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Analysis of a Verapamil Microsomal Incubation using Metabolite ID and Mass FrontierTM

in these examples the reference compound for comparison will be the N-dealkylated metabolite of verapamil rather than verapamil itself.

Conclusion
The utility of the Metabolite ID and Mass Frontier software packages for identifying metabolites and elucidating their structures was demonstrated using a microsomal incubation of the drug, verapamil. Specifically, Metabolite ID quickly identified 17 different distinct types of modifications. It was shown that with Metabolite ID, a variety of different search tools can be employed to more completely characterize the metabolites in a sample. Mass Frontier was shown to be capable of assigning the specific sites of modification of metabolites using their MS2 spectra.


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