Navigation Links
Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module

protocol
1. Seed CHO-K1 cells in imaging grade 96-well plates at 5000 cells/100 μl/well and incubate under standard tissue culture conditions for 24 h.

2. Prepare dilutions of test compounds in solvent. Prepare a 1-mg/ml stock solution of mitomycin C in PBS and sterile filter for use as a positive control. Add 100 μl of test compounds, solvent, and mitomycin C (use mitomycin C at 100-ng/ml final concentration) and incubate cells for 24 h.

3. Prepare a 3-mg/ml stock solution of cytochalasin B in DMSO. Dilute 1:100 in complete tissue culture medium and add 10-μl/well. Incubate cells for 24 h.

4. Remove media, wash cells once with PBS, and fix in ethanol for 30 min at room temperature.

5. Make 10-mg/ml stock solution of FITC in DMSO. Dilute 10 μl in 100-ml PBS and add 100-μl/well. Incubate for 30 min at room temperature.

6. Wash wells three times with PBS.

7. Stain cells with 5-μM Hoechst™ 33342 in PBS for 15 min at room temperature.

8. Image wells using excitation and emission settings for fluorescein and Hoechst 33342.


Analysis protocol
The IN Cell Analyzer 3000 Micronuclei Formation Analysis Module uses a series of operations based on user settings (Fig 2) to define nuclei, segregate mono-nucleate and binucleate cells, and define a search area for micronuclei (Fig 3). For full guidance on using the analysis module see the product manual.



1. Open run file in the IN Cell Analyzer 3000 analysis software.

2. Select a mitomycin C positive control well. Apply intensity threshold, erosion, and size filtration settings to identify nuclei in the blue (Hoechst) channel. Take care to exclude micronuclei from identified objects.

3
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
(Date:12/11/2009)... - Oncolytics Biotech Inc. (TSX:ONC, NASDAQ: ONCY ) ... of Dr. Alan Warrander to the role of ... ,, "Dr. Warrander will be responsible for the ... said Dr. Brad Thompson, President and CEO of ... of a Phase 3 trial examining REOLYSIN in ...
(Date:12/10/2009)... Microban International, Ltd., the global ... partnered with Sharp Imaging and Information Company ... to incorporate it,s third generation silver-based antimicrobial ... into the calculator keys during manufacture, Microban ... of damaging bacteria on the keys. ...
(Date:12/10/2009)... NEW YORK, Dec. 10 With the economy ... Company, Wall Street,s largest, fastest-growing small cap equity research ... of unique investment opportunities within the micro cap market ... on January 11, 2010 at the Grand Hyatt ... cap companies are overlooked and undervalued by much of ...
(Date:12/10/2009)... flow measurement, control, and level solutions, announced that ... Level Product Manager effective November 30, 2009. He ... penetration of the level measurement products around the ... Corp, a manufacturer of building materials, where he ... will report to Brooks Instrument Vice President Tim ...
Breaking Biology Technology:Oncolytics Biotech(R) Inc. Appoints Senior Vice President, Global Licensing and Business Development 2Oncolytics Biotech(R) Inc. Appoints Senior Vice President, Global Licensing and Business Development 3Microban Signs Exclusive Agreement with Sharp Electronics to Launch Calculators with New Microban(R) 3G Silver(TM) Antimicrobial Technology 2Technology, Biotech, Energy and Transportation Among the Industries to be Featured at Sidoti & Company's First Annual 2010 Micro Cap Conference 2Brooks Instrument Appoints Mike Bayda as Global Level Product Manager 2Brooks Instrument Appoints Mike Bayda as Global Level Product Manager 3
... webcast: , , What: Abbott,s exercise of its ... a subsidiary of Isis. Total acquisition price will be, ... December 17, 2008 at 8:30 a.m. ET / 5:30 a.m. PT, Where: ... Simply log onto our Web site listed, ...
... line with Jatropha World Asia 2009, scheduled 16-17 ... of interviews with top Jatropha decision makers have ... and answer session was held with Pradip Bhar, ... Magor Biofuel Limited. Offered by free download, this ...
... ... (PRWEB) December 17, 2008 -- Facilitating the entry of ... protocol analyzer, now updated to effortlessly examine not only the wireless ... modem, but also the interface between the device CPU and the ...
Other Biology Technology:Webcast Alert: Isis Pharmaceuticals Announces That Abbott Exercised Its Option to Acquire Ibis Biosciences 2Jatropha World 2009 Advance Access: Surprisingly Candid Interview Series Examines Biofuel Predictions Compared to Fossil Fuels 2Jatropha World 2009 Advance Access: Surprisingly Candid Interview Series Examines Biofuel Predictions Compared to Fossil Fuels 3Jatropha World 2009 Advance Access: Surprisingly Candid Interview Series Examines Biofuel Predictions Compared to Fossil Fuels 4Newly Available To Medical Device Manufacturers - Bluetooth Testing Equipment 2Newly Available To Medical Device Manufacturers - Bluetooth Testing Equipment 3
(Date:12/14/2009)... Pa., Dec. 14 MODA Technology Partners, ... (EM), utility and product testing for the Pharmaceutical ... 3.0, the company,s third generation mobile data acquisition ... organizations to further automate their regulated manufacturing processes. ... capabilities: ,, Fully-Configurable Test Methods: ...
(Date:12/13/2009)... may help to engineer plants that take advantage of ... finding could help to engineer crops that take advantage ... carbon dioxide they need for photosynthesis through microscopic breathing ... molecule of the gas gained, they lose hundreds of ... tighten to save water when CO 2 is ...
(Date:12/13/2009)... New Haven, Conn.A team led by Yale University ... in whole blood for the first time. Their ... online publication of Nature Nanotechnology , could ... of cancer and other diseases. , ... Professor of Engineering & Applied Science, and Tarek ...
Breaking Biology News(10 mins):MODA Announces Availability of Third Generation Mobile Data Acquisition Platform 2Newly identified enzymes help plants sense elevated CO2 and could lead to water-wise crops 2Newly identified enzymes help plants sense elevated CO2 and could lead to water-wise crops 3Scientists use nanosensors for first time to measure cancer biomarkers in blood 2Internationally Renowned Bariatric and Laparoscopic Surgeons To Meet in San Diego 5767 1Internationally Renowned Bariatric and Laparoscopic Surgeons To Meet in San Diego 5767 2BioMarin to Present at the Oppenheimer Healthcare Conference 5764 1BioMarin to Present at the Oppenheimer Healthcare Conference 5764 2PAREXEL Reports First Quarter Fiscal Year 2010 Financial Results 5761 1PAREXEL Reports First Quarter Fiscal Year 2010 Financial Results 5761 2PAREXEL Reports First Quarter Fiscal Year 2010 Financial Results 5761 3PAREXEL Reports First Quarter Fiscal Year 2010 Financial Results 5761 4PAREXEL Reports First Quarter Fiscal Year 2010 Financial Results 5761 5PAREXEL Reports First Quarter Fiscal Year 2010 Financial Results 5761 6PAREXEL Reports First Quarter Fiscal Year 2010 Financial Results 5761 7PAREXEL Reports First Quarter Fiscal Year 2010 Financial Results 5761 8PAREXEL Reports First Quarter Fiscal Year 2010 Financial Results 5761 9PAREXEL Reports First Quarter Fiscal Year 2010 Financial Results 5761 10
... new strategy developed in the Neonatal and Infant Feeding ... infants and other newborns with severe swallowing difficulties learn ... appearing in the February issue of the Journal ... Children,s were able to help 15 out of 20 ...
... N.C. Duke University Medical System researchers have discovered ... provide a new approach for future smoking-cessation products. ... Oliveira-Maia, M.D., Ph.D., a postdoctoral fellow of the Duke ... explore both the peripheral taste pathways activated by nicotine, ...
... NEW YORK The Damon Runyon Cancer Research ... researcher John Rinn, PhD, has been awarded a 2009 ... made to early-career researchers who are using "novel approaches ... member of the faculty at the Broad Institute in ...
Other Biology News:Crib-side studies help struggling newborns go home without feeding tubes 2Crib-side studies help struggling newborns go home without feeding tubes 3Nicotine activates more than just the brain's pleasure pathways 2BIDMC scientist John Rinn, Ph.D., receives Damon-Runyon Rachleff Innovation Award 2
Consists of 4 separate 100mM solutions (ATP,GTP,CTP and UTP pH 7.5) as sodium salts. 98% Pure by HPLC...
Collected from sexually mature, mixed sex, mixed breed cats. Physiochemical constituents are measured to ensure lot-to-lot consistency....
Tracker Datamatrix TRxD reads any 1D and 2D barcodes within 0.5 seconds....
Donor feline serum and plasma...
Biology Products: