Navigation Links
Analysis of Phenolic Estrogen Mimics in River Water by Negative Ion Electrospray LC/MS/MS

Z. Yang, Varian, Inc.

Introduction

Bisphenol A (BPA) is a primary raw material used for the production of polyacrylates, epoxy resin, phenolic resin, and polycarbonate plastic. 4-Nonylphenol (4-NP) is a degradation product of alkylphenol ethoxylate detergents used in household and industrial cleaning products. Alkylphenol ethoxylate is also widely used as an additive in plastics that are used for food packaging and used as carriers in the application of pesticides to increase the absorption of these substances in the target organism.

BPA and 4-NP can leach from food containers and plastic tableware and can be ingested by humans through chronic exposure during eating and drinking. The chemicals have been found in drinking water supplies and wastewater.

Both BPA and 4-NP mimic the effect of natural estrogen hormones. This phenomenon raises the level of concern about the adverse health effects of these compounds on both humans and wildlife.

Instrumentation

Varian ProStar 430 AutoSampler

Varian ProStar 210 Isocratic Solvent Delivery Modules

Varian ProStar 510 Column Oven

Varian 1200L LC/MS equipped with ESI source

Materials and Reagents

Bisphenol A (Part No. 442840) and 4-Nonylphenol (Part No. 442873) supplied by Sigma-Aldrich Corp. (St. Louis, Missouri, U.S.A.).

All other chemicals are reagent grade or HPLC grade.

Sample Preparation

Samples were provided by a water company in Japan. The concentration of 4-NP and BPA were approximately 10 to 100 ng/mL and 1 to 10 ng/mL, respectively.

The samples were extracted with dichloromethane. The extracts were dried by a N2 stream and restored in the same volume of methanol/water (1:1, v/v) prior to analyses.

Results and Discussion

The LC method used a 12 minute cycle time. The data was acquired in SRM mode of two time segments (Figure 1).

Each standard calibration curve was generated using 6 points corresponding to a concentration range of 0.5 pg/μL* to 100 pg/μL (Figure 2). The linearity of the detector response was excellent with R2 0.999 for both BPA and 4-NP.

The method can quantitatively analyze both BPA and 4-NP at a level of 500 fg/μL in solution (Figure 1, bottom chromatogram). The limit of detection (LOD) is estimated to be about < 200 fg/μL in solution. The typical SRM chromatogram of water extract is given in Figure 1 (top chromatogram).

Conclusion

Estrogen mimics are included in a long list of chemicals targeted by various governmental and environmental groups. Complex matrices require instrumentation with good sensitivity and selectivity. In this evaluation, the use of a Varian 1200L LC/MS/MS system demonstrates how sensitive and selective tandem mass spectrometry is in the analyses of phenolic estrogen mimics. The 1200L can quantitatively analyze BPA and 4-NP at 500 fg/μL.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/25/2016)... ... May 25, 2016 , ... The ... fracture-specific plating options designed to address fractures of the distal tibia and fibula. ... The Acumed Ankle Plating System 3 is composed of seven plate families that ...
(Date:5/25/2016)... ... ... Scientists at the University of Athens say they have evidence that the ... research that could lead to one good one. Surviving Mesothelioma has just posted an ... The team evaluated 98 mesothelioma patients who got a second kind of ...
(Date:5/25/2016)... ... May 25, 2016 , ... Biohaven Pharmaceutical Holding Company Ltd. ... the company’s orphan drug designation request covering BHV-4157 for the treatment of Spinocerebellar ... FDA. , Spinocerebellar ataxia is a rare, debilitating neurodegenerative disorder that is ...
(Date:5/24/2016)... (PRWEB) , ... May 24, 2016 , ... Last week, ... for corporate executives and entrepreneurs, held The Future of San Diego Life Science event ... San Diego life science community attended the event with speakers Dr. Rich Heyman, former ...
Breaking Biology Technology:
(Date:3/18/2016)... , March 18, 2016 ... Suppliers of Biometrics, ICT, Manned & Unmanned Vehicles, Physical infrastructure ... & security companies in the border security market and the ... and Europe has led visiongain ... companies improved success. --> defence & security ...
(Date:3/15/2016)... ALBANY, New York , March 15, 2016 ... a new market report published by Transparency Market Research "Digital ... Growth, Trends and Forecast 2015 - 2023," the global digital ... at US$ 731.9 Mn in 2014 and is forecast to ... to 2023. Growth of micro, small and medium enterprises (MSMEs) ...
(Date:3/14/2016)... MELBOURNE, Florida , March 14, 2016 ... on the growing mobile commerce market, announces the airing of ... York channels starting the week of March 21 st . ... and CNBC, including its popular Squawk on the Street show. ... company focused on the growing mobile commerce market, announces the ...
Breaking Biology News(10 mins):