Navigation Links
Analysis of Phenolic Estrogen Mimics in River Water by Negative Ion Electrospray LC/MS/MS

Z. Yang, Varian, Inc.

Introduction

Bisphenol A (BPA) is a primary raw material used for the production of polyacrylates, epoxy resin, phenolic resin, and polycarbonate plastic. 4-Nonylphenol (4-NP) is a degradation product of alkylphenol ethoxylate detergents used in household and industrial cleaning products. Alkylphenol ethoxylate is also widely used as an additive in plastics that are used for food packaging and used as carriers in the application of pesticides to increase the absorption of these substances in the target organism.

BPA and 4-NP can leach from food containers and plastic tableware and can be ingested by humans through chronic exposure during eating and drinking. The chemicals have been found in drinking water supplies and wastewater.

Both BPA and 4-NP mimic the effect of natural estrogen hormones. This phenomenon raises the level of concern about the adverse health effects of these compounds on both humans and wildlife.

Instrumentation

Varian ProStar 430 AutoSampler

Varian ProStar 210 Isocratic Solvent Delivery Modules

Varian ProStar 510 Column Oven

Varian 1200L LC/MS equipped with ESI source

Materials and Reagents

Bisphenol A (Part No. 442840) and 4-Nonylphenol (Part No. 442873) supplied by Sigma-Aldrich Corp. (St. Louis, Missouri, U.S.A.).

All other chemicals are reagent grade or HPLC grade.

Sample Preparation

Samples were provided by a water company in Japan. The concentration of 4-NP and BPA were approximately 10 to 100 ng/mL and 1 to 10 ng/mL, respectively.

The samples were extracted with dichloromethane. The extracts were dried by a N2 stream and restored in the same volume of methanol/water (1:1, v/v) prior to analyses.

Results and Discussion

The LC method used a 12 minute cycle time. The data was acquired in SRM mode of two time segments (Figure 1).

Each standard calibration curve was generated using 6 points corresponding to a concentration range of 0.5 pg/μL* to 100 pg/μL (Figure 2). The linearity of the detector response was excellent with R2 0.999 for both BPA and 4-NP.

The method can quantitatively analyze both BPA and 4-NP at a level of 500 fg/μL in solution (Figure 1, bottom chromatogram). The limit of detection (LOD) is estimated to be about < 200 fg/μL in solution. The typical SRM chromatogram of water extract is given in Figure 1 (top chromatogram).

