conversion of images using IN Cell Translator Image Converter software (Fig 7). Converted images are analyzed using the analysis protocol described.


Conclusion
The IN Cell Analyzer 3000 Micronuclei Formation Analysis Module provides a rapid method (< 10 min for a 96-well plate) for analysis of in vitro micronucleus assays allowing automated screening of large numbers of compounds for genotoxicity. The software is compatible with standard assay protocols (1, 2) and may be used to analyze images acquired using the IN Cell Analyzer 1000 via image conversion to IN Cell Analyzer 3000 format.


References
1. Kirsch-Volders, M. et al. Report from the in vitro micronucleus assay working group. Mutat. Res. 540(2), 153–63 (2003).

2. Kirsch-Volders, M. et al. Report from the in vitro micronucleus assay working group. Environ. Mol. Mutagen. 35(3), 167–72 (2000).



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