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Analysis of Nitrofurans Metabolites by Positive Ion Electrospray LC/MS/MS

data produced that the 1200L LC/MS system can qualitatively and quantitatively detect nitrofurans to the ultra trace levels required. 10 mM ammonium acetate was used in the sample solution to reduce formation of sodium adducts and to enhance the detection limit for the product ions.

The Limit of Detection (LOD) for SC, AOZ, AH, and AMOZ is about 20 fg/μL*, 10 fg/μL, 60 fg/μL, and 10 fg/μL in solution, respectively. The linearity of the detector response was excellent (Figure 1). The R2 for 209>166, 236>134, 249>134 and 335>291 are 0.999, 0.998, 0.999 and 0.999, respectively. Typical chromatograms obtained are given in Figure 2.

Conclusion

The Varian 1200L LC/MS/MS system demonstrates excellent performance for the analyses of nitrofurans metabolites and is able to assist in the detection of potentially harmful substances in food imports/exports to improve food safety throughout the world.


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