Conclusion

Estrogen mimics are included in a long list of chemicals targeted by various governmental and environmental groups. Complex matrices require instrumentation with good sensitivity and selectivity. In this evaluation, the use of a Varian 1200L LC/MS/MS system demonstrates how sensitive and selective tandem mass spectrometry is in the analyses of phenolic estrogen mimics. The 1200L can quantitatively analyze BPA and 4-NP at 500 fg/μL.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Epitope-Tagging Vectors for Functional Analysis in Yeast
3. Map and Link Human Genetic Disorders with SSLP Analysis
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
6. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
7. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
8. Increased Sensitivity in Microarray Analysis
9. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
(Date:7/7/2015)... ... 2015 , ... DNA Genotek, a leading provider of sample ... OSUR) today announced that its OMNIgene®•GUT microbiome collection kit has received CE-IVD marking ... Directive for In Vitro Diagnostic Medical Devices. , The CE-IVD marked OMNIgene•GUT ...
(Date:7/7/2015)... LANSING, Mich. , July 7, 2015  Neogen ... has signed an agreement to expand its relationship with ... sequencing and genomics. Under the agreement, Illumina will market ... on Illumina Infinium ® BeadArray technology, to the ... Illumina,s largest agrigenomic customers through its GeneSeek ® ...
(Date:7/7/2015)... ... July 07, 2015 , ... The global aptamers ... 2015, growing at a CAGR of 17.89% between 2015 and 2020. The aptamer ... aptamers. Based on applications, the report is segmented into diagnostics, therapeutics development, R&D, ...
(Date:7/7/2015)... (PRWEB) , ... July 07, 2015 , ... ... work together to lower barriers to innovation in life science R&D, has attracted ... the last quarter include another top ten pharmaceutical company joining as a Core ...
Breaking Biology Technology:DNA Genotek Inc. Receives CE-IVD Mark for its OMNIgene®•GUT Microbiome Collection Kit 2Neogen signs commercialization agreement with Illumina 2Aptamers Market Growing at 17.89% CAGR to 2020 – New Research Available at RnRMarketResearch.com 2Aptamers Market Growing at 17.89% CAGR to 2020 – New Research Available at RnRMarketResearch.com 3Aptamers Market Growing at 17.89% CAGR to 2020 – New Research Available at RnRMarketResearch.com 4Aptamers Market Growing at 17.89% CAGR to 2020 – New Research Available at RnRMarketResearch.com 5Pistoia Alliance Continues to Attract New Members 2
... SINGAPORE, April 21 /PRNewswire-Asia/ -- Moleac has been ... Frost & Sullivan. This award,recognizes companies that have ... and acknowledges Moleac,s outstanding performance in 2008.,Mr David ... & Sullivan Asia,Pacific Excellence in Healthcare Award for ...
... GenVault, a leading provider of room-temperature biosample transport, ... of GenConnect 3.2, the newest version of its ... the release include a strategic licensing model that ... informatics offerings, as well as a new customer ...
... ITMN ) announced today that it will release ... at 4:00 p.m. Eastern time. A live conference call ... Eastern time that same day. , , To ... conference ID# 95970767. To access the webcast, please log ...
Cached Biology Technology:Moleac Named Singapore Entrepreneurial Company of the Year at the Frost & Sullivan Excellence in Healthcare Awards 2Moleac Named Singapore Entrepreneurial Company of the Year at the Frost & Sullivan Excellence in Healthcare Awards 3GenVault Corporation Launches its Latest Centralized and Standardized Software Solution for Biobanking and Biological Sample Management, GenConnect 3.2 2GenVault Corporation Launches its Latest Centralized and Standardized Software Solution for Biobanking and Biological Sample Management, GenConnect 3.2 3InterMune to Release First Quarter Financial Results on April 30 2
(Date:6/24/2015)... 2015 This report provides market analysis of ... years. It contains an analysis of the drivers, challenges, ... impact from the short, medium, and long term perspective ... market, and technology trends that currently, prevail in the ... authorities to efficiently manage the increasing marine traffic and ...
(Date:6/23/2015)... 2015 Research and ... the "Body-Worn Temperature Sensors Market - Global ... 2014 - 2020" report to their offering. ... the global body-worn temperature sensors market. The global ... the basis of types, care setting, patient demographic, ...
(Date:6/17/2015)... HILDEN , Deutschland und GERMANTOWN, Maryland ... Investigator ®   Produktlinie mit ... QIAGEN N.V. (NASDAQ: QGEN ; Frankfurt Prime ... neue STR-Testkits der Produktlinie Investigator ... DNA-Spuren in den Markt eingeführt. Die neuen Kits zur ...
Breaking Biology News(10 mins):Automatic Identification System Market by Class, by Platform, by Application and by Geography - Global Forecasts & Analysis to 2014 - 2020 2Body-Worn Temperature Sensors Market - Global Industry Analysis 2QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 2QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 3QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 4QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 5QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 6
... biological clocks adjust to having less sunlight in the winter could ... work. Scientists studying the daily activity cycle in plants ... that enables the plant,s genes to respond to the times of ... daylight in between. This system helps the plant to reset ...
... Sivasankar was looking for a better tool to study how ... called cadherins, that help them stick together. Different kinds of ... observing and measuring those proteins focus on tens of thousands ... average molecule in a sample, but not on a single ...
... plants and pollinating insects hasten evolutionary changes and promote ... between plants and pollinators are less likely to increase ... it. Findings, published in the Journal of ... of the most distinctive plants in the Mojave Desert, ...
Cached Biology News:Iowa State, Ames Lab researcher develops new way to study single biological molecules 2Iowa State, Ames Lab researcher develops new way to study single biological molecules 3The puzzle of biological diversity 2
b6-2A12...
Request Info...
... polypeptide hormones which belong to the ... TGF- superfamily is a large group ... many aspects of development, reproductive function ... incude TGF-s, bone morphogenetic proteins, growth ...
... be able to handle larger sample volumes, automate ... as interface with telemetry systems. The AccuSampler ... dogs, pigs, and primates. The system may ... the computer can be located outside the animal ...
Biology Products: