Navigation Links
Analysis of a Verapamil Microsomal Incubation using Metabolite ID and Mass FrontierTM

using a FinniganTM LCQTM Advantage ion trap mass spectrometer equipped with a Finnigan Surveyor HPLC system. The mass spectrometer was operated in a data-dependent acquisition mode in which both MS and MS2 spectra were acquired without the need to specify parent masses. In this mode, the acquisition software probed the MS spectra in real-time on a scan-by-scan basis to select the two most intense parents ions for MS2 analysis. By analyzing both the first and the second most intense parent ions, information on co-eluting metabolites was obtained. Dynamic ExclusionTM was not employed in these analyses in order to avoid the possibility of missing modifications such as hydroxylations that can occur multiple times with closely spaced retention times. The ion source of the mass spectrometer was operated in the positive ion electrospray ionization mode. The HPLC separation was achieved using a 150 2.1 mm 5 μm BetaBasic 18 column (Thermo Electron) and the following HPLC gradient conditions:

Metabolite ID Analysis
Background subtraction is an optional step that was used for the verapamil example presented here. The background subtraction feature includes a scaling factor by which the control files intensity can be multiplied (to ensure complete background subtraction) and a time offset (to correct for any retention time drift between the source and the control file). Metabolite ID also provides a manual background subtraction feature that allows the user to specify a specific mass range and time range to be subtracted.

After the optional background subtraction, the appropriate data file is opened and the parent drugs of interest are specified as illustrated in Figure 1. Users can select from previously created drug lists or create new entries by pressing the new Item tab. The Modifications button allo
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
(Date:9/17/2014)... (PRWEB) September 17, 2014 JH ... California showroom located in Irvine. JH Technologies, a ... imaging systems, recently announced an expanded partnership with Leica ... leading microscopes and image analysis solutions will be featured ... 2nd. The new showroom facility is located within ...
(Date:9/17/2014)... 17, 2014 Intarcia Therapeutics, Inc. ... a study of the cost and predictability of non-adherence ... Annual Meeting of the European Association for the ... Christian Frois , Ph.D., of Analysis Group, Inc. ... planned Intarcia-sponsored, retrospective studies to characterize the prevalence of ...
(Date:9/17/2014)... Calif. , Sept. 17, 2014 ... molecular diagnostics company pioneering the field of molecular cytology, ... chief operating officer. Mr. Hall joined Veracyte ... company,s commercial entry into endocrinology. Since 2012, he has ... well as managed care and billing functions. As chief ...
(Date:9/17/2014)... 17, 2014 Escape Alert, LLC . filed ... which will alert owners if their pet has escaped and enable ... implanted microchip in the pet’s body. This is the first ... pets have been around for many years, they are passive until ... to track a pet’s location or alert the owner that the ...
Breaking Biology Technology:JH Technologies displays newest microscope imaging technology at Southern California showroom grand opening 2JH Technologies displays newest microscope imaging technology at Southern California showroom grand opening 3Intarcia Reveals New Insights At EASD Meeting Characterizing The Burden Of Poor Control And Non-Adherence To Anti-Diabetic Therapies 2Intarcia Reveals New Insights At EASD Meeting Characterizing The Burden Of Poor Control And Non-Adherence To Anti-Diabetic Therapies 3Intarcia Reveals New Insights At EASD Meeting Characterizing The Burden Of Poor Control And Non-Adherence To Anti-Diabetic Therapies 4Intarcia Reveals New Insights At EASD Meeting Characterizing The Burden Of Poor Control And Non-Adherence To Anti-Diabetic Therapies 5Veracyte, Inc. Announces Promotion of Christopher M. Hall To Chief Operating Officer 2Veracyte, Inc. Announces Promotion of Christopher M. Hall To Chief Operating Officer 3Veracyte, Inc. Announces Promotion of Christopher M. Hall To Chief Operating Officer 4Implantable Microchip for Pets with GPS Tracking - Patent Filed by Escape Alert, LLC. 2Implantable Microchip for Pets with GPS Tracking - Patent Filed by Escape Alert, LLC. 3
... by leading European authority on natural/ organic ... Oct. 6 /PRNewswire-FirstCall/ - Botaneco Specialty Ingredients,Inc. (Botaneco), ... that its second generation oleosome product, Hydresia(TM) G2, ... material, complying with,the standards for ecological and organic ...
... software and services company, today announced SPL for ... Documentum,s Documentum Compliance Manager (DCM) application. SPL ... solution and addresses the SPL Release 4 submission ... latest addition to Glemser,s family of pharmaceutical labeling ...
... contains many genes encoding proteins that are similar to those ... for only a small fraction the genome, and there are ... What are these sequences doing, and do we really need ... in Genome Research ( www.genome.org ), researchers have ...
Cached Biology Technology:Botaneco receives natural ingredient approval from ECOCERT(R) for Hydresia(TM) G2 2Botaneco receives natural ingredient approval from ECOCERT(R) for Hydresia(TM) G2 3Glemser Announces New Labeling Solution: SPL for DCM Addresses New SPL Release 4 Requirements 2Use it or lose it? Researchers investigate the dispensability of our DNA 2
(Date:9/17/2014)... MRSA and MSSA could be curbed by coating ... scaly surface of shark skin, according to research ... Resistance and Infection Control . , The study ... of human disease bacteria through touching, sneezes or ... of ridges formulated to resemble shark skin. The ...
(Date:9/16/2014)... (17 September, 2014)More than $100 trillion in cumulative ... annual carbon dioxide (CO2)a 40 percent reduction of ... if the world expands public transportation, walking and ... released by the University of California, Davis, and ... , Further, an estimated 1.4 million early ...
(Date:9/16/2014)... viruses that make us sick can take up residence ... sneeze, cough or other troublesome symptom, according to new ... Louis. , On average, healthy individuals carry about ... report online in BioMed Central Biology . The ... diversity of viruses in healthy people. , The ...
Breaking Biology News(10 mins):Sharks' skin has teeth in the fight against hospital superbugs 2Global shift away from cars saves US$100 trillion, eliminates 1,700 MT of CO2 pollution 2Global shift away from cars saves US$100 trillion, eliminates 1,700 MT of CO2 pollution 3Global shift away from cars saves US$100 trillion, eliminates 1,700 MT of CO2 pollution 4Global shift away from cars saves US$100 trillion, eliminates 1,700 MT of CO2 pollution 5Healthy humans make nice homes for viruses 2Healthy humans make nice homes for viruses 3
... BETHLEHEM, PA, March 17, 2009 -- Lehigh University ... awarded a $1.8 million grant from the National ... and Other Communication Disorders for his research entitled ... grant will allow Burger to build upon the ...
... between food, nutrition science, and health outcomes have been ... individuals and populations is a new factor that enriches ... the rapidly emerging intersection of nutritional science and genomics ... issue of OMICS: A Journal of Integrative Biology ...
... papilloma virus (HPV)-infections can lead to cellular changes in ... cancer. Surgical treatment of these pre-stages gives an increased ... HPV-vaccine can prevent pre-stages of cervical cancer, it may ... Norwegian study has calculated the benefits of HPV-vaccination. ...
Cached Biology News:Lehigh researcher awarded $1.8 million NIH grant 2Lehigh researcher awarded $1.8 million NIH grant 3Nutrigenomics -- developing personalized diets for disease prevention -- part 2 just published in OMICS 2HPV vaccine may prevent preterm births 2
Human Apolipoprotein B100 Affinity Purified Polyclonal Ab ENTREZ GeneID: 338...
Mouse Ameloblastin Biotinylated Affinity Purified PAb ENTREZ GeneID: 11698...
Human SOST Affinity Purified Polyclonal Ab...
Homo sapiens CDC23 (cell division cycle 23, yeast, homolog),...
Biology Products: